In vitro evaluation of electrospun PCL/nanoclay composite scaffold for bone tissue engineering

Ganesh, Nitya; Nair, Greeshma T.; Mony, Ullas; Chennazhi, K.P.; Nair, Shantikumar V.

doi:10.1007/s10856-012-4647-x

Cited by 100 publications

(69 citation statements)

References 40 publications

(43 reference statements)

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“…Therefore, the incorporation of HNT favors the protein adsorption, which could be related to the difference in surface composition of nanofibers due to the incorporation of HNTs [52]. In fact, rough surface of nanofiber with high HNT content may also make partial contribution to the higher protein absorption [53].…”

Section: Mechanical Propertiesmentioning

confidence: 99%

Toughening of electrospun poly(l-lactic acid) nanofiber scaffolds with unidirectionally aligned halloysite nanotubes

et al. 2014

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The mechanical properties of the tissue engineering scaffold are important as they are tightly related the regeneration of structural tissue. The application of poly(L-lactic acid) (PLLA) nanofiber scaffolds in tissue engineering has been hindered by their insufficient mechanical properties. In the study, halloysite nanotubes (HNTs) were used to reinforce the mechanical properties of PLLA-based nanofibers. 4 wt% HNT/PLLA nanofiber membranes possess the best mechanical performance, which represents 61 % increase in tensile strength, 100 % improvement of Young's modulus, 49 % augment of elongation to break, as well as 181 % elevation in energy to break compared with neat PLLA samples. The satisfactory enhancement effect of HNTs can be attributed to the effective dispersion and incorporation of HNTs in PLLA matrix, which have been confirmed by the analysis of SEM, TEM, and FTIR. The addition of HNTs also improves the degree of crystallization and thermal stability of PLLA-based nanofibers. HNT-incorporated PLLA nanofiber membranes possess higher protein adsorption from fetal bovine serum than the neat PLLA specimen. Therefore, the introduction of HNTs can effectively enhance the mechanical properties of PLLA nanofiber scaffolds. HNT/PLLA nanofiber scaffolds possess potential application in skin tissue engineering.

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Section: Mechanical Propertiesmentioning

confidence: 99%

Toughening of electrospun poly(l-lactic acid) nanofiber scaffolds with unidirectionally aligned halloysite nanotubes

et al. 2014

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“…36 In a similar approach, the incorporation of halloysite nanoclay within electrospun PCL scaffolds increased the mechanical strength, protein adsorption, and cell adhesion of the resulting hybrid materials. 37 Despite interesting physical and chemical properties of clay-enriched nanocomposites, only a few studies have focused on using clay-based scaffolds for biomedical applications. 34,38,39 The addition of silicate nanoclay can be used to tune adhesion and spreading of fibroblast cells, preosteoblast cells, and hMSCs.…”

mentioning

confidence: 99%

Nanoclay-Enriched Poly(ɛ-caprolactone) Electrospun Scaffolds for Osteogenic Differentiation of Human Mesenchymal Stem Cells

Gaharwar

Mukundan²,

Karaca³

et al. 2014

Tissue Engineering Part A

133

122

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Musculoskeletal tissue engineering aims at repairing and regenerating damaged tissues using biological tissue substitutes. One approach to achieve this aim is to develop osteoconductive scaffolds that facilitate the formation of functional bone tissue. We have fabricated nanoclay-enriched electrospun poly(e-caprolactone) (PCL) scaffolds for osteogenic differentiation of human mesenchymal stem cells (hMSCs). A range of electrospun scaffolds is fabricated by varying the nanoclay concentrations within the PCL scaffolds. The addition of nanoclay decreases fiber diameter and increases surface roughness of electrospun fibers. The enrichment of PCL scaffold with nanoclay promotes in vitro biomineralization when subjected to simulated body fluid (SBF), indicating bioactive characteristics of the hybrid scaffolds. The degradation rate of PCL increases due to the addition of nanoclay. In addition, a significant increase in crystallization temperature of PCL is also observed due to enhanced surface interactions between PCL and nanoclay. The effect of nanoclay on the mechanical properties of electrospun fibers is also evaluated. The feasibility of using nanoclay-enriched PCL scaffolds for tissue engineering applications is investigated in vitro using hMSCs. The nanoclay-enriched electrospun PCL scaffolds support hMSCs adhesion and proliferation. The addition of nanoclay significantly enhances osteogenic differentiation of hMSCs on the electrospun scaffolds as evident by an increase in alkaline phosphates activity of hMSCs and higher deposition of mineralized extracellular matrix compared to PCL scaffolds. Given its unique bioactive characteristics, nanoclayenriched PCL fibrous scaffold may be used for musculoskeletal tissue engineering.

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“…Bonding defects between the alumina and silica lead to net negative and positive charges on the exterior and interior lumen, respectively [5,6]. HNTs are capable of enhancing the material properties of many polymer matrices [7][8][9][10].…”

Section: Introductionmentioning

confidence: 99%

Dry Sintered Metal Coating of Halloysite Nanotubes

et al. 2016

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Abstract:Halloysite nanotubes (HNTs) are a naturally-occurring aluminosilicate whose dimensions measure microns in length and tens of nanometers in diameter. Bonding defects between the alumina and silica lead to net negative and positive charges on the exterior and interior lumen, respectively. HNTs have been shown to enhance the material properties of polymer matrices and enable the sustained release of loaded chemicals, drugs, and growth factors. Due to the net charges, these nanotubes can also be readily coated in layered-depositions using the HNT exterior lumen's net negative charge as the basis for assembly. These coatings are primarily done through wet chemical processes, the majority of which are limited in their use of desired chemicals, due to the polarity of the halloysite. Furthermore, this restriction in the type of chemicals used often requires the use of more toxic chemicals in place of greener options, and typically necessitates the use of a significantly longer chemical process to achieve the desired coating. In this study, we show that HNTs can be coated with metal acetylacetonates-compounds primarily employed in the synthesis of nanoparticles, as metal catalysts, and as NMR shift reagents-through a dry sintering process. This method was capable of thermally decaying the metal acetylacetonate, resulting in a free positively-charged metal ion that readily bonded to the negatively-charged HNT exterior, resulting in metallic coatings forming on the HNT surface. Our coating method may enable greater deposition of coated material onto these nanotubes as required for a desired application. Furthermore, the use of chemical processes using toxic chemicals is not required, thus eliminating exposure to toxic chemicals and costs associated with the disposal of the resultant chemical waste.

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In vitro evaluation of electrospun PCL/nanoclay composite scaffold for bone tissue engineering

Cited by 100 publications

References 40 publications

Toughening of electrospun poly(l-lactic acid) nanofiber scaffolds with unidirectionally aligned halloysite nanotubes

Toughening of electrospun poly(l-lactic acid) nanofiber scaffolds with unidirectionally aligned halloysite nanotubes

Nanoclay-Enriched Poly(ɛ-caprolactone) Electrospun Scaffolds for Osteogenic Differentiation of Human Mesenchymal Stem Cells

Dry Sintered Metal Coating of Halloysite Nanotubes

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