The effect of organic toxicants on sensitivity of intestinal glycosidases to Cu and Zn in juvenile roach

Abstract

The chronic effect (duration of exposure 218 days) of polychlorinated biphenyls (PCBs) and the prolonged effect of the short term action of chlorophos or of N methyl N' nitro N nitrosoguanidine (MNNG) during embryogenesis upon the sensitivity of intestinal glycosidases to Cu and Zn was studied in roach (Rutilus rutilus (L.) underyearlings. The Cu +2 and Zn +2 ions at concentrations ranging from 0.1 to 25 mg/L in vitro cause a 10-77% decrease in amylolytic activity in the intestinal mucosa of control roach. An elevated level of PCBs (50.8 ng/g wet weight of food and 426 ng/g dry weight of ground) increased the sen sitivity of glycosidases to Cu and Zn. The embryotoxic action of chlorophos at concentrations of 1 × 10 -6 -1 × 10 -2 mg/L in most cases increased the inhibitory effect of Cu but decreased that of Zn. As a rule, MNNG (3 × 10 -7 -3 × 10 -1 mg/L) reduced the glycosidase sensitivity to the effect of metal ions. The magnitude and direction of the effect depend on the nature and concentration of toxicants.