In vitro effects of mammalian leptin, neuropeptide-Y, β-endorphin and galanin on transcript levels of thyrotropin β and common α subunit mRNAs in the pituitary of bighead carp (aristichthys nobilis)

Chowdhury, Indrajit; Chien, Jung-Tsun; Chatterjee, Abhijit; Yu, John Yuh-Lin

doi:10.1016/j.cbpc.2004.06.007

Cited by 31 publications

(15 citation statements)

References 62 publications

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“…On the contrary, NPY exhibits an inhibitory action on the TSHb expression in Japanese eel. Similar findings have been reported in mammals and in bighead carp [15,20,42], suggesting that the role of NPY on the hypothalamic-pituitary-thyroid (HPT) axis is conserved from fish to mammals. It has been proposed that the physiological significance of NPY in suppressing the HPT axis may be associated with maintaining energy homeostasis in organism under conditions of negative energy balance [42].…”

Section: Discussionsupporting

confidence: 79%

Structural and functional characterization of neuropeptide Y in a primitive teleost, the Japanese eel (Anguilla japonica)

Zhao

Xiao

et al. 2012

General and Comparative Endocrinology

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Section: Discussionsupporting

confidence: 79%

Structural and functional characterization of neuropeptide Y in a primitive teleost, the Japanese eel (Anguilla japonica)

Zhao

Xiao

et al. 2012

General and Comparative Endocrinology

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“…Cells were exposed to treatment for 6 h at which time cells were harvested and used for real-time PCR study. The time point was selected based on previously published data for bighead carp [13]. The experiment for each treatment group was carried out in triplicate wells.…”

Section: Bioactivity Assay Of Recombinant Gnpy: In Vitro Experimentsmentioning

confidence: 99%

“…Grouper pituitary was prepared for culture by the methods of Chen et al [12] and Chowdhury et al [13] with minor modifications. Briefly, the pituitary was excised after decapitation, washed three times with culture medium and cut manually into fragments (less than 1 mm 3 ).…”

Section: Bioactivity Assay Of Recombinant Gnpy: In Vitro Experimentsmentioning

confidence: 99%

“…In addition to its major roles on food intake and energy balance, in mammals, the NPY system has been reported to be involved in a broad range of physiological functions including hormonal release [3,36], obesity [27], stress response [40], anxiety [17] and sleep [19]. In fish, NPY is also involved in a variety of regulatory functions such as vasorelaxation [50], innate immunity [7], stress regulation [18] and pituitary hormone secretion [5,13,23].…”

Section: Introductionmentioning

confidence: 98%

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Stimulatory effects of neuropeptide Y on the growth of orange-spotted grouper (Epinephelus coioides)

Lin

et al. 2012

General and Comparative Endocrinology

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“…Because GnRH, NPY, and leptin were not homologously isolated from the Chinese soft-shell turtle and because turtle forms of these hormones were not commercially available, heterologous salmon GnRH, rat NPY, and rat leptin were used in this turtle system. From our previous observations (10,11), these heterologous hormones exhibit bioactivity that stimulates pituitary hormone expression in lower vertebrates, including the Chinese soft-shell turtles. DMEM, antibiotics, antimycotics, and the DNA marker were purchased from GIBCO BRL (New York, NY).…”

Section: Methodsmentioning

confidence: 99%

A preprogalanin cDNA from the turtle pituitary and regulation of its gene expression

Yu¹,

Pon²,

Ku³

et al. 2007

American Journal of Physiology-Regulatory, Integrative and Comp

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Galanin is a hormone 29 or 30 amino acids (aa) long that is widely distributed within the body and exerts numerous biological effects in vertebrates. To fully understand its physiological roles in reptiles, we analyzed preprogalanin cDNA structure and expression in the turtle pituitary. Using the Chinese soft-shell turtle (Pelodiscus sinensis order Testudines), we obtained a 672-base pair (bp) cDNA containing a 99-bp 5Ј-untranslated region, a 324-bp preprogalanin coding region, and a 249-bp 3Ј-untranslated region. The open-reading frame encoded a 108-aa preprogalanin protein with a putative 23-aa signal sequence at the NH 2 terminus. Based on the location of putative Lys-Arg dibasic cleavage sites and an amidation signal of Gly-Lys-Arg, we propose that turtle preprogalanin is processed to yield a 29-aa galanin peptide with Gly 1 and Thr 29 substitutions and a COOH-terminal amidation. Sequence comparison revealed that turtle preprogalanin and galanin-29 had 48 -81% and 76 -96% aa identities with those of other vertebrates, respectively, suggesting their conservative nature. Expression of the turtle galanin gene was detected in the pituitary, brain, hypothalamus, stomach, liver, pancreas, testes, ovaries, and intestines, but not in the adipose or muscle tissues, suggesting tissue-dependent differences. An in vitro study that used pituitary tissue culture indicated that treatment with 17␤-estradiol, testosterone, or gonadotropin-releasing hormone resulted in increased galanin mRNA expression with dose-or time-dependent differences, whereas leptin and neuropeptide Y reduced galanin mRNA levels. These results suggest a hormone-dependent effect on hypophyseal galanin mRNA expression.estrogen; androgen; gonadotropin-releasing hormone; neuropeptide Y; leptin GALANIN, a 29-amino acid (aa) hormone, was first isolated from porcine intestine and used to stimulate contractions of rat smooth muscle and to cause hyperglycemia in dogs (52). Since its discovery, galanin has also been found to exhibit numerous actions in vertebrates. For example, it induces feeding and the release of pituitary hormones [i.e., luteinizing hormone (LH) and growth hormone (GH)], decreases circulating levels of oxytocin, plays an antinociceptive role in the spinal cord, is involved in gastrointestinal motility and autonomic function, inhibits memory and learning, and promotes smooth muscle contractions (12). Moreover, clinical investigations have shown that galanin levels and galanin receptors are related to human disorders, such as Alzheimer disease and obesity (12). In reptiles, galanin can control gastrointestinal blood flow (24) and female oviposition (29). Although its mechanism of action in these reptilian physiological processes remains unknown, galanin in mammals reportedly operates via G i /G 0 -proteincoupled receptors involving effector systems such as K ϩ channels, Ca 2ϩ channels, and adenylate cyclase (3). In mice and humans, the galanin gene contains six exons and five introns (14, 26), and its mRNA consists of 398 -989 base pairs (bp),...

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In vitro effects of mammalian leptin, neuropeptide-Y, β-endorphin and galanin on transcript levels of thyrotropin β and common α subunit mRNAs in the pituitary of bighead carp (aristichthys nobilis)

Cited by 31 publications

References 62 publications

Structural and functional characterization of neuropeptide Y in a primitive teleost, the Japanese eel (Anguilla japonica)

Structural and functional characterization of neuropeptide Y in a primitive teleost, the Japanese eel (Anguilla japonica)

Stimulatory effects of neuropeptide Y on the growth of orange-spotted grouper (Epinephelus coioides)

A preprogalanin cDNA from the turtle pituitary and regulation of its gene expression

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