In Vitro Production of Bison Embryos

Barfield, Jennifer P.

doi:10.1007/978-1-4939-9566-0_12

Cited by 4 publications

(1 citation statement)

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“…In a recent study testing the effectiveness of an extended FSH treatment protocol, no differences were found between 2 versus 3 treatments of FSH given at 48‐hr intervals during the anovulatory season, but during the ovulatory season, embryo collection efficiency was greater in the extended treatment group resulting in a greater number of transferable embryos (Palomino, Cervantes, Mapletoft, Woodbury, & Adams, ). Early studies on in vitro embryo production involved the use of oocytes collected from slaughterhouse‐derived bison ovaries, with modest results, and suggest that oocytes collected during the anovulatory season have lower developmental competence than those collected during the ovulatory season (Barfield & Seidel, ; Thundathil et al, ). More recent studies using oocytes collected transvaginally from live superstimulated bison during both the ovulatory and anovulatory seasons have demonstrated an improvement in the production of embryos in vitro (Cervantes et al, ).…”

Section: Introductionmentioning

confidence: 99%

Effect of season and superstimulatory treatment on in vivo and in vitro embryo production in wood bison (Bison bison athabascae)

Palomino

Mastromonaco

Cervantes

et al. 2019

Reprod Domestic Animals

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Two experiments were done using a two-by-two design to determine the effects of season and superstimulatory protocol on embryo production in wood bison. In Experiment 1 (in vivo-derived embryos), ovarian superstimulation was induced in female bison during the ovulatory and anovulatory seasons with either two or three doses of FSH given every-other-day (FSH × 2 vs. FSH × 3, respectively). Bison were given hCG to induce ovulation, inseminated 12 and 24 hr after hCG, and embryos were collected 8 days after hCG (n = 10 bison/group). In Experiment 2 (in vitro embryo production), ovarian superstimulation was induced in female bison during the ovulatory and anovulatory seasons with two doses of FSH, and in vivo maturation of the cumulus-oocyte complexes (COC) was induced with hCG at either 48 or 72 hr after the last dose of FSH. COC were collected 34 hr after hCG, and expanded COC were used for in vitro fertilization and culture. In Experiment 1, the number of follicles ≥9 mm, the proportion of follicles that ovulated, the number of CL, and the total number of ova/embryos collected did not differ between seasons or treatment groups, but the number of transferable embryos was greater (p < .05) in the ovulatory season. In Experiment 2, no differences were detected between seasons or treatment groups for any end point. The number of transferable embryos produced per bison was greatest (p < .05) using in vitro fertilization and was unaffected by season (1.5 ± 0.2 and 1.1 ± 0.3 during anovulatory and ovulatory seasons, respectively), in contrast to in vivo embryo production which was affected by season (0.1 ± 0.01 and 0.7 ± 0.2 during anovulatory and ovulatory seasons, respectively). Results demonstrate that transferable embryos can be produced throughout the year in wood bison by both in vivo and in vitro techniques, but the efficiency of embryo production of in vivo-derived embryos is significantly lower during the anovulatory season. K E Y W O R D S FSH, in vitro embryo, in vivo embryo, season, wood bison | 55 PALOMINO et AL. How to cite this article: Palomino JM, Mastromonaco GF, Cervantes MP, Mapletoft RJ, Anzar M, Adams GP. Effect of season and superstimulatory treatment on in vivo and in vitro embryo production in wood bison (Bison bison athabascae).

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Section: Introductionmentioning

confidence: 99%