In vitro effects of oestrogen on 5α reduction of testosterone in hormone-dependent rat mammary carcinomata

Miller, William R.

doi:10.1038/bjc.1976.74

Br J Cancer

1976

DOI: 10.1038/bjc.1976.74

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In vitro effects of oestrogen on 5α reduction of testosterone in hormone-dependent rat mammary carcinomata

William R. Miller¹

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Cited by 5 publications

(7 citation statements)

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“…In vitro addition of oestradiol 17/? to incubations of hormone-dependent rat mammary carcinomas is associated with an inhibition of 5a reduction of testosterone (Miller, 1976a). The aim of the present study was to determine if in vivo hormone manipulation was associated with similar changes.…”

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Hormonal status and testosterone metabolism of DMBA-induced rat mammary carcinomas

Wr¹

1976

Br J Cancer

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“…Furthermore, the decreased metabolism and conversion of testosterone to 5ac androstanediol following in vivo administration of oestrogen may be reproduced in vitro by addition of oestradiol to incubations of hormone-dependent rat mammary carcinomas (Miller, 1976a).…”

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confidence: 98%

Hormonal status and testosterone metabolism of DMBA-induced rat mammary carcinomas

Wr¹

1976

Br J Cancer

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Summary.-A rat mammary carcinoma induced by [7][8][9][10][11][12] (Miller, 1976a) and in vivo (Miller, 1976b,c;Buchan et al, 1976). The Tumour -steroid metaboli8M.-A portion of each tumour (0-5 g) was finely sliced in KrebsRinger phosphate buffer, pH 7-4 (5 ml).

…”

mentioning

confidence: 99%

“…(DMBA) have been shown to metabolize testosterone, primarily by 5cx reduction, an activity which may be influenced by hormones both in vitro (Miller, 1976a) and in vivo (Miller, 1976b,c;Buchan et al, 1976). The Tumour -steroid metaboli8M.-A portion of each tumour (0-5 g) was finely sliced in KrebsRinger phosphate buffer, pH 7-4 (5 ml).…”

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Effects of serial passage on the endocrine response and steroid metabolism of a rat mammary carcinoma

Miller¹

1980

Br J Cancer

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“…In previous studies, we have investigated the metabolism of testosterone by a predominantly ovary-dependent DMBA-induced mammary tumour, and have shown it to be sensitive to levels of other hormones present in vitro (Miller, 1976a), or in vivo (Miller, 1976b, c;Buchan et al, 1976). We now report a study of the metabolism of androgens by ovary-independent tumours derived by transplantation.…”

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Hormonal status and steroid metabolism in two transplantable rat mammary tumours

Miller¹,

Stewart²,

Hawkins³

1979

Br J Cancer

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HUMAN and rat mammary tumours metabolize steroid hormones and thus have the capacity to modify their local hormonal environment (Adams & Wong, 1968;Jones et al., 1970;King et al., 1964;Miller et al., 1974). In previous studies, we have investigated the metabolism of testosterone by a predominantly ovary-dependent DMBA-induced mammary tumour, and have shown it to be sensitive to levels of other hormones present in vitro (Miller, 1976a), or in vivo (Miller, 1976b, c;Buchan et al., 1976). We now report a study of the metabolism of androgens by ovary-independent tumours derived by transplantation. By using multiple tumours transplanted to a single rat, it has been possible to examine tumours derived from the same host animal before and after 2 endocrine manipulations.All animals were of an inbred strain of Sprague-Dawley rat, obtained from the Animal Diseases Research Association (ADRA), Moredun Institute, Edinburgh. Two lines of transplantable tumours were used: TG3 and TG5. These were histologically carcinomas, originally induced in female "ADRA" rats at 50 days of age by i.v. administration of 5 mg of DMBA. Both tumours were then serially transplanted by dorsal skin implantation of tumour fragments into neonatally-thymectomized hosts. At the time of study TG3 was at its 6th passage and TG5 was at its 7th passage.Three separate portions of a single donor tumour were implanted at different dorsal sites in each neonatally thymectomized host animal at 50 days of age. Tumours were measured twice weekly throughout the period of study. When the largest tumour reached 1 5 x 1 5 cm in size it was removed through a dorsal skin incision and the animal was immediately bilaterally oophorectomized. Ten days later, a further tumour was excised. The animals were then given daily injections of oestradiol-17f (1 jig in 0-2 ml corn oil) for a further 10 days.The remaining tumour was excised and the animals were killed, no injection of oestradiol being given on the day of death. Eight rats were so treated (4 with TG3 tumours and 4 with TG5 tumours). Control animals (2 with TG3 tumours and 2 with TG5 tumours) were treated similarly except that a sham oophorectomy was performed in place of an oophorectomy and the injection vehicle replaced oestradiol.After excision, portions of each tumour (0.5 g) were used for steroid-metabolism studies. Each was finally sliced at 0°C in Krebs-Ringer phosphate buffer pH 7-4 (5 ml). An NADPH-generating system and radioactive precursor(20(lCi of either 7a(3H) dehydroepiandrosterone (DHA) or 7a(3H) testosterone) was added. The incubation systems were shaken at 37°C in an atmosphere of 02 for 2 h (TG3 series) or 1 h (TG5 series on account of the high activity found in the tumour lines). The reaction was stopped by adding methanol to 80% v/v and the incubations stored at

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In vitro effects of oestrogen on 5α reduction of testosterone in hormone-dependent rat mammary carcinomata

Cited by 5 publications

References 11 publications

Hormonal status and testosterone metabolism of DMBA-induced rat mammary carcinomas

Hormonal status and testosterone metabolism of DMBA-induced rat mammary carcinomas

Effects of serial passage on the endocrine response and steroid metabolism of a rat mammary carcinoma

Hormonal status and steroid metabolism in two transplantable rat mammary tumours

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