In vitro efficacies of caspofungin or micafungin catheter lock solutions on Candida albicans biofilm growth

Cateau, Estelle; Rodier, Marie‐Hélène; Imbert, Christophe

doi:10.1093/jac/dkn160

Cited by 91 publications

(71 citation statements)

References 11 publications

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“…However, in another study investigating long-term trials of continuous-flow (47). Likewise, in our study and others, C. parapsilosis biofilms exhibited variable CAS and MFG MIC profiles, with MICs ranging from 1 to Ͼ16 mg/liter (19,45,48,49). Several explanations for such variability have been offered.…”

Section: Discussionsupporting

confidence: 66%

Species-Specific and Drug-Specific Differences in Susceptibility of Candida Biofilms to Echinocandins: Characterization of Less Common Bloodstream Isolates

Simitsopoulou

Peshkova

Tasina

et al. 2013

Antimicrob Agents Chemother

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e Candida species other than Candida albicans are increasingly recognized as causes of biofilm-associated infections. This is a comprehensive study that compared the in vitro activities of all three echinocandins against biofilms formed by different common and infrequently identified Candida isolates. We determined the activities of anidulafungin (ANID), caspofungin (CAS), and micafungin (MFG) against planktonic cells and biofilms of bloodstream isolates of C. albicans (15 strains), Candida parapsilosis (6 strains), Candida lusitaniae (16 strains), Candida guilliermondii (5 strains), and Candida krusei (12 strains) by XTT [2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide] assay. Planktonic and biofilm MICs were defined as >50% fungal damage. Planktonic cells of all Candida species were susceptible to the three echinocandins, with MICs of <1 mg/liter. By comparison, differences in the MIC profiles of biofilms in response to echinocandins existed among the Candida species. Thus, C. lusitaniae and C. guilliermondii biofilms were highly recalcitrant to all echinocandins, with MICs of >32 mg/ liter. In contrast, the MICs of all three echinocandins for C. albicans and C. krusei biofilms were relatively low (MICs < 1 mg/ liter). While echinocandins exhibited generally high MICs against C. parapsilosis biofilms, MFG exhibited the lowest MICs against these isolates (4 mg/liter). A paradoxical growth effect was observed with CAS concentrations ranging from 8 to 64 mg/ liter against C. albicans and C. parapsilosis biofilms but not against C. krusei, C. lusitaniae, or C. guilliermondii. While nonalbicans Candida planktonic cells were susceptible to all echinocandins, there were drug-and species-specific differences in susceptibility among biofilms of the various Candida species, with C. lusitaniae and C. guilliermondii exhibiting profiles of high MICs of the three echinocandins.

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Section: Discussionsupporting

confidence: 66%

Species-Specific and Drug-Specific Differences in Susceptibility of Candida Biofilms to Echinocandins: Characterization of Less Common Bloodstream Isolates

Simitsopoulou

Peshkova

Tasina

et al. 2013

Antimicrob Agents Chemother

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“…Similar results were obtained with CAS (87% reduction of biofilm) (P<0.001). The excellent activity of echinocandins, not only during the early stages of biofilm development but also on mature biofilm, may result from the main mechanism of action leading to the inhibition of β-1,3 glucan synthase, which is responsible for forming the glucan polymers in the cell wall [23].…”

Section: Resultsmentioning

confidence: 99%

Effect of antifungals on itraconazole resistant Candida glabrata

Kucharíková¹,

Dijck

Lisalovà³

et al. 2010

Open Life Sciences

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Abstract:In the last decade, infections caused by Candida glabrata have become more serious, particularly due to its decreased susceptibility to azole derivatives and its ability to form biofilm. Here we studied the resistance profile of 42 C. glabrata clinical isolates to different azoles, amphotericin B and echinocandins. This work was also focused on the ability to form biofilm which plays a role in the development of antifungal resistance. The minimal inhibitory concentration testing to antifungal agents was performed according to the CLSI (Clinical and Laboratory Standards Institute) M27-A3 protocol. Quantification of biofilm was done by XTT reduction assay. All C. glabrata clinical isolates were resistant to itraconazole and sixteen also showed resistance to fluconazole. All isolates remained susceptible to voriconazole. Amphotericin B was efficient in a concentration range of 0.125-1 mg/L. The most effective antifungal agents were micafungin and caspofungin with the MIC 100 values of ≤0.0313-0.125 mg/L. Low concentrations of these agents reduced biofilm formation as well. Our results show that resistance of different C. glabrata strains is azole specific and therefore a single azole resistance cannot be assumed to indicate general azole resistance. Echinocandins proved to have very high efficacy against clinical C. glabrata strains including those with ability to form biofilm.© Versita Sp. z o.o.

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“…We also elucidated the effect of pH 5.6 and 7.0 during biofilm development, because adaptation to changes in pH during biofilm formation is crucial not only for its development, but also for survival of microbial communities at colonized host sites (Schmidt et al, 2008) as well as on medical devices (Ramage et al, 2006). The two media tested, YNB and RPMI 1640, have remained favourite choices for in vitro biofilm formation (Biswas & Chaffin, 2005;Cateau et al, 2008;Chandra et al, 2001). YNB medium is less nutrient-rich for growth of planktonic cells than RPMI 1640 medium, which mimics the composition of human fluids (Chandra et al, 2005).…”

Section: Discussionmentioning

confidence: 99%

Detailed comparison of Candida albicans and Candida glabrata biofilms under different conditions and their susceptibility to caspofungin and anidulafungin

Kucharíková

Tournu

Lagrou

et al. 2011

Journal of Medical Microbiology

107

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In vitro efficacies of caspofungin or micafungin catheter lock solutions on Candida albicans biofilm growth

Cited by 91 publications

References 11 publications

Species-Specific and Drug-Specific Differences in Susceptibility of Candida Biofilms to Echinocandins: Characterization of Less Common Bloodstream Isolates

Species-Specific and Drug-Specific Differences in Susceptibility of Candida Biofilms to Echinocandins: Characterization of Less Common Bloodstream Isolates

Effect of antifungals on itraconazole resistant Candida glabrata

Detailed comparison of Candida albicans and Candida glabrata biofilms under different conditions and their susceptibility to caspofungin and anidulafungin

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