In vitro elimination of Black raspberry necrosis virus from black raspberry (Rubus occidentalis) - Short Communication

Cheong, Eun Ju; Jeon, Ae Rin; Kang, Jun Won; Mock, Ray; Kinard, Gary; Li, Ruhui

doi:10.17221/266/2013-hortsci

Cited by 5 publications

(5 citation statements)

References 23 publications

(25 reference statements)

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“…Working on combining thermotherapy with shoot tip culture for virus eradication from pear, Tan et al [ 63 ] found that survival levels, shoot length and proliferation efficiency of in vitro shoots decreased, while virus-free frequencies of ACLSV and ASPV increased, as thermotherapy durations increased from 10 to 50 days. Negative effects of prolonged thermotherapy durations on in vitro shoots and shoot tips excised from the treated shoots were frequently found in woody plants such as apricot [ 64 ], peach [ 64 ], cherry [ 64 ], pear [ 65 , 66 ], plum [ 62 ] and black raspberry [ 67 ], and herbaceous species such as horseradish [ 58 ], chrysanthemum [ 33 ], begonia [ 63 ] and potato [ 64 ]. Increased virus eradication frequencies by increasing thermotherapy durations were also frequently reported in various virus-host combinations.…”

Section: Key Factors Affecting Success Of Virus Eradicationmentioning

confidence: 99%

“…SA treatments decreased catalase activity and increased hydrogen peroxide (H 2 O 2 ) levels of the in vitro potato shoots, thus enhancing their tolerance to thermotherapy [ 65 , 66 ]. SA induced plant defense to virus infection and was beneficial for virus eradication [ 67 ]. Therefore, SA had double positive effects in thermotherapy for virus eradication: enhancing plant tolerance to thermotherapy and increasing virus eradication frequency.…”

Section: Key Factors Affecting Success Of Virus Eradicationmentioning

confidence: 99%

“…Therefore, SA had double positive effects in thermotherapy for virus eradication: enhancing plant tolerance to thermotherapy and increasing virus eradication frequency. Detailed information of SA-mediated biotic and abiotic stress signalling in plants can be found in a recent review [ 67 ].…”

Section: Key Factors Affecting Success Of Virus Eradicationmentioning

confidence: 99%

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In vitro thermotherapy-based methods for plant virus eradication

Wang

Cui

et al. 2018

Plant Methods

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Production of virus-free plants is necessary to control viral diseases, import novel cultivars from other countries, exchange breeding materials between countries or regions and preserve plant germplasm. In vitro techniques represent the most successful approaches for virus eradication. In vitro thermotherapy-based methods, including combining thermotherapy with shoot tip culture, chemotherapy, micrografting or shoot tip cryotherapy, have been successfully established for efficient eradication of various viruses from almost all of the most economically important crops. The present study reviewed recent advances in in vitro thermotherapy-based methods for virus eradication since the twenty-first century. Mechanisms as to why thermotherapy-based methods could efficiently eradicate viruses were discussed. Finally, future prospects were proposed to direct further studies.

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Section: Key Factors Affecting Success Of Virus Eradicationmentioning

confidence: 99%

Section: Key Factors Affecting Success Of Virus Eradicationmentioning

confidence: 99%

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In vitro thermotherapy-based methods for plant virus eradication

Wang

Cui

et al. 2018

Plant Methods

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“…Correlation coefficient among plantlet survival after heat treatment (%), meristems survival (%) in tissue culture at 30 days after excision, meri-clone regeneration after 45 days (%) and virus free meri-clones (%). meristem regeneration efficiencies implying differences in cultivars for meristem culture and meri-clone regeneration (Cheong et al, 2014;Danci et al, 2012;Biniam and Tadesse, 2008). Virus-free meri-clones were obtained in both heat treated and controls plantlets, however, the virus-free success rate was higher in the former than the latter.…”

Section: Discussionmentioning

confidence: 99%

Effect of thermotherapy duration, virus type and cultivar interactions on elimination of potato viruses X and S in infected seed stocks

Waswa¹,

Kakuhenzire²,

Ochwo-Ssemakula³

2017

Afr. J. Plant Sci.

