In vitro effects of cadmium and DDVP (dichlorvos) on intestinal carbohydrase and protease activities in freshwater teleosts

Голованова, И. Л.; Кузьмина, В. В.; Gobzhelian, T.E; Pavlov, D. F.; Чуйко, Г. М.

doi:10.1016/s0742-8413(98)10063-4

Cited by 9 publications

(8 citation statements)

References 14 publications

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“…Inhibitions of amylase and cellulase in Daphnia magna after short-term exposure to Hg and Cd were reported by De Coen & Janssen (1997). In 11 freshwater teleost species exposed to 50 mg Cd l -1 , amylase activity was significantly lowered (Golovanova et al 1999). In the present study, clear intersite differences pointed out the adverse effect of environmental conditions on the activities of these enzymes.…”

supporting

confidence: 71%

Assessment of the health status of populations of the ragworm Nereis diversicolor using biomarkers at different levels of biological organisation

Kálmán¹,

Palais²,

Amiard³

et al. 2009

Mar. Ecol. Prog. Ser.

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We compared behavioural (burrowing speed), biochemical (acetylcholinesterase [AChE] inhibition, activities of digestive enzymes) and physiological (feeding and egestion rates, condition) responses in Nereis diversicolor originating from a multipolluted estuary (Loire estuary) and a relatively clean site (Bay of Bourgneuf) on 3 occasions during spring 2008. Significant inhibition of AChE and digestive enzymes (amylase and carboxymethylcellulase) activities were recorded in individuals from the Loire estuary compared to the reference site. At the individual level no significant intersite differences in burrowing speed were observed when ragworms were exposed to their sediment of origin. No link between AChE activity and burrowing behaviour was found in worms from either site. Cross-tests revealed that behavioural disturbances in locomotion were not due to neurological dysfunction but to avoidance of contaminated sediments. Feeding and egestion rates were depressed significantly in worms originating from the Loire estuary compared to the reference site. These behavioural impairments were accompanied by changes in digestive enzyme activities, which can explain the generally poorer condition of ragworms from the Loire estuary. Temporal changes in responses reported in the present study underline the importance of the fluctuation of environmental factors and thus the need for more than one sampling series to assess the health status of a given site. Disturbances at the different levels of biological organisation are likely explained by environmental contamination either chronic in the Loire estuary or the result of a small oil spill. KEY WORDS: Nereis diversicolor · Estuarine sediment · Biomonitoring · Behavioural impairments · Biochemical biomarkers Resale or republication not permitted without written consent of the publisherMar Ecol Prog Ser 393: [55][56][57][58][59][60][61][62][63][64][65][66][67] 2009 of sediment toxicity tests to examine the impact of pollutants on the aquatic sedimentary environment (DelValls & Conradi 2000, Chapman et al. 2002. In addition, field studies provide information about the biological effects of pollutants at various levels of biological organisation.Although biochemical and physiological biomarkers have been used increasingly in environment toxicology, they are not yet able to predict the effects of contaminants at higher levels of biological organisation. The aim of most ecological risk assessments is to protect populations, communities and ecosystems; only rarely is the goal to protect individuals. Therefore it is important to study the link between the responses of biomarkers at the sub-organismal and supraorganismal levels (Forbes et al. 2006). Studies of biomarkers related to energy metabolism, growth or reproduction (De Coen & Janssen 2003, Durou et al. 2007b as well as behavioural disturbances (Dell'Omo 2002, Amiard-Triquet 2009) appear particularly promising. Behavioural biomarkers are sensitive tools that help us to assess the impact of contaminants at concent...

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supporting

confidence: 71%

Assessment of the health status of populations of the ragworm Nereis diversicolor using biomarkers at different levels of biological organisation

Kálmán¹,

Palais²,

Amiard³

et al. 2009

Mar. Ecol. Prog. Ser.

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“…Ions of Cd (0.75 and 10 mg/l) in vitro caused a statistically significant (10 and 37%, respectively) decrease in common carp intestine α -amylase activity [28]. have confirmed that the effect depends on toxicant concentration and fish species specificity [64]. have confirmed that the effect depends on toxicant concentration and fish species specificity [64].…”

Section: Effects Of Heavy Metals On the Physiological And Biochemicalmentioning

confidence: 80%

“…As it was revealed, the carbohydrases of food organisms are more sensitive to toxic action of HM ions compared to the enzymes of fish digestive tract. In summer, when the functional activity of the digestive system is high, the carbohydrase sensitivities to HM increase [5,7,64]. As a rule, changes in pH or decrease in temperature lead to a 2-3-fold decrease of inhibiting effect.…”

Section: Effects Of Heavy Metals On the Physiological And Biochemicalmentioning

confidence: 99%

Effects of heavy metals on the physiological and biochemical status of fishes and aquatic invertebrates

Голованова

2008

Inland Water Biol

100

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“…Since a few hours after adding chlorophos to water this compound in fact entirely turns to DDVP [26], it is likely that main toxic effects are determined by the later in particular. It was shown that in vitro DDVP at concentrations of 0.2-100 mg/L does not affect the activities of digestive gly cosidases in eleven species of freshwater bony fishes [21]. However, chronic (60 days) exposure to DDVP at sublethal concentration of 0.46 mg/L triggers different changes in the amylolytic activity of intestinal mucosa in Mozambique tilapia (Oreochromis mossambicus Peters) [20].…”

Section: Discussionmentioning

confidence: 99%

The effect of organic toxicants on sensitivity of intestinal glycosidases to Cu and Zn in juvenile roach

Филиппов

Голованова

2012

Inland Water Biol

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The chronic effect (duration of exposure 218 days) of polychlorinated biphenyls (PCBs) and the prolonged effect of the short term action of chlorophos or of N methyl N' nitro N nitrosoguanidine (MNNG) during embryogenesis upon the sensitivity of intestinal glycosidases to Cu and Zn was studied in roach (Rutilus rutilus (L.) underyearlings. The Cu +2 and Zn +2 ions at concentrations ranging from 0.1 to 25 mg/L in vitro cause a 10-77% decrease in amylolytic activity in the intestinal mucosa of control roach. An elevated level of PCBs (50.8 ng/g wet weight of food and 426 ng/g dry weight of ground) increased the sen sitivity of glycosidases to Cu and Zn. The embryotoxic action of chlorophos at concentrations of 1 × 10 -6 -1 × 10 -2 mg/L in most cases increased the inhibitory effect of Cu but decreased that of Zn. As a rule, MNNG (3 × 10 -7 -3 × 10 -1 mg/L) reduced the glycosidase sensitivity to the effect of metal ions. The magnitude and direction of the effect depend on the nature and concentration of toxicants.

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In vitro effects of cadmium and DDVP (dichlorvos) on intestinal carbohydrase and protease activities in freshwater teleosts

Cited by 9 publications

References 14 publications

Assessment of the health status of populations of the ragworm Nereis diversicolor using biomarkers at different levels of biological organisation

Assessment of the health status of populations of the ragworm Nereis diversicolor using biomarkers at different levels of biological organisation

Effects of heavy metals on the physiological and biochemical status of fishes and aquatic invertebrates

The effect of organic toxicants on sensitivity of intestinal glycosidases to Cu and Zn in juvenile roach

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