In Vitro Effects of Three Blood Derivatives on Human Corneal Epithelial Cells

Freire, Vanesa; Andollo, Noelia; Etxebarría, Jaime; Durán, Jorge; Morales, María–Celia

doi:10.1167/iovs.11-7340

Cited by 78 publications

(48 citation statements)

References 53 publications

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“…A growing body of studies has demonstrated that EGF can accelerate reepithelialization by increasing the proliferation and cell migration of keratinocytes in acute wound [23–27]. V. Frelrd et al [20] demonstrated the effect of EGF on fibroblasts proliferation, and our results confirmed the former result. In our study, EGF promoted the proliferation of fibroblasts.…”

Section: Discussionsupporting

confidence: 88%

The Effects of Cytokines in Adipose Stem Cell-Conditioned Medium on the Migration and Proliferation of Skin FibroblastsIn Vitro

Zhao

Liu

et al. 2013

BioMed Research International

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Although adipose stem cell-conditioned medium (ASC-CM) has demonstrated the effect of promoting the cutaneous wound healing, the mechanism for this response on the effector cells (e.g., dermal fibroblasts) during the process remains to be determined. In this study, we aim to investigate the types and contents of cytokines in ASC-CM and the effects of some kinds of common cytokines in ASC-CM, such as EGF, PDGF-AA, VEGF, and bFGF, on dermal fibroblasts proliferation and migration in wound healing process. Results showed that these four cytokines had high concentrations in ASC-CM. The migration of skin fibroblasts could be significantly stimulated by VEGF, bFGF, and PDGF-AA, and the proliferation could be significantly stimulated by bFGF and EGF in ASC-CM. Additionally, ASC-CM had more obvious promoting effect on fibroblasts proliferation and migration than single cytokine. These observations suggested that ASC-CM played an important role in the cutaneous injury partly by the synergistic actions of several cytokines in promoting dermal fibroblasts proliferation and migration, and ASC-CM was more adaptive than each single cytokine to be applied in promoting the wound healing.

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Section: Discussionsupporting

confidence: 88%

The Effects of Cytokines in Adipose Stem Cell-Conditioned Medium on the Migration and Proliferation of Skin FibroblastsIn Vitro

Zhao

Liu

et al. 2013

BioMed Research International

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“…Platelet-derived products have shown an important role in the treatment of dry eye syndrome, dormant ulcers (epithelial defects of the cornea that fail to heal), ocular surface syndrome after Laser In Situ Keratomileusis (LASIK), and for surface reconstruction after corneal perforation associated also with amniotic membrane transplantation [61,[112][113][114][115]. Furthermore, platelet derivatives appear to show therapeutic effects in some diseases such as diabetic keratitis, keratocongiuntivite sicca, traumatic post-infectious and vernal ulcers that lead to a condition known as persistent epithelial defects [116].…”

Section: Ophthalmologymentioning

confidence: 99%

Platelet Derivatives in Regenerative Medicine: An Update

Pascale¹,

Sommese²,

Casamassimi³

et al. 2015

Transfusion Medicine Reviews

169

149

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“…This secretion is intense in the first hour and platelets continue synthesizing more cytokines and growth factors from their mRNA reserves for at least another 7 days [1]. More than 800 different proteins are secreted into the surrounding media [1,2], having a paracrine effect on different cell types: myocytes [3], tendon cells [3-7], mesenchymal stem cells from different origins [8-11], chondrocytes [12-14], osteoblasts [3,15,16], fibroblasts [17-19] and endothelial cells [20]. Cell proliferation, angiogenesis and cell migration are stimulated, resulting in tissue regeneration.…”

Section: Introductionmentioning

confidence: 99%

Platelet-rich plasma preparation for regenerative medicine: optimization and quantification of cytokines and growth factors

Amable¹,

Carias²,

Teixeira³

et al. 2013

Stem Cell Res Ther

548

422

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IntroductionPlatelet-rich plasma (PRP) is nowadays widely applied in different clinical scenarios, such as orthopedics, ophthalmology and healing therapies, as a growth factor pool for improving tissue regeneration. Studies into its clinical efficiency are not conclusive and one of the main reasons for this is that different PRP preparations are used, eliciting different responses that cannot be compared. Platelet quantification and the growth factor content definition must be defined in order to understand molecular mechanisms behind PRP regenerative strength. Standardization of PRP preparations is thus urgently needed.MethodsPRP was prepared by centrifugation varying the relative centrifugal force, temperature, and time. Having quantified platelet recovery and yield, the two-step procedure that rendered the highest output was chosen and further analyzed. Cytokine content was determined in different fractions obtained throughout the whole centrifugation procedure.ResultsOur method showed reproducibility when applied to different blood donors. We recovered 46.9 to 69.5% of total initial platelets and the procedure resulted in a 5.4-fold to 7.3-fold increase in platelet concentration (1.4 × 106 to 1.9 × 106 platelets/μl). Platelets were highly purified, because only <0.3% from the initial red blood cells and leukocytes was present in the final PRP preparation. We also quantified growth factors, cytokines and chemokines secreted by the concentrated platelets after activation with calcium and calcium/thrombin. High concentrations of platelet-derived growth factor, endothelial growth factor and transforming growth factor (TGF) were secreted, together with the anti-inflammatory and proinflammatory cytokines interleukin (IL)-4, IL-8, IL-13, IL-17, tumor necrosis factor (TNF)-α and interferon (IFN)-α. No cytokines were secreted before platelet activation. TGF-β3 and IFNγ were not detected in any studied fraction. Clots obtained after platelet coagulation retained a high concentration of several growth factors, including platelet-derived growth factor and TGF.ConclusionsOur study resulted in a consistent PRP preparation method that yielded a cytokine and growth factor pool from different donors with high reproducibility. These findings support the use of PRP in therapies aiming for tissue regeneration, and its content characterization will allow us to understand and improve the clinical outcomes.

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In Vitro Effects of Three Blood Derivatives on Human Corneal Epithelial Cells

Cited by 78 publications

References 53 publications

The Effects of Cytokines in Adipose Stem Cell-Conditioned Medium on the Migration and Proliferation of Skin FibroblastsIn Vitro

The Effects of Cytokines in Adipose Stem Cell-Conditioned Medium on the Migration and Proliferation of Skin FibroblastsIn Vitro

Platelet Derivatives in Regenerative Medicine: An Update

Platelet-rich plasma preparation for regenerative medicine: optimization and quantification of cytokines and growth factors

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