In Vitro Evaluation of Enrofloxacin-Loaded MLV Liposomes

Sezer, Ali Demir; Akbuğa, Jülide; Baş, Ahmet Levent

doi:10.1080/10717540600640146

Cited by 21 publications

(13 citation statements)

References 20 publications

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“…We suggest that increasing the drug encapsulation capacity produced the slow release of ENR. This result was similar to the results found in the literature 3,13,14 . The optimized LUVs with the appropriate particle size and distribution, high surface charge and drug release properties used in the in vitro studies were chosen for use in the in vivo experiments, and the treatment efficacy on rats with experimental pneumonia was partially investigated.…”

Section: Discussionsupporting

confidence: 83%

“…In this study, ENR-loaded MLVs and LUVs, which were prepared using the optimum formulations of our previous studies 13,14 , were given to rats with experimental pneumonia, and we evaluated the efficiency of the liposomal treatment. The characterization of the liposomes' shape and surface was evaluated using a transmission electron microscope (TEM).…”

Section: Discussionmentioning

confidence: 99%

“…Preparation and Purification of Liposomes: ENR loaded multilamellar vesicles (MLVs) and large unilamellar vesicles (LUVs) were prepared using a modified dry film method 13,14 . The F-and L-ENR were separated by gel filtration on a Sephadex G-50 column at 25°C (1.0 cm in diameter, a gel bed height of 20 cm, Sigma Chemical Co., USA), and PBS (pH 7.4) was used as the medium 15 .…”

Section: Methodsmentioning

confidence: 99%

“…Size and zeta potential measurements were performed at 25°C using a Malvern Zetasizer (Nano ZS, Malvern Instruments, Worcestershire, UK) and the results are expressed as the mean of three measurements 14,16 .…”

Section: Determination Of Liposome Size and Zeta Potentialmentioning

confidence: 99%

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Cytologic-Enzymologic Diagnosis of Experimental Pneumonia Induced by Klebsiella pneumoniae Serotype II in Rats and Its Treatment with Free and Liposomal Enrofloxacin

Baş¹,

Üney²,

Hadimli³

et al. 2013

Kafkas Univ Vet Fak Derg

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SummaryEnrofloxacin (ENR) rapidly localizes in eukaryotic cells in vitro but does not remain for prolonged periods, thereby reducing the ENR efficacy of defense against intracellular pathogens. Delivery of ENR in a liposome-encapsulated form may enhance its intracellular residence time. In this study, experimental pneumonia was induced in healthy and dexamethasone-treated rats using Klebsiella pneumoniae serotype II. Free and liposome-encapsulated ENR were injected intravenously into the infected animals at a dose of 7.5 mg/kg/day for 5 days. Samples of tissue, plasma and bronchoalveolar lavage (BAL) fluid were obtained at 1, 2, 3 and 4 days and 1, 2, 3 and 4 weeks after the first antibiotic treatment. All of the samples were evaluated cytologically, enzymologically, microbiologically and pathologically. It was determined that cytologic and enzymologic diagnoses of BAL fluid are not meaningful for evaluating the treatment of the experimental pneumonia in rats. However, it was established that the use of ENR in liposomal form at a dose of 7.5 mg/kg for 5 days is more effective than the free form both in the treatment of K. pneumoniae infections and in the prevention of recurrent infections. Liposome-encapsulated antimicrobial agents should provide another choice for antimicrobial therapy in the future, but further investigation must be completed before clinical use. Keywords: Enrofloxacin, Experimental pneumonia, Klebsiella pneumoniae, Liposome, Rat, Treatment Ratlarda Klebsiella pneumoniae Serotip II İle Oluşturulan Deneysel Pnömonilerin Sitolojik-Enzimolojik Teşhisi ve Serbest ve Lipozomlanmış Enrofloksasin İle TedavisiÖzet Enrofloksasin (ENR) in vitro ortamlarda ökaryotik hücrelerde hızla lokalize olur, ancak uzun süre kalamadığı için hücre içi patojenlere karşı onun etkinliği azalır. Lipozomla kapsüle edilmiş formda ENR uygulanması hücre içi ortamlarda kalış zamanını artırabilir. Bu çalışmada, sağlıklı ve deksametazon uygulanmış ratlarda Klebsiella pneumoniae serotip II kullanılarak deneysel pnömoni oluşturuldu. Serbest ve lipozomla kapsüle edilmiş ENR enfekte hayvanlara intravenöz olarak beş gün boyunca 7.5 mg/kg/gün dozda enjekte edildi. İlk antibiyotik uygulamasından sonraki 1, 2, 3, 4. gün ve 1, 2, 3, 4. haftalarda plazma, doku ve bronko-alveolar lavaj (BAL) sıvısı örnekleri alındı. Tüm örnekler sitolojik, enzimolojik, mikrobiyolojik ve patolojik olarak değerlendirildi. Ratlarda deneysel pnömoninin tedavisinin değerlendirilmesinde BAL sıvısının sitolojik ve enzimolojik teşhisinin anlamsız olduğu belirlendi. Ancak, enrofloksasinin beş gün boyunca 7.5 mg/kg dozda lipozomal formda kullanımının hem K. pneumoniae enfeksiyonlarının tedavisinde hem de tekrarlayan enfeksiyonlarının önlenmesinde serbest formdan daha etkili olduğu tespit edildi. Lipozomla kapsüle edilmiş antimikrobiyal ajanlar gelecekte antimikrobiyal tedavide diğer bir seçenek sağlayacaklardır, ancak klinik kullanımdan önce çok sayıda araştırma yapılmalıdır.

