In vitro effects of nanoparticles on renal cells

L’Azou, B; Jorly, Joana; On, Dinhill; Sellier, Elisabeth; Moisan, Frédéric; Fleury‐Feith, Jocelyne; Cambar, J.; Brochard, Patrick; Ohayon-Courtès, Céline

doi:10.1186/1743-8977-5-22

Cited by 126 publications

(86 citation statements)

References 46 publications

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“…3), using the MTT assay that measures the combined effects of cell proliferation and metabolic activity of cells. Our results were validated by directly counting the number of surviving cells using the trypan blue staining assay because the MTT assay is prone to artifacts under certain experimental conditions (L'Azou et al, 2008). The cell viability in cell culture medium containing non-coated TiO 2 was reduced to almost 50% of control values at the highest concentration examined (100 mg/L), which was in agreement with earlier studies of P25-TiO 2 (Liu et al, 2010;Park et al, 2008).…”

Section: Most Of the Toxicological Results On Nps Have Been Generatedsupporting

confidence: 76%

In vitro cytotoxicity and genotoxicity studies of titanium dioxide (TiO2) nanoparticles in Chinese hamster lung fibroblast cells

Hamzeh

Sunahara

2013

Toxicology in Vitro

115

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/npsi/ctrl?lang=en http://nparc.cisti-icist.nrc-cnrc.gc.ca/npsi/ctrl?lang=fr Access and use of this website and the material on it are subject to the Terms and Conditions set forth at http://nparc.cisti-icist.nrc-cnrc.gc.ca/npsi/jsp/nparc_cp.jsp?lang=en NRC Publications Archive Archives des publications du CNRCThis publication could be one of several versions: author's original, accepted manuscript or the publisher's version. / La version de cette publication peut être l'une des suivantes : la version prépublication de l'auteur, la version acceptée du manuscrit ou la version de l'éditeur. For the publisher's version, please access the DOI link below./ Pour consulter la version de l'éditeur, utilisez le lien DOI ci-dessous.http://dx.doi.org/10. 1016/j.tiv.2012.12.018 Toxicology in Vitro, 27, 2, pp. 864-873, 2012-12-28 In vitro cytotoxicity and genotoxicity studies of titanium dioxide (TiO2) nanoparticles in Chinese hamster lung fibroblast cells Hamzeh, Mahsa; Sunahara, Geoffrey I. Therefore, our objective was to investigate the cytotoxicity and genotoxicity of commercially available TiO 2 nanoparticles with respect to their selected physicochemical properties, as well as the role of surface coating of these nanoparticles. While all types of tested TiO 2 samples decrease cell viability in a mass-based concentration-and size-dependent manner, the polyacrylate-coated nano-TiO 2 product was only cytotoxic at higher concentrations. A similar pattern of response was observed for induction of apoptosis/necrosis, and no DNA damage was detected in the polyacrylate-coated nano-TiO 2 model. Given the increasing production of TiO 2 nanoparticles, toxicological studies should take into account the physiochemical properties of these nanoparticles that may help researchers to develop new nanoparticles with minimum toxicity.

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Section: Most Of the Toxicological Results On Nps Have Been Generatedsupporting

confidence: 76%

In vitro cytotoxicity and genotoxicity studies of titanium dioxide (TiO2) nanoparticles in Chinese hamster lung fibroblast cells

