In vitro effects of Tabernaemontana citrifolia extracts on Haemonchus contortus

Marie-Magdeleine, Carine; Mahieu, Maurice; d'Alexis, Séverine; Philibert, Lucien; Archimède, Harry

doi:10.1016/j.rvsc.2010.01.002

Cited by 21 publications

(15 citation statements)

References 21 publications

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“…From the results, the different parts of T. citrifolia showed significant effects with differences depending on the parasitic stage such as efficacies on the larval development stage for fruits extract. These results suggest that T. citrifolia will be to possess anthelmintic activity against H. contortus [14]. …”

Section: Tabernaemontana Citrifolia Linnmentioning

confidence: 81%

A Review on Tabernaemontana spp.: Multipotential Medicinal Plant

Athipornchai

2018

Asian J Pharm Clin Res

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Plants in the genus Tabernaemontana have been using in Thai and Chinese traditional medicine for the treatment several diseases. The great majority constituents of Tabernaemontana species have already been subjected to isolation and identification of monoterpene indole alkaloids present in their several parts. Many of monoterpene indole alkaloids exhibited a wide array of several activities. The biogenesis, classification, and biological activities of these alkaloids which found in Tabernaemontana plants were discussed in this review and its brings the research up-to-date on the bioactive compounds produced by Tabernaemontana species, directly or indirectly related to human health.

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Section: Tabernaemontana Citrifolia Linnmentioning

confidence: 81%

A Review on Tabernaemontana spp.: Multipotential Medicinal Plant

Athipornchai

2018

Asian J Pharm Clin Res

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“…The detrimental effects that H. contortus nematodes have on the development of goats and sheep have led to numerous studies examining the use of plant extracts as nematode control agents (ALONSO-DÍAZ et al, 2008;MARIE-MAGDELEINE et al, 2010;HERNÁNDEZ-VILLEGAS et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

Anthelmintic activity of Leucaena leucocephala protein extracts on Haemonchus contortus

Soares

Araújo

Lopes

et al. 2015

Rev. Bras. Parasitol. Vet.

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The objective of this study was to evaluate the effects of protein extracts obtained from the plant Leucaena leucocephala on the nematode parasite Haemonchus contortus. The seeds, shell and cotyledon of L. leucocephala were separated and their proteins extracted using a sodium phosphate buffer, and named as TE (total seed extract), SE (shell extract) and CE (cotyledon extract). Soluble protein content, protease, protease inhibitory and chitinase activity assays were performed. Exsheathment inhibition of H. contortus larvae were performed at concentrations of 0.6 mg mL . The effective concentration for 50% hatching inhibition (EC 50 ) was estimated by probit. Different proportions of soluble proteins, protease and chitinase were found in TE and CE. Protease inhibitory activity was detected in all extracts. The EC 50 of the CE and TE extracts were 0.48 and 0.33 mg mL -1 , respectively. No ovicidal effects on H. contortus were detected in SE extracts, and none of the protein extracts demonstrated larvicidal effects on H. contortus. We therefore conclude that protein extracts of L. leucocephala had a detrimental effect on nematode eggs, which can be correlated with the high protease and chitinase activity of these extracts.Keywords: Plant proteins, protease, protease inhibitor, chitinase. ResumoO objetivo deste estudo foi avaliar a atividade anti-helmíntica de extratos proteicos de leucena (Leucaena leucocephala) sobre Haemonchus contortus. As sementes, as cascas e os cotilédones foram moídos separadamente e as proteínas extraídas com tampão fosfato de sódio e denominados: TE (extrato total), SE (extrato casca) e CE (extrato cotilédone). O teor de proteínas, atividade proteolítica, inibitória de protease e quitinolítica dos extratos foram verificados, além da ação sobre a eclosão de ovos e desembainhamento larvar de H. contortus. A concentração efetiva para inibição de 50% da eclosão dos ovos (EC 50 ) foi calculada através do probit. Foi demonstrado que TE e CE possuem, em diferentes proporções, proteínas solúveis, protease e quitinase. Atividade inibitória de protease foi encontrada em todos os extratos. A EC 50 dos extratos CE e TE foram 0,48 e 0,33 mg de proteína mL -1 , respectivamente. O extrato SE não apresentou atividade sobre a eclosão dos ovos. Os extratos proteicos não apresentaram efeito larvicida sobre H. contortus. Conclui-se que a ação de extratos proteicos de L. leucocephala afetam negativamente a eclodibilidade dos ovos, correlacionando-se com a alta atividade de protease e quitinase dos extratos testados.Palavras-chaves: Proteínas vegetais, protease, inibidor de protease, quitinase.

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“…Eggs, larvae and adults Inhibition of hatching, development and motility of larvae and adults [68] Melia azedarach…”

Section: H Contortusmentioning

confidence: 99%

Plants and their medicinal potential for controling gastrointestinal nematodes in ruminants

Borges¹,

Borges²

2016

Nematoda

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HIGHLIGHTS• Plants with anthelmintic activity may have potential sources of chemical substances that can be used to control gastrointestinal nematodes in ruminants.• The main difficulty of the research with herbal medicines is the lack of in vitro and in vivo correlation with phytotherapic substances activities.• Better understanding the interactions between metabolites and the use of appropriate methodologies to evaluate phytotherapics, may contribute to in vivo results. ABSTRACT:Gastrointestinal nematodes (GINs) is one of the main sanitary problems of ruminant herds, causing large economic losses to breeders. Chemical control continues to be the main measure used to combat GINs, but as resistant parasite populations spread, the efficiency of this method is becoming impaired. In light of this situation, alternative ways to control verminosis are gaining importance. Among these, phytotherapy has attracted particular attention. This review presents the current status of research on phytotherapeutic agents, their difficulties and perspectives. Plants with nematicidal activity are a potential source of chemical substances, including new molecules, that can be used to control GINs in ruminants. The principal difficulty of research into phytotherapic substances is to identify the bioactive compounds, because in many cases in vitro tests have shown promising results, but in vivo test results have been disappointing. Among the causes is host-drug interaction. Therefore, more careful choice of plants with anthelmintic potential by adoption of a standardized system to analyze the results of in vitro testing, combined with better understanding of the synergistic relations among metabolites and the adjustment of methods for in vivo testing, can make phytotherapy for control of GINs more effective.

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In vitro effects of Tabernaemontana citrifolia extracts on Haemonchus contortus

Cited by 21 publications

References 21 publications

A Review on Tabernaemontana spp.: Multipotential Medicinal Plant

A Review on Tabernaemontana spp.: Multipotential Medicinal Plant

Anthelmintic activity of Leucaena leucocephala protein extracts on Haemonchus contortus

Plants and their medicinal potential for controling gastrointestinal nematodes in ruminants

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