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Section: Discussionsupporting

confidence: 83%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Determination Of Liposome Size and Zeta Potentialmentioning

confidence: 99%

See 2 more Smart Citations

Cytologic-Enzymologic Diagnosis of Experimental Pneumonia Induced by Klebsiella pneumoniae Serotype II in Rats and Its Treatment with Free and Liposomal Enrofloxacin

Baş¹,

Üney²,

Hadimli³

et al. 2013

Kafkas Univ Vet Fak Derg

Self Cite

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“…It was investigated that 85.7% of MTO was released from the MTO-TMLGNs after 72 hours, while almost 100% was released from MTO-Sol after 4 hours. It was possible that ionic interaction and complexation between the MTO and carrageenan was so strong Numerous methods for release determination are reported in the literature including dialysis-based methods, [35][36][37] ultracentrifugation, 38,39 centrifugal ultrafiltration, 40 and pressure ultrafiltration. 41,42 However, to our knowledge, no method is perfect for the release study to date.…”

Section: Release Characteristics Of Tmlgnsmentioning

confidence: 99%

Synergistic and complete reversal of the multidrug resistance of mitoxantrone hydrochloride by three-in-one multifunctional lipid-sodium glycocholate nanocarriers based on simultaneous BCRP and Bcl-2 inhibition

Ling¹,

Zhang²,

Zhang³

et al. 2016

IJN

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Multidrug resistance (MDR) is a severe obstacle to successful chemotherapy due to its complicated nature that involves multiple mechanisms, such as drug efflux by transporters (P-glycoprotein and breast cancer resistance protein, BCRP) and anti-apoptotic defense (B-cell lymphoma, Bcl-2). To synergistically and completely reverse MDR by simultaneous inhibition of pump and non-pump cellular resistance, three-in-one multifunctional lipid-sodium glycocholate (GcNa) nanocarriers (TMLGNs) have been designed for controlled co-delivery of water-soluble cationic mitoxantrone hydrochloride (MTO), cyclosporine A (CsA – BCRP inhibitor), and GcNa (Bcl-2 inhibitor). GcNa and dextran sulfate were incorporated as anionic compounds to enhance the encapsulation efficiency of MTO (up to 97.8%±1.9%) and sustain the release of cationic MTO by electrostatic interaction. The results of a series of in vitro and in vivo investigations indicated that the TMLGNs were taken up by the resistant cancer cells by an endocytosis pathway that escaped the efflux induced by BCRP, and the simultaneous release of CsA with MTO further efficiently inhibited the efflux of the released MTO by BCRP; meanwhile GcNa induced the apoptosis process, and an associated synergistic antitumor activity and reversion of MDR were achieved because the reversal index was almost 1.0.

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Bisdemethoxycurcumin-conjugated vitamin E TPGS liposomes ameliorate poor bioavailability of free form and evaluation of its analgesic and hypouricemic activity in oxonate-treated rats

et al. 2021

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In Vitro Evaluation of Enrofloxacin-Loaded MLV Liposomes

Cited by 21 publications

References 20 publications

Cytologic-Enzymologic Diagnosis of Experimental Pneumonia Induced by Klebsiella pneumoniae Serotype II in Rats and Its Treatment with Free and Liposomal Enrofloxacin

Cytologic-Enzymologic Diagnosis of Experimental Pneumonia Induced by Klebsiella pneumoniae Serotype II in Rats and Its Treatment with Free and Liposomal Enrofloxacin

Synergistic and complete reversal of the multidrug resistance of mitoxantrone hydrochloride by three-in-one multifunctional lipid-sodium glycocholate nanocarriers based on simultaneous BCRP and Bcl-2 inhibition

Bisdemethoxycurcumin-conjugated vitamin E TPGS liposomes ameliorate poor bioavailability of free form and evaluation of its analgesic and hypouricemic activity in oxonate-treated rats

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