In vitro effects of fluor-hydroxyapatite, fluorapatite and hydroxyapatite on colony formation, DNA damage and mutagenicity

Jantová, Soňa; Theiszová, Marica; Letašiová, Silvia; Bírošová, Lucia; Palou, T.M.

doi:10.1016/j.mrgentox.2008.01.010

Cited by 29 publications

(22 citation statements)

References 31 publications

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“…In addition, F 2 itself retains advantages over other ions in that it protects teeth from dental caries, especially in the environment of the oral cavity; it also enhances mineralisation and crystallisation. Some studies have revealed that FA has good biocompatibility, [24][25][26] and this gives a good opportunity to extend the research to FHAs. These materials can be used in different fields of surgery medicine as surface coating on various orthopaedic prostheses and dental implants.…”

Section: Introductionmentioning

confidence: 99%

RETRACTED: Effect of fluorine ion addition on structural, thermal, mechanical, solubility and biocompatibility characteristics of hydroxyapatite nanopowders

Eslami

Solati‐Hashjin

Tahriri

2010

Advances in Applied Ceramics

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In this study, the effect of fluorine ion addition on the structural, thermal, mechanical and solubility characteristics of hydroxyapatite nanopowders was investigated. The fluorine substituted hydroxyapatite powders with compositions of Ca 5 (PO 4 ) 3 (OH) 12x F x [x50 (hydroxyapatite), x50?68 (fluorhydroxyapatite) and x50?97 (fluorapatite)] were prepared. The powders were uniaxially pressed and were formed as disc shape. Subsequently, sinterability and thermal stability of disc samples were compared together. Also the effects simultaneously of fluoride content and temperature on the lattice parameters, crystallites size, microstrain and microstructure of the samples were examined. The sintering density and flexural strength of these samples were evaluated with the sintering temperature. Finally, the in vitro dissolution studies of the apatite samples were performed at osteoclastic resorption conditions. The above mentioned experimental results showed that the fluorine content had significant impact on the sintering behaviour, densification and mechanical properties of the hydroxyapatite nanopowders.

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Section: Introductionmentioning

confidence: 99%

RETRACTED: Effect of fluorine ion addition on structural, thermal, mechanical, solubility and biocompatibility characteristics of hydroxyapatite nanopowders

Eslami

Solati‐Hashjin

Tahriri

2010

Advances in Applied Ceramics

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“…The degree of the living cells can be measured through reading the results on a multi-well scanning spectrophotometer (ELISA reader) [18,19]. For this test, FA and 9CuFA concentrated elutes (not diluted) were prepared by adding culture medium supplemented with penicillin and streptomycin to FA and 9CuFA powder (1 mL to 100 mg of powder), according to the literature [20]. These elutes were then diluted by culture medium to the test concentrations (100%, 75%, 50%, 25%, 10%, 5%, and 1%) of FA and 9CuFA.…”

Section: Characterizationmentioning

confidence: 99%

“…For higher concentrations, cell viability decreases steadily until it reaches the lowest at 100%. The observed cytotoxicity of FA at high concentrations is due to the presence of uoride in the structure of FA [20]; however, it is worth mentioning that uoride has high biological activity and wide biomedical applications to stimulate bone formation. For 9CuFA, for all concentrations, the viability percentage is lower than the control sample, which reveals that the presence of Cu ions in the apatite structure results in an increase of the cytotoxicity e ect of uorapatite against BT-20 cell line.…”

Section: Fe-sem and Tem Observationsmentioning

confidence: 99%

Effect of Cu2+ ion on Biological Performance of Nanostructured Fluorapatite Doped with Copper

2017

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Abstract. Nanostructured copper-doped uorapatite (Cu x .Ca (10 x) .(PO 4 ) 6 .F 2 ) having crystallite sizes of 19, 29, and 34 nm at x = 0:9, 0.4, and 0.0, respectively, was synthesized by planetary ball milling of CaO, P2O5, CaF2, and CuO powders. Speci cations of the products were determined by Fourier-transform infrared spectroscopy, eld emission scanning electron microscopy, transmission electron microscopy, and X-ray di raction analyses. In-vitro studies and Mossman's Tetrazole Test (MTT) assays were also conducted by incubating Cu x .Ca (10 x) .(PO 4 ) 6 .F 2 powder into Kokubo's Simulated Body Fluid (SBF) and against BT-20 cell, respectively, to determine bioactivity and biocompatibility of the materials. Antibacterial e ects on Staphylococcus aureus were assessed by the disc di usion test method. Measurements showed that the rate of formation of uorapatite was lowered by Cu content. Besides, in-vitro experiments showed the same SBF interacted apatite precipitation for all samples. In contrast, MTT assays revealed di erent behavior for pure uorapatite and apatite with x = 0:9 Cu against BT-20 cell after 24 h of incubation. This highlights the increase of uorapatite cytotoxicity when Cu ion is present in the apatite structure. Copper-doped uorapatite was, however, desirably antibacterial. This stemmed from copper ions interactions with the bacterial metabolism which resulted in enzymes neutralization and copper-doped uorapatite antibacterial behavior.

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“…FA (Ca10(PO4)6F2, FA) can be synthetically prepared from hydroxyapatite and contains a suitable molecular concentration of OH-groups and F -ions [2]. When doped with lanthanides (Eu 3+ Tb 3+ or Gd 3+ ), this material exhibits special photoluminescent and has the potential for application in biomedical studies [3][4][5].…”

Section: Introductionmentioning

confidence: 99%

Synthesis of Europium-doped Fluorapatite Nanorods and Their Multifunctional Biomedical Applications

Zeng¹,

Sun²,

Wu³

et al. 2017

Preprint

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Europium (Eu)-doped fluorapatite (FA) nanorods have a biocompatibility similar to that of hydroxyapatite (HA) for use as cell imaging biomaterials due to their luminescent property. Here, we discuss the new application of europium-doped fluorapatite (Eu-FA) nanorods as an anticancer drug carrier. The Eu-FA nanorods were prepared by using a hydrothermal method. The morphology, crystal structure, fluorescence and composition were investigated. The specific crystal structure enables the effective loading of drug molecules. Doxorubicin (DOX), which was used as a model anticancer drug, effectively loaded onto the surface of the nanorods. The DOX release was pH dependent and occurred more rapidly at pH 5.5 than pH 7.4. The intracellular penetration of the DOX-loaded Eu-FA nanorods (Eu-FA/DOX) can be imaged in situ due to the self-fluorescence property. Treatment of melanoma A375 cells with Eu-FA/DOX elicited a more effective apoptosis rate than direct DOX treatment. Overall, Eu-FA exhibits potential for tracking and treating tumors and may be potentially useful as a multifunctional carrier system to effectively load and sustainably deliver drugs.

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In vitro effects of fluor-hydroxyapatite, fluorapatite and hydroxyapatite on colony formation, DNA damage and mutagenicity

Cited by 29 publications

References 31 publications

RETRACTED: Effect of fluorine ion addition on structural, thermal, mechanical, solubility and biocompatibility characteristics of hydroxyapatite nanopowders

RETRACTED: Effect of fluorine ion addition on structural, thermal, mechanical, solubility and biocompatibility characteristics of hydroxyapatite nanopowders

Effect of Cu2+ ion on Biological Performance of Nanostructured Fluorapatite Doped with Copper

Synthesis of Europium-doped Fluorapatite Nanorods and Their Multifunctional Biomedical Applications

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