In vitro effects of sterculic acid on lipid biosynthesis inRhodococcus opacusstrain PD630 and isolation of mutants defective in fatty acid desaturation

Wältermann, Marc; Steinbüchel, Alexander

doi:10.1111/j.1574-6968.2000.tb09260.x

Cited by 13 publications

(5 citation statements)

References 25 publications

(26 reference statements)

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“…Sterculic acidis is an inhibitor of the stearoyl-CoA desaturase, when added it to the medium, it could inhibit the saturated fatty acids synthesis of more than 16 carbon atoms and of odd-numbered fatty acids (Jeffcoat and Pollard, 1977). There was an almost complete growth suppression of Rhodococcus opacus PD630 when added 0.25 mM sterculic acid in the medium and the total fatty acid content was decreased to 46.7% (w/w), while the content of total fatty acid was 76% when did not added sterculic acid in the medium (Wältermann and Steinbüchel, 2000). The ratio of stearic to oleic acid showed a dose response effect, with an increase in cyclopropene fatty acid dose, stearic acid content also increased with a corresponding reduction in oleic acid when subjected to Candida sp.…”

Section: Effect Of Sterculic Acid Methyl Ester On Cell Growth and Lipmentioning

confidence: 83%

Cocoa-Butter-Equivalent Production from Yarrowia lipolytica by Optimization of Fermentation Technology

Zhao¹,

Li²,

Xiong³

et al. 2016

American Journal of Biochemistry and Biotechnology

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In order to obtain the optimal control point for single-cell lipid production with fatty acid profile similar to cocoa butter, this study explored the effects of carbon source, nitrogen source, temperature, sterculic acid methyl ester, cobalt on cell growth and lipid accumulation of Yarrowia lipolytica. Glycerol and ammonium tartrate-yeast extract were chosen as carbon source and nitrogen source for production of cocoa butter equivalent. Gradual increase in temperature (20-35°C) resulted in growth reduction, while increased in lipid content per Cell Dry Weight (CDW) (10.13 to 19.7%, w/w) and Saturated Fatty Acids (SFA). Our results suggests that the optimum conditions for Y. lipolytica to synthesize cocoa butter equivalent was 30°C with 0.6 mg L −1 of CoCl 2 ·6H 2 O or 0.03 mL L −1 sterculic acid methyl ester in the medium with glycerol and ammonium tartrate-yeast extract as carbon source and nitrogen source.

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Section: Effect Of Sterculic Acid Methyl Ester On Cell Growth and Lipmentioning

confidence: 83%

Cocoa-Butter-Equivalent Production from Yarrowia lipolytica by Optimization of Fermentation Technology

Zhao¹,

Li²,

Xiong³

et al. 2016

American Journal of Biochemistry and Biotechnology

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“…In addition, DAG can also be derived from PL by the action of phospholipase C 7,126 The distribution of acyl groups on the hydroxyl groups of the glycerol backbone is nonrandom, as has been demonstrated for R. opacus PD630. 6,127 The shorter and saturated fatty acids were esterified to the hydroxyl group at position 2, whereas unsaturated fatty acids were preferentially found at position 3. This distribution in bacterial TAG is different from the TAG of mammals and plants, where the longer unsaturated fatty acids are found at position sn-2.…”

Section: The Case Of Bacteriamentioning

confidence: 99%

Accumulation of High-Value Lipids in Single-Cell Microorganisms: A Mechanistic Approach and Future Perspectives

Garay

Boundy‐Mills

German

2014

J. Agric. Food Chem.

134

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In recent years attention has been focused on the utilization of microorganisms as alternatives for industrial and nutritional applications. Considerable research has been devoted to techniques for growth, extraction, and purification of high-value lipids for their use as biofuels and biosurfactants as well as high-value metabolites for nutrition and health. These successes argue that the elucidation of the mechanisms underlying the microbial biosynthesis of such molecules, which are far from being completely understood, now will yield spectacular opportunities for industrial scale biomolecular production. There are important additional questions to be solved to optimize the processing strategies to take advantage of the assets of microbial lipids. The present review describes the current state of knowledge regarding lipid biosynthesis, accumulation, and transport mechanisms present in single-cell organisms, specifically yeasts, microalgae, bacteria, and archaea. Similarities and differences in biochemical pathways and strategies of different microorganisms provide a diverse toolset to the expansion of biotechnologies for lipid production. This paper is intended to inspire a generation of lipid scientists to insights that will drive the biotechnologies of microbial production as uniquely enabling players of lipid biotherapeutics, biofuels, biomaterials, and other opportunity areas into the 21st century.

