In vitro effectiveness of Anidulafungin against Candida sp. biofilms

Abstract

This study furnishes deeper insights to previous works on anidulafungin, demonstrating the potent activity against Candida strains planktonic cells and biofilms. Candida sp., associated with many biomaterial-related infections, give rise to infective pathologies typically associated with biofilm formation. We recently determined the in vitro antifungal activities of echinocandin anidulafungin in association with some antifungal drugs against some Candida strains in their planktonic states. A total of 11 Candida strains biofilms were tested in this study: six Candida albicans, three C. parapsilosis and two C. tropicalis. All yeast isolates and ATCC strains were stored at -20°C in glycerol stocks and were subcultured on antimicrobial agent-free Sabouraud dextrose agar plates. MIC endpoints were determined colorimetrically by using the indicator 2,3-bis(2-methoxy-4-nitro-5-sulphophenyl)-5-[(phenylamino)carbonyl]-2H-tetrazolium hydroxide (XTT) with menadione as electron-coupling agent. The activity of anidulafungin was assessed using in vitro microbiological model relevant for clinical practice. Anidulafungin showed a strong activity in vitro against both planktonic and biofilms cells, and our study confirms that high anidulafungin concentrations might establish paradoxical growth effect in C. albicans and C. tropicalis biofilms.

The Role of Antifungals against Candida Biofilm in Catheter-Related Candidemia

“…Even so, caspofungin and micafungin were active against C. albicans and C. glabrata biofilms, but not against C. tropicalis and C. parapsilosis biofilms [ 65 , 66 ]. Anidulafungin has also been tested alone and in combination with other antifungals and proved highly active against both planktonic and sessile cells, with planktonic and sessile MICs for 50% and 90% of the isolates tested (MIC 50 and MIC 90 ) of ≤0.03 µg/mL to <0.125 µg/mL and ≤0.03 µg/mL to ≤0.03 µg/mL, respectively [ 67 , 68 ]. When anidulafungin was compared with AmB, it displayed more activity than AmB against C. albicans biofilms after 24 h of maturation; however, AmB was more active against more mature biofilms (>48 h) [ 69 ].…”

Elucidation of the synergistic action of Mentha Piperita essential oil with common antimicrobials

“…Bacterial resistance to antibiotic therapy is a growing emergency [ 1 ]. In the last years, several research programs have focused on designing new compounds possessing potential antimicrobial activity in order to avoid this problem [ 2 – 6 ]. New sources, especially plant-derived antimicrobial compounds, have been extensively studied in recent years [ 7 , 8 ].…”

Anidulafungin liposome nanoparticles exhibit antifungal activity against planktonic and biofilm Candida albicans

“…These concentrations correspond to an anidulafungin MIC range of 0.020–0.039, 0.049–0.098, and 0.025–0.049 μg/ml for 1×, 5×, and 10× liposomes, respectively. Non‐liposome‐encapsulated anidulafungin exhibits an MIC value of 0.01 μg/ml against C. albicans 10231 (Rosato et al, 2013).…”

“…In this study, we examined the anidulafungin liposome efficacy against both planktonic and biofilm C. albicans . Using a broth microdilution assay, the MIC values of all three liposome formulations examined against planktonic C. albicans when converted to equivalent anidulafungin concentration based on DL values for each formulation remained constant (0.039–0.098 μg/ml) and unchanged from free anidulafungin MIC values against C. albicans 10231 (Rosato et al, 2013). As expected, the corresponding liposome concentration required to inhibit Candida growth decreased with increasing DL.…”