Detailed comparison of Candida albicans and Candida glabrata biofilms under different conditions and their susceptibility to caspofungin and anidulafungin

Kucharíková, Soňa; Tournu, Hélène; Lagrou, Katrien; Dijck, Patrick Van; Bujdáková, Helena

doi:10.1099/jmm.0.032037-0

Cited by 104 publications

(80 citation statements)

References 31 publications

(35 reference statements)

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“…Moreover, Ramage et al (2002) found Defg1 sessile cells to be equally resistant to AmB compared to the WT. Such inter-study variations highlight the differences in susceptibilities of Candida biofilms, which can be strongly influenced by different setups, including the type of device, composition and pH of the medium (Kucharíková et al, 2011) and the quantification/susceptibility methods used (Kuhn et al, 2002b).…”

Section: Discussionmentioning

confidence: 99%

Transcription factor Efg1 contributes to the tolerance of Candida albicans biofilms against antifungal agents in vitro and in vivo

Bink

Govaert

Vandenbosch

et al. 2012

Journal of Medical Microbiology

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We investigated the molecular basis of the tolerance of Candida albicans biofilms to antifungals using the miconazole as a model compound, and translated the resulting data to other antifungals. Sessile cells of C. albicans Defg1, lacking the transcription factor Efg1, showed increased susceptibility to miconazole, amphotericin B and caspofungin, whereas these sessile cells were equally resistant to fluconazole. The increased sensitivity to miconazole was, at least, partly due to an increased accumulation of miconazole in the cells as compared to wild-type or reintegrant Defg1(EFG1) sessile cells. By using a rat biofilm model, we further confirmed the role of Efg1 in the tolerance of C. albicans biofilms to miconazole when grown in vivo.

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Section: Discussionmentioning

confidence: 99%

Transcription factor Efg1 contributes to the tolerance of Candida albicans biofilms against antifungal agents in vitro and in vivo

Bink

Govaert

Vandenbosch

et al. 2012

Journal of Medical Microbiology

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“…C. albicans adhesins Als1 and Als3 play an important role and are highly expressed in in vitro (16) as well as in vivo biofi lms (17,18). While the Als1 gene is expressed in the earlier stages of biofi lm formation (19), the Als3 gene is mainly up-regulated in the mature biofi lm (11). However, this is not a rule, as the expression of either gene can be signifi cantly affected by cultivation conditions (11,20).…”

mentioning

confidence: 76%

“…While the Als1 gene is expressed in the earlier stages of biofi lm formation (19), the Als3 gene is mainly up-regulated in the mature biofi lm (11). However, this is not a rule, as the expression of either gene can be signifi cantly affected by cultivation conditions (11,20).…”

mentioning

confidence: 78%

“…Our previous in vitro study (11) confi rmed the effi ciency of anidulafungin and CAS against sessile biofi lm when added at the early stage of formation. Our present study has confirmed that biofilm susceptibility changes in a time-dependent manner.…”

Section: Discussionmentioning

confidence: 99%

“…Therapy can be a problem because biofi lm is usually refractory to conventional therapy due to resistance or reduced uptake of antifungals through the extracellular matrix formed by mature biofi lm (5)(6)(7). Recent in vitro and in vivo studies (8)(9)(10)(11) point to echinocandins caspofungin (CAS) and anidulafungin as effective agents against planktonic cells and biofi lms formed by C. albicans.…”

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confidence: 99%

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Susceptibility To Caspofungin And Fluconazole And Als1/Als3 Gene Expression In Biofilm And Dispersal Cells Of Candida Albicans / Profil Osjetljivosti Na Kaspofungin I Flukonazol I Ekspresija Gena Als1 I Als3 U Stanicama Biofilma Te Planktonskim Stanicama Vrste Candida Albicans

Bujdáková

Kulkova

Černáková

2012

Archives of Industrial Hygiene and Toxicology

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The biofi lm of Candida albicans has been implicated as a source of bloodstream infections. Dispersal cells, as the fi nal biofi lm stage, are responsible for its spread. The aim of this study was to compare the susceptibility of biofi lm and dispersal cells vs. planktonic cells (overnight liquid culture) of C. albicans to caspofungin (CAS) and fl uconazole (FLU) when the drugs were added: i) at the beginning of the experiment; ii) after 1.5 h (adherence stage); iii) after 24 h (early mature biofi lm). The fi ndings were evaluated after 48 h (mature biofi lm) using the XTT reduction assay. Later administration of the drug increased biofi lm sessile minimal inhibitory concentration (SMIC 80 ) of both FLU and CAS from 1 μg mL -1 to over 64 μg mL -1 and from 0.125 μg mL -1 to over 16 μg mL -1 , respectively. Susceptibility of dispersal cells also decreased with time of administration. We also determined the expression of the Als1 and Als3 genes in 48-h sessile biofi lm and dispersal cells of C. albicans SC5314 and compared it to planktonic cells. The expression was normalised to the standard Act1 gene in every condition tested. Quantitative real-time PCR revealed a strong up-regulation of the Als1 gene in the dispersal cells but not in biofi lm and high expression of the Als3 gene in both biofi lm and dispersal cells. High expression of both Als1 and Als3 genes supports the hypothesis that dispersal cells pose a high-risk of infection.

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Anti‐Adhesion Activity of Tannins Isolated from the Mangrove Laguncularia racemosa

Glasenapp

Lucas

Wöltje

et al. 2019

Chemistry & Biodiversity

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In the search of new compounds with biofilm‐inhibiting properties, mangroves with their richness of secondary metabolites can be a valuable resource. Crude methanolic leaf extracts from the mangrove Laguncularia racemosa enriched in phenolic substances cause a reduction in initial cell adhesion of Candida glabrata and Candida albicans, but not on Escherichia coli. LC/MS‐guided fractionation of the phenolic compounds resulted in 19 fractions, of which ten were analyzed for their bioactivity against cell adhesion. Effects on cell adhesion and planktonic growth of Escherichia coli, Candida glabrata and Candida albicans were measured in 96‐well microtiter plates in the presence of 0.2 mg ml−1 of the isolated fractions. Two fractions caused a reduction of cell adhesion of Candida albicans. These fractions containing bioactive compounds were analyzed by LC/MS and NMR spectroscopy. Casuarinin and digalloyl‐hexahydroxydiphenoyl‐glucose were identified in the active fractions, in addition to three signals of ellagitannins. These results indicate a specific mode of action of hydrolysable tannins against cell adhesion of Candida albicans, which needs to be further analyzed.

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Detailed comparison of Candida albicans and Candida glabrata biofilms under different conditions and their susceptibility to caspofungin and anidulafungin

Cited by 104 publications

References 31 publications

Transcription factor Efg1 contributes to the tolerance of Candida albicans biofilms against antifungal agents in vitro and in vivo

Transcription factor Efg1 contributes to the tolerance of Candida albicans biofilms against antifungal agents in vitro and in vivo

Susceptibility To Caspofungin And Fluconazole And Als1/Als3 Gene Expression In Biofilm And Dispersal Cells Of Candida Albicans / Profil Osjetljivosti Na Kaspofungin I Flukonazol I Ekspresija Gena Als1 I Als3 U Stanicama Biofilma Te Planktonskim Stanicama Vrste Candida Albicans

Anti‐Adhesion Activity of Tannins Isolated from the Mangrove Laguncularia racemosa

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