Hamzeh

Sunahara

2013

Toxicology in Vitro

115

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“…Nasal mucosa cells Cytotoxic, genotoxic and pro-inflammatory effects [77] Hepatocytes Inflammatory and genotoxic effects [56] Renal cells Cytotoxicity [78] Neurons Neurotoxic effect by disturbing the electrical activity of neuronal networks [7] Lung epithelial cells Cytotoxicity [79] Carbon black nanoparticles (CB-NPs) Lymphocytes Induction of chromosomal aberrations [80] Hepatocytes Hepatotoxicity [81] Neurons Dopaminergic neurons damage pathways [82] Lung epithelial cells Toxicity and inflammatory response. [83] Lymphocytes Cytotoxicity and genotoxicity [84] Silica nanoparticles (SiO 2 ) Fibroblast Cytotoxicity [25] Hepatocytes Genotoxicity, carcinogenicity, hepatotoxicity and inflammation [48,85] Renal cells Induction of oxidative stress and cytotoxicity [86] Neurons Neurotoxicity [7] Lung epithelial cells Genotoxicity, mutagenicity and carcinogenicity [87,88] Lymphocytes Induction of oxidative stress and reduction of immune capacity, Genotoxicity [27,80] Titanium dioxide nanoparticles (TiO 2 )…”

Section: Hepatocytesmentioning

confidence: 99%

Nanoparticle Technology as a Double-Edged Sword: Cytotoxic, Genotoxic and Epigenetic Effects on Living Cells

Jennifer¹,

Wnuk²

2013

JBNB

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Nanoparticles are considered as powerful tools in nanotechnological applications. Due to their unique physicochemical properties, their interactions with different biological systems have been shown. Nanomaterials have been successfully used as coating materials or treatment and diagnosis tools. Nevertheless, toxic effects of nanoparticles in vitro and in vivo have also been reported. Here, we summarize the current state of knowledge on exposure routes, cellular uptake and toxicological activities of the commonly used nanoparticles. In this context, we discuss the mechanisms of toxicity of nanoparticles involving perturbation of redox milieu homeostasis and cellular signaling pathways.

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“…The small size of nanomaterials provides them with specific properties, such as high surface-to-volume ratio and high surface charge (Linse et al, 2007). It has been reported that nanomaterials translocate from the interstitial region of the lung after respiratory exposure to other organs, such as the liver and kidney, through blood circulation (L'Azou et al, 2008;Nemmar et al, 2002;Semmler et al, 2004;Elder and Oberdörster, 2006).…”

Section: Introductionmentioning

confidence: 99%

Sub-toxic concentrations of nano-ZnO and nano-TiO<sub>2</sub> suppress neurite outgrowth in differentiated PC12 cells

et al. 2017

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-Nanomaterials have been extensively used in our daily life, and may also induce health effects and toxicity. Nanomaterials can translocate from the outside to internal organs, including the brain. For example, both nano-ZnO and nano-TiO 2 translocate into the brain via the olfactory pathway in rodents, possibly leading to toxic effects on the brain. Although the effects of nano-ZnO and nano-TiO 2 on neuronal viability or neuronal excitability have been studied, no work has focused on how these nanomaterials affect neuronal differentiation and development. In this study, we investigated the effects of nanoZnO and nano-TiO 2 on neurite outgrowth of PC12 cells, a useful model system for neuronal differentiation. Surprisingly, the number, length, and branching of differentiated PC12 neurites were significantly suppressed by the 7-day exposure to nano-ZnO (in the range of 1.0 × 10 -4 to 1.0 × 10 -1 μg/mL), at which the cell viability was not affected. The number and length were also significantly inhibited by the 7-day exposure to nano-TiO 2 (1.0 × 10 -3 to 1.0 μg/mL), which did not have cytotoxic effects. These results demonstrate that the neurite outgrowth in differentiated PC12 cells was suppressed by sub-cytotoxic concentrations of nano-ZnO or nano-TiO 2 .

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In vitro effects of nanoparticles on renal cells

Cited by 126 publications

References 46 publications

In vitro cytotoxicity and genotoxicity studies of titanium dioxide (TiO2) nanoparticles in Chinese hamster lung fibroblast cells

In vitro cytotoxicity and genotoxicity studies of titanium dioxide (TiO2) nanoparticles in Chinese hamster lung fibroblast cells

Nanoparticle Technology as a Double-Edged Sword: Cytotoxic, Genotoxic and Epigenetic Effects on Living Cells

Sub-toxic concentrations of nano-ZnO and nano-TiO<sub>2</sub> suppress neurite outgrowth in differentiated PC12 cells

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