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“…Sterculic acid is one kind of cyclopropene fatty acid, which can be used as an inhibitor of the stearoylCoA desaturase system to inhibit the synthesis of monoenic fatty acids (James et al, 1968;Jeffcoat and Pollard, 1968;Reiser and Raju, 1964;Wältermann and Steinbüchel, 2000). Sterculic acid also can inhibit mammary carcinoma growth in rats (Khoo et al, 1991).…”

Section: Introductionmentioning

confidence: 99%

Enzymatic production of sterculic acid from the novel Phoenix tree seed oil: Optimization and kinetic study

Hou

Sun

2017

Grasas y Aceites

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SUMMARY:Phoenix tree (Firmiana simplex) seed oil is a novel oil which is rich in sterculic acid. Sterculic acid, a cyclopropene fatty acid, can be used as the inhibitor of the stearoyl-CoA desaturase system and mammary carcinomas growth. In this work, Lipozyme TLIM-catalyzed hydrolysis of the novel Phoenix tree seed oil was used to prepare sterculic acid. High temperature GC-FID and the degree of hydrolysis (DH) were used to monitor the reaction progress. Effects of reaction variables on the hydrolysis were evaluated and optimized using response surface methodology. Results showed that sterculic acid can be successfully prepared from the novel seed oil, and the effect of reaction variables on the hydrolysis decreased in the order of reaction time > enzyme load > temperature. RESUMEN: Producción enzimática de ácido estercúlico a partir del nuevo aceite de semillas del árbol fenix: optimización y estudio cinético. El aceite de semilla del árbol fenix (Firmiana simplex) es un nuevo tipo de aceite rico en ácido estercúlico. Este es un ácido graso ciclopropeno, que se puede utilizar como inhibidor del sistema de estearoyl-CoA desaturasa y del crecimiento de los carcinomas mamarios. En este trabajo, se utilizó la hidrólisis catalizada por Lipozyme TLIM del nuevo aceite de semilla de árbol fenix para preparar ácido estercúlico. Se utilizaron GC-FID a alta temperatura y el grado de hidrólisis (DH) para monitorizar el progreso de la reacción. Los efectos de las variables de reacción de hidrólisis se evaluaron y optimizaron utilizando la metodología de superficie de respuesta. Los resultados mostraron que el ácido estercúlico puede prepararse con éxito a partir del nuevo aceite de semilla, y el efecto de las variables de reacción en la hidrólisis disminuyó en el orden de tiempo de reacción> carga enzimática> temperatura. Se pueden obtener altos rendimientos de ácidos grasos (DH, 98,2 ± 0,8%) en condiciones optimizadas (45 ºC, relación masa de agua a aceite 10: 1, carga enzimática 10% y 18 h). La ecuación de Arrhenius para la hidrólisis fue LnV 0 = 9,12 − 4721/T. La energía de activación fue 39,25KJ/mol. Los valores cinéticos de Vmax, K/m fueron 0,232 mol/(L • min) y 0,084 mol/L, respectivamente. Citation/Cómo citar este artículo: Hou X, Sun S. 2017. Enzymatic production of sterculic acid from the novel Phoenix tree seed oil: Optimization and kinetic study. Grasas Aceites 68 (2), e197. http://dx

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In vitro effects of sterculic acid on lipid biosynthesis inRhodococcus opacusstrain PD630 and isolation of mutants defective in fatty acid desaturation

Cited by 13 publications

References 25 publications

Cocoa-Butter-Equivalent Production from <i>Yarrowia lipolytica</i> by Optimization of Fermentation Technology

Cocoa-Butter-Equivalent Production from <i>Yarrowia lipolytica</i> by Optimization of Fermentation Technology

Accumulation of High-Value Lipids in Single-Cell Microorganisms: A Mechanistic Approach and Future Perspectives

Enzymatic production of sterculic acid from the novel Phoenix tree seed oil: Optimization and kinetic study

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