In Vitro Effects of the Reduced Form of Coenzyme Q10 on Secretion Levels of TNF-α and Chemokines in Response to LPS in the Human Monocytic Cell Line THP-1

Abstract

Summary Ubiquinol-10 (QH2), the reduced form of Coenzyme Q10 (CoQ10) serves as a potent antioxidant of lipid membranes. Because many antioxidants reveal potent anti-inflammatory effects, the influence of QH2 on lipopolysaccharide (LPS)-induced pro-inflammatory cytokines and chemokines were determined in the human monocytic cell line THP-1. Stimulation of cells with LPS resulted in a distinct release of Tumour necrosis factor-alpha (TNF-α), Macrophage inflammatory protein-1 alpha (MIP-1α), Regulated upon activation, normal T cell expressed and secreted (RANTES) and Monocyte chemotattractant protein-1 (MCP-1). The LPS-induced responses were significantly decreased by pre-incubation of cells with QH2 to 60.27 ± 9.3% (p = 0.0009), 48.13 ± 6.93% (p = 0.0007) and 74.36 ± 7.25% (p = 0.008) for TNF-α, MIP-1α and RANTES, respectively. In conclusion, our results indicate anti-inflammatory effects of the reduced form of CoQ10 on various proinflammatory cytokines and chemokines in vitro.

Coenzyme Q10 supplementation downregulates the increase of monocytes expressing toll-like receptor 4 in response to 6-day intensive training in kendo athletes

“…CoQ10 acts as an antioxidant in mitochondria and lipid membranes by scavenging ROS, either directly or in conjunction with vitamin E (␣-tocopherol) (Battinoa et al 1991;Zhou et al 1999). Moreover, exposure to CoQ10 downregulates production of the inflammatory cytokine tumor necrosis factor ␣ (TNF-␣) in response to lipopolysaccharide (LPS) in monocytes (Schmelzer et al 2009). Under conditions of high oxidative stress, the ability of cells to eliminate ROS is exhausted.…”

Coenzyme Q10 supplementation lowers hepatic oxidative stress and inflammation associated with diet-induced obesity in mice

“…CoQ10 is also involved in oxidative phosphorylation as an electron transporter and is therefore involved in energy homeostasis [17][18][19][20]. Data obtained in vitro even suggest its antiinflammatory potency [21][22][23][24][25]. However, the effect of CoQ10 given orally remains only poorly explored in the context of obesity and diabetes.…”

MicroRNA-146a and hemopoietic disorders

“…Moreover, other reports describe that miR-146 is also induced after stimulation by TNF-a, IL-1b and TLRs [27,34,35]. It inhibits innate immune responses by targeting several cytoplasmic components of TLR signaling in a negative feedback pathway.…”

“…Preincubation of THP-1 cells with ubiquinol-10 can reduce the LPS-induced expression level of miR-146a. Ubiquinol-10 lowers the LPS-stimulated release of some proinflammatory cytokines and chemokines relevant in inflammatory processes [27]. Diazoxide potentiates mesenchymal stem cell (MSC) survival via NF-jB-dependent miR-146a expression by targeting Fas in young male Fischer-344 rat model.…”