In vitro evaluation of crosslinked electrospun fish gelatin scaffolds

Gomes, Susana R.; Rodrigues, Gabriela; Martins, Gabriel G.; Henriques, Célia; Silva, Jorge

doi:10.1016/j.msec.2012.12.014

Cited by 85 publications

(56 citation statements)

References 27 publications

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“…As is the case for EDC, GA participates in the construction of bioprosthetic heart valves, 26,43 inhibits proteases, 31 and modifies gelatins and other materials and tissues. 26,44,45 In dental treatments, GA has been used as a desensitizer that reacts with plasma proteins in dentin fluid to precipitate them, blocking dentin tubules. [46][47][48] Wiegand and others 49 showed that a GA-containing dentin desensitizer applied on 1.0-mm-thick dentin specimens under simulated pulpal flow conditions did not present toxic effects on L-929 fibroblasts.…”

Section: Discussionmentioning

confidence: 99%

Transdentinal Cytotoxicity of Carbodiimide (EDC) and Glutaraldehyde on Odontoblast-like Cells

Scheffel

Bianchi

Soares

et al. 2015

Operative Dentistry

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Carbodiimide (EDC) and glutaraldehyde are cross-linking agents capable of increasing resin-dentin bond durability. This study showed that these substances might be safely applied on acid-etched dentin since they have no transdentinal cytotoxic effects on cultured odontoblast-like cells. SUMMARYObjective: To evaluate the transdentinal cytotoxicity of three different concentrations of carbodiimide (EDC) or 5% glutaraldehyde (GA) on MDPC-23 cells.Methods: Seventy 0.4-mm-thick dentin disks obtained from human molars were adapted to artificial pulp chambers. MDPC-23 cells were seeded on the pulpal surface of the disks. After 48 hours, the occlusal dentin was acid-etched and treated for 60 seconds with one of the Results: EDC at all test concentrations did not reduce cell viability, while 5% GA did increase cell metabolism. Cell death by necrosis was not elicited by EDC or 5% GA. At the 24-hour period, 0.3 M and 0.5 M EDC reduced TP production by 18% and 36.8%, respectively. At seven days, increased TP production was observed in all groups. Collagen production at the 24-hour period was reduced when 0.5 M EDC was used. After seven days, no difference was observed among the groups. SEM showed no alteration in cell morphology or number, except in the hydrogen peroxide group.Conclusions: Treatment of acid-etched dentin with EDC or GA did not cause transdentinal cytotoxic effects on odontoblast-like cells.

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Section: Discussionmentioning

confidence: 99%

Transdentinal Cytotoxicity of Carbodiimide (EDC) and Glutaraldehyde on Odontoblast-like Cells

Scheffel

Bianchi

Soares

et al. 2015

Operative Dentistry

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“…6,7 In medical research GA is used in many different ways such as the construction of bioprosthetic heart valves, 8,9 modification of gelatins and other materials and tissues. 8,10,11 In dental field, it has been used to desensitize sensitive exposed dentin, 12 to inhibit MMPs 13 and to increase the mechanical properties of demineralized collagen prior to bonding procedures. 14 …”

