Structural and functional characterization of neuropeptide Y in a primitive teleost, the Japanese eel (Anguilla japonica)

Li, Shuisheng; Zhao, Lin; Xiao, Ling; Liu, Qiongyu; Zhou, Wei; Qi, Xin; Chen, Huapu; Yang, Huirong; Liu, Xiaochun; Zhang, Yong; Lin, Haoran

doi:10.1016/j.ygcen.2012.07.018

Cited by 10 publications

(6 citation statements)

References 57 publications

(81 reference statements)

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“…For example, in goldfish, NPY expression has been detected in the forebrain including the telencephalon, preoptic area, the olfactory bulb and thalamic regions [26]. In the Japanese eel Anguilla japonica , NPY is highly expressed in the telencephalon, optic tectum thalamus and hypothalamus [27]. NPY stimulates LH release in fish [28], has a role in growth hormone (GH) and gonadotropin hormone (GTH) secretion [29–31] and may coordinate growth, feeding and reproduction in fish [2].…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, the NPY response in goldfish depends on reproductive status [34]. Food deprivation in A. japonica also causes increases of NPY mRNA levels in the brain [27]. Changes in feeding behavior and regulation of energy expenditure are expected to accompany the maturation process in fish, particularly in species such as A. anguilla that allocate a lot of resources to reproduction [35].…”

Section: Discussionmentioning

confidence: 99%

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Changes in the gene expression profiles of the brains of male European eels (Anguilla anguilla) during sexual maturation

et al. 2014

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BackgroundThe vertebrate brain plays a critical role in the regulation of sexual maturation and reproduction by integrating environmental information with developmental and endocrine status. The European eel Anguilla anguilla is an important species in which to better understand the neuroendocrine factors that control reproduction because it is an endangered species, has a complex life cycle that includes two extreme long distance migrations with both freshwater and seawater stages and because it occupies a key position within the teleost phylogeny. At present, mature eels have never been caught in the wild and little is known about most aspects of reproduction in A. anguilla. The goal of this study was to identify genes that may be involved in sexual maturation in experimentally matured eels. For this, we used microarrays to compare the gene expression profiles of sexually mature to immature males.ResultsUsing a false discovery rate of 0.05, a total of 1,497 differentially expressed genes were identified. Of this set, 991 were expressed at higher levels in brains (forebrain and midbrain) of mature males while 506 were expressed at lower levels relative to brains of immature males. The set of up-regulated genes includes genes involved in neuroendocrine processes, cell-cell signaling, neurogenesis and development. Interestingly, while genes involved in immune system function were down-regulated in the brains of mature males, changes in the expression levels of several receptors and channels were observed suggesting that some rewiring is occurring in the brain at sexual maturity.ConclusionsThis study shows that the brains of eels undergo major changes at the molecular level at sexual maturity that may include re-organization at the cellular level. Here, we have defined a set of genes that help to understand the molecular mechanisms controlling reproduction in eels. Some of these genes have previously described functions while many others have roles that have yet to be characterized in a reproductive context. Since most of the genes examined here have orthologs in other vertebrates, the results of this study will contribute to the body of knowledge concerning reproduction in vertebrates as well as to an improved understanding of eel biology.Electronic supplementary materialThe online version of this article (doi:10.1186/1471-2164-15-799) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Changes in the gene expression profiles of the brains of male European eels (Anguilla anguilla) during sexual maturation

et al. 2014

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“…The signal peptide and neuropeptide prohormone cleavage sites in tac3s were predicted using SignalP 4.1 (31) and NeuroPred software (32, 33), respectively. Putative seven-transmembrane domains were predicted using the TMHMM server v. 2.0 (http://www.cbs.dtu.dk/services/TMHMM-2.0/) (34).…”

Section: Methodsmentioning

confidence: 99%

“…Putative seven-transmembrane domains were predicted using the TMHMM server v. 2.0 (http://www.cbs.dtu.dk/services/TMHMM-2.0/) (34). Multiple sequences were aligned and compared using Clustal X (35), and a phylogenetic tree was constructed by MEGA 6 using the neighbor-joining method (33, 36).…”

Section: Methodsmentioning

confidence: 99%

TAC3 Gene Products Regulate Brain and Digestive System Gene Expression in the Spotted Sea Bass (Lateolabrax maculatus)

Zhang

Wen

et al. 2019

Front. Endocrinol.

