In vitro evaluation of Biosilicate® dissolution on dentin surface: a SEM analysis

Abstract

INTRODUCTION: Biomaterials such as bioactive glasses and glass-ceramics have been proposed for the treatment of dentinal hypersensitivity. OBJECTIVE: to evaluate by scanning electron microscopy (SEM), the dissolution of a novel bioactive glass-ceramic (Biosilicate® 1-20 µm particles) on dentin surface samples, with different application methods and different dilution medium used for applying Biosilicate®. MATERIAL AND METHOD: 280 dentin samples were randomly divided into four groups: (1) Biosilicate® plus fluoride gel applied with Robinson brush; (2) Biosilicate® plus fluoride gel applied with microbrush; (3) Biosilicate® plus distilled water applied with Robinson brush; (4) Biosilicate® plus distilled water applied with microbrush. After treatment, the samples were immersed in saliva at different periods (0, 15 and 30 minutes, 1, 2, 12 and 24 hours). Two photomicrographs were obtained from each sample and were further analyzed by a blind calibrated examiner according to a "Particle Dissolution Index" created for this study. RESULT: The data were analyzed using the Mann-Whitney, Kruskal-Wallis and Dunn's tests. There was no statistical difference among the degrees of dissolution between the 4 groups in any period. CONCLUSION: Biosilicate® can be incorporated in both substances without differences in the degree of dissolution of the particles in any of the evaluated periods and the application of dentine can be performed with both methods evaluated.

Effect of bioactive glasses used as dentin desensitizers on the dentin-pulp complex in rats

“…The topical application of these substances on exposed dentinal tubules cannot generate unwanted and inadvertent pulpal inflammation. Several in vitro and clinical studies have shown the efficacy of Biosilicate ® parent glass in the occlusion of dentinal tubules 22,31,32) . An experimental bioglass containing strontium was used in the present study because in vitro studies using strontium salts, mainly strontium chloride or acetate in dentifrices, have demonstrated strontium ion affinity to dentin, with positive effects on the occlusion of dentinal tubules and dentin permeability.…”

Efficacy of a bioactive material and nanostructured desensitizing on dentin hypersensitivity treatment

“…Its chemical composition is P 2 O 5 -Na 2 O-CaO-SiO 2 and is biocompatible 20,21,23 . The bioactive glass-ceramic, in contact with fluids, starts the chemical reaction.…”

“…Thus, when this material was applied on the dentin surface, it showed deposition of hydroxycarbonate apatite, occluding the open dentinal tubules. It could be a promising desensitizing agent for the treatment of dentin hypersensitivity 23,24 . Similar to the results of the present investigation, a previous study also found greater absolute pain reduction after biosilicate application 22 .…”

“…Initially, the area to be treated was relatively isolated. A prior mechanical cleaning was performed in the sensitive area using a cotton swab soaked in distilled water 23 . Then, a stream of compressed air (~ 10 ºC, 40-65 psi) was blown for 2 s, at 1 cm, perpendicular to the tooth surface while adjacent teeth were protected by the operator's gloved fingers and cotton rolls.…”

“…This change results in particles with lower cutting potential and proven biological properties 20,21 , reducing dentin hypersensitivity 22 . Previous studies have shown that, after biosilicate application on the dentin, deposition of hydroxycarbonate apatite in open dentinal tubules was observed, forming a uniform layer over this surface 23,24 .…”