Section: Introductionmentioning

confidence: 99%

Transdentinal cytotoxicity of glutaraldehyde on odontoblast-like cells

Scheffel

Soares

Basso

et al. 2015

Journal of Dentistry

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Objectives This study investigated the transdentinal cytotoxicity of glutahaldehyde-containing solutions/materials on odontoblast-like cells. Methods Dentin discs were adapted to artificial pulp chambers. MDPC-23 cells were seeded on the pulpal side of the discs and the occlusal surface was treated with the following solutions: water, 2%glutaraldehyde (GA), 5%GA, 10%GA, Gluma Comfort Bond+Desensitizer (GCB+De) or Gluma Desensitizer (GDe). Cell viability and morphology were assessed by the Alamar Blue assay and SEM. The eluates were collected and applied on cells seeded in 24-well plates. After 7 or 14 days the total protein (TP) production, alkaline phosphatase activity (ALP) and deposition of mineralized nodules (MN) were evaluated. Results Data were analyzed by Kruskal-Wallis and Mann-Whitney tests (p<0.05). GA solutions were not cytotoxic against MDPC-23. GCB+De (85.1%) and GDe (77.2%) reduced cell viability as well as TP production and ALP activity at both periods. After 14 days, GCB+De and GDe groups produced less MN. Affected MDPC-23 presented deformation of the cytoskeleton and reduction of cellular projections. Conclusions The treatment with 2.5%, 5% and 10%GA was not harmful to odontoblast-like cells. Conversely, when GA was combined with other components like HEMA, the final material became cytotoxic. Clinical significance Glutaraldehyde has been used to decrease dentin hypersensitivity. This substance is also capable of preventing resin-dentin bond degradation by cross-linking collagen and MMPs. This study showed that GA might be safe when applied on acid etched dentin. However, when combined with HEMA the product becomes cytotoxic.

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“…Preparation of solutions blending both types of polymer, which is the case of poly(ε‐caprolactone)/chitosan (PCL/CS) blends, is usually achieved through the use of aggressive and toxic solvents, like hexafluoro‐2‐propanol, trifluoroacetic acid, and trifluoroethanol . An alternative and less toxic solvent, acetic acid (AA), has also been used to prepare a few blends, like CS with collagen and poly(ethylene oxide) (PEO), CS with PCL and PEO and CS with PCL …”

Section: Introductionmentioning

confidence: 99%

“…There are reports in the literature of the production and evaluation of PCL/CS blends using AA as well as a few other solvents . Scaffolds have also been produced through the layer by layer deposition of PCL and CS fibers and through the simultaneous deposition of PCL and CS onto the same rotating collector .…”

Section: Introductionmentioning

confidence: 99%

Polymer blending or fiber blending: A comparative study using chitosan and poly(ε‐caprolactone) electrospun fibers

Valente

Ferreira

Henriques

et al. 2018

J of Applied Polymer Sci

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Nonwoven membranes of poly(ε‐caprolactone) (PCL) and chitosan (CS) were produced according to the two methods: by blending the polymers in solution followed by electrospinning – polymer blending method – and by simultaneous deposition of fibers electrospun from separate solutions – fiber blending (FB) method. The two production methods were compared by assessing fiber morphology, mass loss, swelling degree, water contact angle, and mechanical properties of the resulting electrospun membranes. Furthermore, the adhesion, proliferation, and morphology of human dermal fibroblasts on the eight types of scaffold produced were evaluated to assess if the blending method used would influence cell–scaffold interaction. Cell adhesion to the different scaffolds lied in the interval 40–60%, with the CS scaffold presenting the lowest value. Interestingly, cell proliferation was the same when comparing polymer blending and FB scaffolds having 3:1 or 1:3 PCL/CS ratios but very different when the ratio was 1:1 – the FB scaffold sustained a proliferation rate double that of the polymer blending scaffold. This work shows that, when blending polymers to improve the properties of a scaffold for tissue engineering or 3D cell culture, their spatial distribution may considerably affect scaffold's properties and should be considered as another parameter requiring optimization. © 2018 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2019, 136, 47191.

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In vitro evaluation of crosslinked electrospun fish gelatin scaffolds

Cited by 85 publications

References 27 publications

Transdentinal Cytotoxicity of Carbodiimide (EDC) and Glutaraldehyde on Odontoblast-like Cells

Transdentinal Cytotoxicity of Carbodiimide (EDC) and Glutaraldehyde on Odontoblast-like Cells

Transdentinal cytotoxicity of glutaraldehyde on odontoblast-like cells

Polymer blending or fiber blending: A comparative study using chitosan and poly(ε‐caprolactone) electrospun fibers

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