In vitro Endothelialization and Platelet Adhesion on Titaniferous Upgraded Polyether and Polycarbonate Polyurethanes

Abstract

Polycarbonateurethanes (PCU) and polyetherurethanes (PEU) are used for medical devices, however their bio- and haemocompatibility is limited. In this study, the effect of titaniferous upgrading of different polyurethanes on the bio- and haemocompatibility was investigated by endothelial cell (EC) adhesion/proliferation and platelet adhesion (scanning electron microscopy), respectively. There was no EC adhesion/proliferation and only minor platelet adhesion on upgraded and pure PCU (Desmopan). PEUs (Texin 985, Tecothane 1085, Elastollan 1180A) differed in their cyto- and haemocompatibility. While EC adhesion depended on the type of PEU, any proliferative activity was inhibited. Additional titaniferous upgrading of PEU induced EC proliferation and increased metabolic activity. However, adherent ECs were significantly activated. While Texin was highly thrombotic, only small amounts of platelets adhered onto Tecothane and Elastollan. Additional titaniferous upgrading reduced thrombogenicity of Texin, preserved haemocompatibility of Elastollan, and increased platelet activation/aggregation on Tecothane. In conclusion, none of the PUs was cytocompatible; only titaniferous upgrading allowed EC proliferation and metabolism on PEUs. Haemocompatibility depended on the type of PU.

Plasma functionalization of polycarbonaturethane to improve endothelialization—Effect of shear stress as a critical factor for biocompatibility control

“…Furthermore, a third part of the grafts was used for scanning electron microscopy (SEM) as described by Lehle et al. 38…”

“…Other sections of the grafts were stained with rhodamine-phalloidin (see above) and with fluorescein isothiocyanate (FITC)-conjugated monoclonal anti-human CD31 antibody/ Ancell, Bayport, MN). Furthermore, a third part of the grafts was used for scanning electron microscopy (SEM) as described by Lehle et al 38 …”

Ultrastructural Study of Platelet Behavior and Interrelationship in Sprouting and Intussusceptive Angiogenesis during Arterial Intimal Thickening Formation

“…Platelet participation in several pathophysiological mechanisms involved in atherosclerosis, bypass stenosis, and restenosis is an important subject of research in human and experimental pathology [ 1 , 2 , 3 , 4 , 5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 , 13 , 14 ]. Platelets act in angiogenesis [ 15 , 16 , 17 , 18 ], which is one of the main pathophysiological mechanisms of this pathology.…”

Friction Stir Processing of Stainless Steel for Ascertaining Its Superlative Performance in Bioimplant Applications

“…Scanning Electron Microscopy (SEM) Protocol. Platelet adhesion on the 1800C specimen was examined by a scanning electron microscope (SEM) as reported, 38 with a slight modification obtained from Fischer et al 39 Briefly, platelets isolated from human citrated blood were coincubated with the specimen for 1 h at 37 °C and 5% CO 2 . Platelets incubated on coverslips were used as control.…”