In vitro effects of vitamins C and E on adipocyte function and redox status in obesity

Imessaoudene, Asmahan; Merzouk, Amel Z.; Guermouche, Baya; Merzouk, Hafida; Merzouk, Sid Ahmed

doi:10.1016/j.phanu.2022.100315

Cited by 4 publications

(3 citation statements)

References 46 publications

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“…The treatment of ZnO-NPs and ascorbic acid assisted in reducing the anti-oxidant and anti-fibrotic effects of ascorbic acid as reflected by some blood hemolysis in central vein and moderately organized hepatocytes in cords. In mice, ascorbic acid improves liver fibrosis by increasing anti-oxidant enzyme activity and lowering pro-oxidant effects (Imessaoudene et al, 2022;Sae-Khow et al, 2022).…”

Section: Discussionmentioning

confidence: 99%

Possible Effect of Ascorbic Acid against Zinc Oxide Nanoparticles Induced Hepatotoxicity in Swiss Albino Mice

2023

IJAB

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Zinc oxide nanoparticles (ZnO-NPs) has long been used in cosmetics and paints without knowing its possible side effects in human beings. This study was conducted to find whether ZnO-NPs toxicity can be reversed by the administration of ascorbic acid in mouse. Swiss albino mice were divided into 4 groups; control group, ascorbic acid (100mg/kg), ZnO-NPs (30mg/kg) and ascorbic acid + ZnO-NPs for 21 alternate days. After that mice were decapitated and liver was preserved for histopathological analysis. Ascorbic acid groups showed the normal liver structure while ZnO-NPs group exhibited inflammation and necrosis of hepatocytes. The combination of ascorbic acid+ZnO-NPs showed reduced inflammation and necrosis after 28 days of treatment. Ascorbic acid showed hepatoprotective effect against liver fibrosis by increasing the activity of anti-oxidant enzyme and lowering of pro-oxidant effects in mice. This study concluded hepatoprotective effect of ascorbic acid against ZnO-NPs induced liver damage in mice.

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Section: Discussionmentioning

confidence: 99%

Possible Effect of Ascorbic Acid against Zinc Oxide Nanoparticles Induced Hepatotoxicity in Swiss Albino Mice

2023

IJAB

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“…The volume of control wells to 100 μL was adjusted with Collagenase Assay Buffer. For reagent background control, 100 μL of Collagenase Assay Buffer was added [10,11]. To determine collagenase activity (U/Ml), equation 1 was used:…”

Section: Collagenase Inhibition Activitymentioning

confidence: 99%

Study on hydrogel eye mask with Centella asiatica L and Aloe vera L extract

Dewi,

Surini

2024

J Adv Pharm Educ Res

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“…It is an essential molecule that plays a vital role in many biological processes, including immune response and antioxidant activity. Vitamin C plays significant roles in amino acid and collagen synthesis, cholesterol metabolism, and DNA protection by acting as a free radical scavenger (12). It is also involved in many biological activities, including enzymatic reactions catalyzed by dioxygenases that use Fe 2+ and 2-oxoglutarate as co-substrates (13).…”

Section: Introductionmentioning

confidence: 99%

In Vitro Culture of Ovine Spermatogonial Stem Cells: Effects of Grape Seed Extracts and Vitamin C

Zolfaghari,

Zandi,

Sanjabi

et al. 2023

Gene Cell Tissue

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Background: Spermatogonial stem cells (SSCs) transmit genetic information to the next generation. For the successful transplantation of these cells, the culture medium of these cells must be optimized. Objectives: This study investigated the natural antioxidant of grape seed extract (GSE) in comparison to vitamin C in the culture medium of SSCs. Methods: A Soxhlet extractor was used to prepare ethanolic and acetonic extracts, and the Clevenger apparatus was used to prepare aqueous extracts of grape seeds. Then, their antioxidant capacities were determined using the DPPH method. The SSCs were extracted from lamb testicular tissue by a two-step digestion method, and different concentrations of GSE and vitamin C were investigated for survival, colony formation, and expression of apoptotic-related genes. Results: The results showed that the acetonic extract in the concentration of 400 µg/mL showed about 90% antioxidant properties based on the DPPH test; nevertheless, the aqueous and ethanolic extracts had only 50% of their antioxidant properties at this concentration. The acetonic extracts significantly decreased the viability of SSCs without any colony formation when used in a culture medium. The highest survival rate was obtained from the ethanolic extracts of grape seeds at the concentration of 50 µg/mL, and a significant difference was obtained with ethanolic extracts (100 to 1000 µg/mL) (P < 0.05). The viability of SSCs treated with vitamin C (50 µg/mL) was significantly higher (P < 0.05) than the control and 5 - 25 µg/mL of vitamin C groups. Conclusions: Aqueous, ethanolic, and acetonic extracts of grape seeds should not be used in the culture of SSCs. However, the use of 25 - 50 µg/mL of vitamin C is recommended in the culture of SSCs.

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In vitro effects of vitamins C and E on adipocyte function and redox status in obesity

Cited by 4 publications

References 46 publications

Possible Effect of Ascorbic Acid against Zinc Oxide Nanoparticles Induced Hepatotoxicity in Swiss Albino Mice

Possible Effect of Ascorbic Acid against Zinc Oxide Nanoparticles Induced Hepatotoxicity in Swiss Albino Mice

Study on hydrogel eye mask with Centella asiatica L and Aloe vera L extract

In Vitro Culture of Ovine Spermatogonial Stem Cells: Effects of Grape Seed Extracts and Vitamin C

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