In Vitro Endothelialization of PTFE Vascular Grafts: A Comparison of Various Substrates, Cell Densities, and Incubation Times

Vinard, E.; Lesèche, Guy; Andréassian, B; Costagliola, Danièle

doi:10.1007/s100169900232

Cited by 22 publications

(15 citation statements)

References 22 publications

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“…These observations could explain the differences of results between these two supports, which have different physical and chemical properties. Fibronectin is a biological material, which presents some limits (as indicated in the introduction section) and risks, since extracted from blood, of the transmission of virus 51. The build‐up on the vascular graft luminal surface with such PMF has the main advantages to be easily realized (by simple alternated polyelectrolyte solution flowing) and finally induce only a minimal reduction of the cross‐section (film with few tens of nanometers thickness).…”

Section: Resultsmentioning

confidence: 99%

Polyelectrolyte multilayer film and human mesenchymal stem cells: An attractive alternative in vascular engineering applications

Moby

Labrude

Kadi

et al. 2010

J Biomedical Materials Res

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Mesenchymal stem cells (MSCs) have tremendous potential as a cell source for regenerative medicine due to their capacity for differentiation into endothelial-like cells when seeded on nonmodified cover glasses. This absence of removable surface, preventing recovery of cell sheet, constitutes a critical obstacle to predict an application in tissue engineering. It remains unknown whether MSCs differentiation could be realized when the cells are cultivated on a scaffold that could be used in vascular engineering. In this study, we propose to differentiate human MSCs into endothelial-like cells on surfaces coated with polyelectrolyte multilayer film (PMF) and fibronectin (control surfaces). We quantified Platelet Endothelial Cell Adhesion Molecule (PECAM) and von Willebrand Factor (vWF) expressions (endothelial cell specific markers) and nitric oxide (NO) production, which is representative of the cell functionality. After only two weeks of differentiation, we showed, on PMF, that MSCs expressed PECAM and vWF, exhibiting a differentiation into endothelial-like cells, which functionality was explored by a significant production of nitrites. These results highlight the importance of PMF to get human MSCs differentiation and suggest that this film of nanometer thickness opens a new route for vascular bioengineering by pre-seeding hMSCs directly into a vascular graft functionalized by a removable coating.

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Section: Resultsmentioning

confidence: 99%

Polyelectrolyte multilayer film and human mesenchymal stem cells: An attractive alternative in vascular engineering applications

Moby

Labrude

Kadi

et al. 2010

J Biomedical Materials Res

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show abstract

“…To improve their long-term biocompatibility and hemocompatibility, it is necessary to recreate an internal environment similar to that found in native vessels. [6][7][8][9] For such an objective, EC seeding and tissue engineering techniques have been previously developed. 10 However, EC seeding on ePTFE has limited success due to the existence of electrostatic repulsive interactions between the negatively charged ECs and the negatively charged graft material.…”

Section: Introductionmentioning

confidence: 99%

Poly(styrenesulfonate)/Poly(allylamine) Multilayers: A Route To Favor Endothelial Cell Growth on Expanded Poly(tetrafluoroethylene) Vascular Grafts

et al. 2007

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Small-diameter synthetic vascular grafts of expanded poly(tetrafluoroethylene) (ePTFE) polymer concern one of the most common alternatives for the replacement of diseased vessels. However, high failure rates arise especially due to the lack of endothelial cells (ECs). EC seeding was developed to build a monolayer on the luminal surface. Because ECs show little or no adhesion on synthetic prostheses, it is necessary to promote their retention. On ePTFE surfaces we successfully deposited polyelectrolyte multilayer films (PMFs) consisting of poly(ethylenimine) (PEI), poly(sodium 4-styrenesulfonate) (PSS), and poly(allylamine hydrochloride) (PAH) to obtain PEI-(PSS-PAH)3 films. EC adhesion and spreading on modified ePTFE were assessed by scanning electron and confocal microscopies. Cell viability was evaluated by Alamar Blue assay. After 7 days of culture, the ePTFE modified with PMF exhibited improvements of EC viability as compared to that of the controls (nonmodified ePTFE) or even ePTFE coated by a PAH monolayer (p < 0.05). Moreover, the spreading of ECs was largely enhanced compared to that of the same controls, resulting in a healthy confluent cell monolayer formation. Positive staining for the von Willebrand factor confirmed the EC phenotype. Promoting EC attachment and function on ePTFE modified with PMFs could become in the future a promising treatment for synthetic small-diameter vascular grafts.

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“…Unfortunately, synthetic small diameter vascular grafts have unacceptable patency rates primarily due to lumenal thrombus formation and intimal thickening [3]. Lumenal endothelialization represents a clear, immediate, and practical solution to poor graft patency [4]. However, the promise of this approach has not been realized because of numerous unresolved issues concerning seeding efficiency, cell retention under flow, and achieving a quiescent and anti-thrombotic endothelial cell (EC) phenotype that resists thrombosis, intimal hyperplasia, and leukocyte adhesion [5][6][7][8].…”

Section: Introductionmentioning

confidence: 99%

Mylar™ and Teflon-AF™ as cell culture substrates for studying endothelial cell adhesion

2005

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In Vitro Endothelialization of PTFE Vascular Grafts: A Comparison of Various Substrates, Cell Densities, and Incubation Times

Cited by 22 publications

References 22 publications

Polyelectrolyte multilayer film and human mesenchymal stem cells: An attractive alternative in vascular engineering applications

Polyelectrolyte multilayer film and human mesenchymal stem cells: An attractive alternative in vascular engineering applications

Poly(styrenesulfonate)/Poly(allylamine) Multilayers: A Route To Favor Endothelial Cell Growth on Expanded Poly(tetrafluoroethylene) Vascular Grafts

Mylar™ and Teflon-AF™ as cell culture substrates for studying endothelial cell adhesion

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