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Neurokinin B (NKB) is a member of the tachykinin ( tac ) family that plays important roles in mammalian growth by modulating prolactin (PRL) synthesis and secretion and causing contraction of the stomach and intestine. However, its potential role in regulating growth of teleosts is less clear. We aimed to explore the role that NKB plays in regulating fish growth using the spotted sea bass ( Lateolabrax maculatus ) as a model. In the present study, two tac3 and two tacr3 genes were identified in the spotted sea bass. Sequence analysis showed that two tac3 transcripts, tac3a and tac3b , encode four NKBs: NKBa-13, NKBa-10, NKBb-13, and NKBb-10. Expression analysis in different tissues showed that both genes are highly expressed in the brain, stomach and intestine of the spotted sea bass. In situ hybridization indicated that the tac3a and tac3b mRNAs are both localized in several brain regions, such as the telencephalon and hypothalamus, and that tacr3a and tacr3b are localized in the intestinal villus and gastric gland. To investigate the potential role of NKBs in regulating growth, in vitro experiments were performed to detect the effect of NKBs on growth-related gene expression in the brain and brain-gut peptide (BGP)-related genes in the stomach and intestine. NKBb-13 was the most critical ligand in regulating the expression of growth-related genes in the brain and brain-gut peptide (BGP)-related genes in the stomach. The expression of cholecystokinin ( cck ) was enhanced by NKBa-13, NKBa-10, and NKBb-10 but not NKBb-13 in the intestine. In general, our results showed that NKBs participate in regulating the growth of spotted sea bass.

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“…To date, the npy gene has been cloned in Carassius auratus (Blomqvist, Soderberg, Lundell, Milner, & Larhammar, ), Oncorhynchus mykiss (Doyon, Gilmour, Trudeau, & Moon, ), Epinephelus sp. (Chen, Li, & Lin, ), Oreochromis mossambicus (Carpio et al, ), Siniperca chuatsi (Liang, Li, Yao, Cheong, & Liao, ), Gadus morhua (Kehoe & Volkoff, ), Cetorhinus maximus (Larsson et al, ), Anguilla japonica (Li et al, ), and Paralichthys olivaceus (Wang et al, ), and the tissue distribution of its product has been studied. Results have shown that the mRNA expression of npy was detected in most tissues, and it was highly expressed in the brain.…”

Section: Introductionmentioning

confidence: 99%

Cloning, expression profiling, and effects of fasting status on neuropeptide Y in Schizothorax davidi

et al. 2019

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To better comprehend the mechanism that neuropeptide Y (npy) regulates feeding in Schizothorax davidi, we cloned and identified the full‐length cDNA sequence of the npy gene in this species using RACE technology. Subsequently, we explored the npy mRNA distribution in 18 tissues and investigated the expression of npy mRNA at postprandial and fasting stages. We found that the npy full‐length cDNA sequence is 803 bp. Moreover, npy mRNAs extensively expressed in all detected tissues, with the highest expression in hypothalamus. In postprandial study, the expression of npy mRNA in the hypothalamus was significantly decreased after eating (p < 0.01). In addition, the expression of the npy gene was significantly increased on the fifth day after fasting (p < 0.05). However, after refeeding, the expression of the npy gene was decreased significantly on days 9, 11, and 14 (p < 0.01). Our research suggest that npy may have an orexigenic role in S. davidi. Practical applications S. davidi, a coldwater fish native to China, has high economic value, and it has gained great popularity. To date, there is still no large‐scale breeding of S. davidi in China. How to strengthen the production performance of S. davidi is a hot research area. Neuropeptide Y (NPY), a 36‐amino‐acid single‐chain polypeptide, is one of the main appetite regulation factors. However, to date, no studies have reported on the biological function of npy in the feeding of S. davidi. In our study, we revealed that the trend of hypothalamic npy expression during the postprandial and fasting stages. The results suggested that npy might be an appetite‐promoting factor in this species. Overall, we provide the theoretical basis for how to strengthen the production performance of S. davidi through appetite regulation.

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Structural and functional characterization of neuropeptide Y in a primitive teleost, the Japanese eel (Anguilla japonica)

Cited by 10 publications

References 57 publications

Changes in the gene expression profiles of the brains of male European eels (Anguilla anguilla) during sexual maturation

Changes in the gene expression profiles of the brains of male European eels (Anguilla anguilla) during sexual maturation

TAC3 Gene Products Regulate Brain and Digestive System Gene Expression in the Spotted Sea Bass (Lateolabrax maculatus)

Cloning, expression profiling, and effects of fasting status on neuropeptide Y in Schizothorax davidi

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