In Vitro Efficacy of Medicinal Plant Material on the Inhibition of Development of Egg of Ascaridia galli

Abstract

| To determine the in vitro efficacy of indigenous plants on the inhibition of development of eggs of Ascaridia galli, a study was carried out in the laboratory of Department of Parasitology from January to May, 2012. Leaves of five plants namely pineapple (Ananas comosus), turmeric (Curcuma domestica), akanda (Calotropis gigantea), garlic (Allium sativum) and custard apple (Annona reticulata) were selected. Two different types of preparation such as fresh juice and dust material were used. Fresh leaves juice at 5%, 10% and 20% concentration and dust of leaves at 5%, 10% and 20% concentration were used for screening. Among the selected plants in all three concentrations of fresh juice of leaves, pineapple was the highest effective plant (86.9%) at 20% concentration followed by pineapple at 10% concentration (84.0%), garlic at 5% concentration (81.8%), turmeric at 20% concentration (78.9%), pineapple at 5% concentration (78.9%), turmeric at 10% concentration (73.9%), garlic at 10% concentration (73.0%). Among the plants in all concentrations of dust of leaves, pineapple at 20% concentration (76.9%) was observed as the best plant followed by akanda at 5% concentration (75.0%) and turmeric at 20% concentration (73.3%). The present study suggests that pineapple, turmeric and garlic leaves are effective and can be used against the development of eggs of A. galli. So, further studies are required to determine the side effects of these plants and determine the recommended doses in poultry.

Ovicidal Effects of Pineapple Peel Juice (Ananas comosus L.) as a Control of Ascaridians in Chicken (Gallus domesticus) In Vitro

“…Pineapple peel was obtained from a honey pineapple seller. Pineapple peel was washed and then processed into fresh juice (Hossain et al, 2015). For the production of fresh juice, the fresh peels were cut into small pieces and aquades was added at 1:1 ratio in a kitchen blender.…”

“…The petri dish placed in a large tray and kept moist. Place it at room temperature for 20 days and observe the development of larvae in eggs on day 5, 10, 15, and 20 (Hossain et al, 2015). The percentage of inhibition of hatchability of worm eggs calculated using the formula: the number of undeveloped worm eggs divided by the total number of eggs before treatment multiplied by 100% (Hossain et al, 2015).…”

The Effects of Allium sativum L., Artemisia absinthium L., Cucurbita pepo L., Coriandrum sativum L., Satureja hortensis L. and Calendula officinalis L. on the Embryogenesis of Ascaris suum Eggs during an In Vitro Experimental Study

“…These herbs have long been used to control parasitic diseases and, in many parts of the world, they are still applied for this purpose. In ethno-veterinary medicine, which draws inspiration from traditional practices, there seems to be a range of plants or plant extracts suitable for treating almost every parasitic disease of livestock [9]. Different in vitro methods, such as egg hatch and larval motility tests, are frequently implied in examining the effects of medicinal plants against nematodes [10].…”