In vitro embryo production in wood bison (Bison bison athabascae) using in vivo matured cumulus-oocyte complexes

Cervantes, Miguel; Palomino, J. M.; Anzar, M.; Mapletoft, R.J.; Mastromonaco, Gabriela F.; Adams, Gregg P.

doi:10.1016/j.theriogenology.2016.10.017

Cited by 10 publications

(7 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Female wood bison ( n = 40) were assigned randomly to two groups in which a single dose of hCG (Cervantes et al, ) was given 48 or 72 hr after the last superstimulatory dose of FSH during both the anovulatory and the ovulatory seasons to induce in vivo maturation of COC (Figure ). During the anovulatory season, ovarian follicular development was synchronized among bison by follicular ablation (Day‐1) and ovarian superstimulation was induced by intramuscular administration of 200 mg FSH diluted in 0.5% of hyaluronan on Days 0 and 2.…”

Section: Methodsmentioning

confidence: 99%

“…Intramuscular administration of 2,500 IU hCG was given at either 48 or 72 hr after the last dose of FSH ( n = 10/group). COC were collected 34 hr after hCG treatment, as previously described (Cervantes et al, ; Palomino, McCorkell, Woodbury, Cervantes, & Adams, ; Palomino, McCorkell, Woodbury, Cervantes, et al, ). During the ovulatory season, treatments were done as described for the anovulatory season, except that bison were given a luteolytic dose of prostaglandin i.m.…”

Section: Methodsmentioning

confidence: 99%

“…In both seasons, bison were scanned as described in Experiment 1. Collected COC were evaluated, classified (expanded, compact and denuded) and maintained in holding medium for further processing (Cervantes et al, ).…”

Section: Methodsmentioning

confidence: 99%

“…Embryo production via techniques involving in vivo and in vitro fertilization is necessary to deploy a genome biobank and has been used to a limited extent in the preservation of some endangered wildlife species (Comizzoli, Mermillod, & Mauget, ; Loskutoff, Bartel, Meintjes, Godke, & Schiewe, ). Beginning with the bovine model (Adams & Singh, ), we have conducted a series of studies in an effort to develop these reproductive technologies for use in bison (Cervantes, Palomino, Anzar, Mapletoft, & Adams, ; Cervantes et al, , ; Palomino, Cervantes, & Adams, ; Palomino, McCorkell, Woodbury, & Adams, ; Palomino et al, , ; Toosi et al, ). An added complexity with North American bison is seasonality.…”

Section: Introductionmentioning

confidence: 99%

“…Early studies on in vitro embryo production involved the use of oocytes collected from slaughterhouse‐derived bison ovaries, with modest results, and suggest that oocytes collected during the anovulatory season have lower developmental competence than those collected during the ovulatory season (Barfield & Seidel, ; Thundathil et al, ). More recent studies using oocytes collected transvaginally from live superstimulated bison during both the ovulatory and anovulatory seasons have demonstrated an improvement in the production of embryos in vitro (Cervantes et al, ).…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Effect of season and superstimulatory treatment on in vivo and in vitro embryo production in wood bison (Bison bison athabascae)

Palomino

Mastromonaco

Cervantes

et al. 2019

Reprod Domestic Animals

Self Cite

View full text Add to dashboard Cite

Two experiments were done using a two-by-two design to determine the effects of season and superstimulatory protocol on embryo production in wood bison. In Experiment 1 (in vivo-derived embryos), ovarian superstimulation was induced in female bison during the ovulatory and anovulatory seasons with either two or three doses of FSH given every-other-day (FSH × 2 vs. FSH × 3, respectively). Bison were given hCG to induce ovulation, inseminated 12 and 24 hr after hCG, and embryos were collected 8 days after hCG (n = 10 bison/group). In Experiment 2 (in vitro embryo production), ovarian superstimulation was induced in female bison during the ovulatory and anovulatory seasons with two doses of FSH, and in vivo maturation of the cumulus-oocyte complexes (COC) was induced with hCG at either 48 or 72 hr after the last dose of FSH. COC were collected 34 hr after hCG, and expanded COC were used for in vitro fertilization and culture. In Experiment 1, the number of follicles ≥9 mm, the proportion of follicles that ovulated, the number of CL, and the total number of ova/embryos collected did not differ between seasons or treatment groups, but the number of transferable embryos was greater (p < .05) in the ovulatory season. In Experiment 2, no differences were detected between seasons or treatment groups for any end point. The number of transferable embryos produced per bison was greatest (p < .05) using in vitro fertilization and was unaffected by season (1.5 ± 0.2 and 1.1 ± 0.3 during anovulatory and ovulatory seasons, respectively), in contrast to in vivo embryo production which was affected by season (0.1 ± 0.01 and 0.7 ± 0.2 during anovulatory and ovulatory seasons, respectively). Results demonstrate that transferable embryos can be produced throughout the year in wood bison by both in vivo and in vitro techniques, but the efficiency of embryo production of in vivo-derived embryos is significantly lower during the anovulatory season. K E Y W O R D S FSH, in vitro embryo, in vivo embryo, season, wood bison | 55 PALOMINO et AL. How to cite this article: Palomino JM, Mastromonaco GF, Cervantes MP, Mapletoft RJ, Anzar M, Adams GP. Effect of season and superstimulatory treatment on in vivo and in vitro embryo production in wood bison (Bison bison athabascae).

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%