The Effects of Cytokines in Adipose Stem Cell-Conditioned Medium on the Migration and Proliferation of Skin FibroblastsIn Vitro

Abstract

Although adipose stem cell-conditioned medium (ASC-CM) has demonstrated the effect of promoting the cutaneous wound healing, the mechanism for this response on the effector cells (e.g., dermal fibroblasts) during the process remains to be determined. In this study, we aim to investigate the types and contents of cytokines in ASC-CM and the effects of some kinds of common cytokines in ASC-CM, such as EGF, PDGF-AA, VEGF, and bFGF, on dermal fibroblasts proliferation and migration in wound healing process. Results showed that these four cytokines had high concentrations in ASC-CM. The migration of skin fibroblasts could be significantly stimulated by VEGF, bFGF, and PDGF-AA, and the proliferation could be significantly stimulated by bFGF and EGF in ASC-CM. Additionally, ASC-CM had more obvious promoting effect on fibroblasts proliferation and migration than single cytokine. These observations suggested that ASC-CM played an important role in the cutaneous injury partly by the synergistic actions of several cytokines in promoting dermal fibroblasts proliferation and migration, and ASC-CM was more adaptive than each single cytokine to be applied in promoting the wound healing.

In vitro comparative study of human mesenchymal stromal cells from dermis and adipose tissue for application in skin wound healing

“…During the wound healing process, skin-resident cells are influenced by growth factors and other proteins secreted by MSCs, such as FGF 2 , EGF, KGF, and TGF-β (Zhao, Hu, Liu, Gong, & Chen, 2013;Zhou et al, 2013). In this sense, the conditioned medium of MSCs is a powerful source of these factors (Zhao et al, 2013). In the present study, the paracrine effect of DSCs and ASCs on cutaneous wound healing was evaluated by their action on human dermal fibroblasts and keratinocytes.…”

“…Divergences in the methodology between studies could explain the discrepancy in the findings. During the wound healing process, skin-resident cells are influenced by growth factors and other proteins secreted by MSCs, such as FGF 2 , EGF, KGF, and TGF-β (Zhao, Hu, Liu, Gong, & Chen, 2013;Zhou et al, 2013). In this sense, the conditioned medium of MSCs is a powerful source of these factors (Zhao et al, 2013).…”

Corrigendum to “The Effects of Cytokines in Adipose Stem Cell-Conditioned Medium on the Migration and Proliferation of Skin Fibroblasts In Vitro”

“…In the article titled “The Effects of Cytokines in Adipose Stem Cell-Conditioned Medium on the Migration and Proliferation of Skin Fibroblasts In Vitro ” [1], the pictures used in Figure 1(d) are the same as the pictures used in Figures 2(a1)–2(a4) of another article by the same authors, Li et al, “Side-by-Side Comparison of the Biological Characteristics of Human Umbilical Cord and Adipose Tissue-Derived Mesenchymal Stem Cells,” BioMed Research International, vol. 2013, Article ID 438243, 12 pages, 2013. doi: 10.1155/2013/438243.…”

Secretome of Adipose Tissue-Derived Stem Cells (ASCs) as a Novel Trend in Chronic Non-Healing Wounds: An Overview of Experimental In Vitro and In Vivo Studies and Methodological Variables

“…CM was then applied to HDFs at varying dilution folds (0%, 10%, 50% and 100%) in DMEM/F12 with 2% FBS. Using the same cellular model, Zhao et al [57] showed that CM collected from ASCs (at passage 3-7) cultured for an additional 48 h in serum-free DMEM/F12, at 50% dilution, promoted the proliferation and migration of HDFs. To analyze the effects of ASC-CM on ASCs, HDFs, normal primary human umbilical vein endothelial cells (HUVECs), and human keratinocytes, Kober et al [58] used CM collected from stem cell-enriched stromal vascular fraction (SVF) cultured in either FBS-free or FBS-supplemented DMEM for 20 h. ASC-CM was used at a 1:1 dilution on cell cultures.…”