2018
DOI: 10.31024/ajpp.2018.4.6.25
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In vitro evaluation of antioxidant activity of D-Limonene

Abstract: Objective: Reactive oxygen species (ROS) and free radicals are involved in pathogenesis of cancer. D-Limonene, a monoterpene present in citrus fruit has been reported for its potential anticancer activities. The aim of present study was to evaluate the antioxidant effect of D-Limonene. Test solutions with different in vitro Material and methods: concentration of D-Limonene and Trolox (standard) were prepared. Six different antioxidant assays such as In vitro DPPH, ABTS, FRAP, iron chelating, hydroxyl radical s… Show more

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Cited by 38 publications
(37 citation statements)
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References 21 publications
(29 reference statements)
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“…The in vitro antioxidant activity of d ‐limonene was evaluated by six different antioxidant assays, that is DPPH, ABTS, FRAP, iron chelating, hydroxyl and superoxide radical scavenging using Trolox as a positive control. d ‐limonene possessed significant antioxidant activity with IC 50 values for different assays were 384.73, 603.23, −589.85, −18475.5, 442.75 and 692.89 µmol/L, respectively, whereas for Trolox, the IC 50 values were found to be 153.30, 203.37, −171.73, 225.96, 146.37 and 105.25 µmol/L, respectively 89 …”
Section: Limonenementioning
confidence: 96%
“…The in vitro antioxidant activity of d ‐limonene was evaluated by six different antioxidant assays, that is DPPH, ABTS, FRAP, iron chelating, hydroxyl and superoxide radical scavenging using Trolox as a positive control. d ‐limonene possessed significant antioxidant activity with IC 50 values for different assays were 384.73, 603.23, −589.85, −18475.5, 442.75 and 692.89 µmol/L, respectively, whereas for Trolox, the IC 50 values were found to be 153.30, 203.37, −171.73, 225.96, 146.37 and 105.25 µmol/L, respectively 89 …”
Section: Limonenementioning
confidence: 96%
“…It is toxic for some herbivore species [ 43 ] and is an allelopathic agent in hot and dry climates [ 44 ]. Moreover, limonene has chemopreventive, antitumoral [ 45 ], hypoglycemic [ 46 ], antioxidant [ 47 , 48 ] anti-inflammatory [ 46 ], antibacterial [ 49 ], and antifungal [ 50 ] properties.…”
Section: Discussionmentioning
confidence: 99%
“…Among the identified volatiles, some of them were previously tested for their antioxidant properties. For example, limonene showed a concentration-dependent reduction in free radical formation through various in vitro assays [ 38 ]. Tested together with other terpenoids, it has been shown to be among the most potent scavengers of the free radical DPPH as well as having one of the highest reducing powers [ 39 ].…”
Section: Discussionmentioning
confidence: 99%