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Virus infection in potato reduces yield through seed degeneration. This can be reduced by use of virusfree seed tubers. However, novel approaches are required to ensure availability of virus-free stocks, especially in developing countries where the seed potato system are evolving. Consequently, a laboratory experiment was conducted at Kachwekano ZARDI in Uganda to evaluate and determine appropriate procedures for cleaning valuable potato cultivars infected with the most prevalent potato viruses; potato virus X (PVX) and potato virus S (PVS) which are frequent in the potato farming system. Thus, 20 in-vitro potato plantlets from cultivars Victoria, Kinigi and Rwangume each batch infected with either PVX or PVS in three replicates were grown in a thermotherapy chamber for two, three and four weeks at 37 to 40°C in 16 h of light and 30 to 34°C for 8 h of darkness per day. An equal number plantlets and replicates of the same cultivars and virus infection combinations were grown in a standard tissue culture (TC) growth room at 16 to 18°C with 16 h of light without thermotherapy as controls. Results indicated that plantlet survival after in-vitro thermotherapy decreased with increasing duration of heat treatment. Virus elimination efficiency significantly (P ≤ 0.05) differed between heattreated plantlets and the controls but not between the duration of thermotherapy treatment. However, the highest proportion of virus-free plantlets was obtained after three weeks of thermotherapy. Significant (P ≤ 0.05) interactions were observed between thermotherapy duration and virus type on virus elimination efficiency where significantly (P ≤ 0.05) more PVS-free than PVX-free plantlets were obtained at the same thermotherapy duration. Three weeks of thermotherapy of virus-infected in-vitro plantlets, particularly for PVS, offered an equilibrium duration for adequate plantlet survival and maximum meri-clone regeneration to obtain the highest proportion of virus-free plantlets. The use of thermotherapy to obtain a high proportion of PVX-free plantlets from infected potato seed stock showed no clear trend and needs further investigation.

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“…The most commonly used procedures for viruses and viroids are meristem culture, somatic embryogenesis combined or not with thermotherapy, and chemotherapy (Panattoni et al, 2013;Parštein et al, 2013;Cheong et al, 2014). For instance, in grape, meristem culture has been used for the production of viroid-free grapevines (DuranVila et al, 1988) and also to clean plants infected with GFLV, GLRaV-1, and GLRaV-3 (Youssef et al, 2009;Shatnawi et al, 2011).…”

Section: Embryogenesis Protocol For Sanitation Of Grapementioning

confidence: 99%

Mature seeds for in vitro sanitation of the Grapevine leafroll associated virus (GLRaV-1 and GLRaV-3) from grape (Vitis vinifera L.)

Peiró

Gammoudi

Yuste

et al. 2015

Span. j. agric. res.

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<p>The conservation of old grapevine varieties is important since they are adapted to specific climate conditions and may carry genes interesting to breeders. As virus infection is common in grapevine varieties, the use of virus free materials is of great importance. In this work, we used somatic embryogenesis for the sanitation of GLRaV-1 and GLRaV-3 viruses that were found after analyzing the putative presence of the five most common, economically important grape viruses by real-time multiplex RT-PCR in the old cultivar “Grumet Negre”. Unopened and opened inflorescences, fecundated ovaries, and, also, mature seeds were used as starting explants. Explants were<em> </em>cultured on plates with two embryogenesis induction media (Nitsch & McCown Woody plant medium) that contained the growth regulator thidiazuron and differed in their salt and vitamin compositions. One half of each kind of explant was cut prior to being cultured. After five months of culture, embryos had only developed from seeds that were cut previous to sowing. To the best of our knowledge, this is the first time that mature seeds have been used for inducing embryogenesis in grape. A total of 42% of the embryos transferred to tubes for germination regenerated into normal plantlets. The absence of both the GLRaV-1 and GLRaV-3 viruses in all regenerated plants was confirmed by real-time uniplex RT-PCR. So, this protocol can be used for sanitation and also for micropropagation.</p>

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In vitro elimination of Black raspberry necrosis virus from black raspberry (Rubus occidentalis) - Short Communication

Cited by 5 publications

References 23 publications

In vitro thermotherapy-based methods for plant virus eradication

In vitro thermotherapy-based methods for plant virus eradication

Effect of thermotherapy duration, virus type and cultivar interactions on elimination of potato viruses X and S in infected seed stocks

Mature seeds for in vitro sanitation of the Grapevine leafroll associated virus (GLRaV-1 and GLRaV-3) from grape (Vitis vinifera L.)

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