In Vitro Evaluation of Enterococcus faecalis Adhesion on Various Endodontic Medicaments

Abstract

E. faecalis in endodontic infection represents a biofilm type of disease, which explains the bacteria’s resistance to various antimicrobial compounds and the subsequent failure after endodontic treatment. The purpose of this study was to compare antimicrobial activities and bacteria kinetic adhesion in vitro for three endodontic medicaments with a clinical isolate of E. faecalis. We devised a shake culture which contained the following intracanalar preparations: CPD, Endoidrox (EIX), PulpCanalSealer (PCS); these were immersed in a liquid culture medium inoculated with the microorganism. The shake system velocity was able to prevent non-specific bacteria adhesion and simulated the salivary flow. Specimens were collected daily (from both the medium and medicaments) for 10 days; the viable cells were counted by plate count, while the adhesion index AI° [E. faecalis fg DNA] /mm2 was evaluated in the pastes after DNA extraction, by quantitative real time PCR for the 16S rRNA gene. A partial growth inhibition, during the first 24 hours, was observed in the liquid medium and on the medicaments for EIX and subsequently for CPD (six logs). EIX showed the lowest adhesion coefficient (5*102 [fg DNA]/mm2) for nine days and was similar to the control. PCS showed no antimicrobial/antibiofilm properties. This showed that “calcium oxide” base compounds could be active against biofilm progression and at least in the short term (2-4 days) on E. faecalis cells growing in planktonic cultures.

Periodontal health in teenagers treated with removable aligners and fixed orthodontic appliances

“…1). The plaque and bleeding indices recorded on the incisor and the laren Protokolle wurden bereits in früheren Publikationen beschrieben [6,17] Bei der initialen Optimierung der Echtzeitreaktion wurden PCR-Produkte per Agarosegel und über Schmelzkurven analysiert, um eine korrekte Produktgröße der Proben zu gewährleisten. Die positiven Reaktionen zeigten TmSpitzen von 7-90°C.…”

“…The molecular protocols used in this study have been previously described [6,17]. Each paper point was suspended in 50 μl of pure dimethyl sulfoxide (DMSO) and centrifuged for 30 s; 2 µl of this was used as DNA suspension for realtime PCR reactions.…”

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“…After the incubation, the Aa was suspended in vials containing Schaedler broth to obtain a concentration with a turbidity equivalent to the No. 3 McFarland standard (about 10 8  CFU/mL) then diluted to 1/100 (obtaining a 10 6  CFU/mL) using a spectrophotometer at 620 nm (DMS100s, Varian, NH, USA) [19]. …”