IN VITRO EFFECT OF TERPENOIDS - RICH EXTRACT OF Momordica charantia ON ALPHA GLUCOSIDASE ACTIVITY

Abstract

Background: Momordica charantia is a vine, native to tropics and subtropics that has found use in herbal medicine. In Nigeria, the plant is locally used in the treatment of diabetes. Objective: To investigate the inhibitory effect of terpenoid-rich extract from Momordica charantia on α-glucosidase activity. Method: The inhibitory effect of the terpenoid-rich extract from Momordica charantia on the activity of alpha-glucosidase from Saccharomyces cerevisiae as well as the determination of the mode of inhibition were investigated using in vitro assay methods. Results: The results revealed that the terpenoid-rich extract had a high IC 50 of 1.60 mg/mL. The Lineweaver-Burk plot revealed that the extract exhibited partially mixed-type inhibition of α-glucosidase. Conclusions: It can be inferred from this study that although terpenoid-rich extract inhibited α-glucosidase, further purification will be necessary on the extract so as to increase its potency as an inhibitor of α-glucosidase. The action of the extract on α-glucosidase provided an insight into the probable mechanism of action of Momordica charantia in reducing hyperglycemia.

Inhibitory evaluation ofCurculigo latifoliaon α-glucosidase, DPP (IV) andin vitrostudies in antidiabetic with molecular docking relevance to type 2 diabetes mellitus

“…From the results, the inhibitory activity on both of the extracts shows explicated a remarkable inhibition on the a-glucosidase enzyme by revealing the percentage inhibition by root extracts is closer with the percentage of inhibition by marketed anti-diabetic drugs, acarbose. Previously, the findings of the current study are in a good agreement with other research works indicate that SWE extracts are capable of influencing a-glucosidase inhibitory capacity 21 .…”

Formulation of Whole Wheat Bitter Gourd-Supplemented Cookies for Improving Blood Glycemic Levels: Insights of Inhibition Assays and Antioxidant Activities

“…BG’s inhibitory ability is attributed to its rich phytochemical composition, including alkaloids, terpenes, flavonoids, phenols, curcubitanes, and lignans . These compounds, along with other complexes like momordicines, bind covalently to α-amylase enzyme (that is present in salivary and pancreatic secretions) and cause an alteration in its activity by forming compounds like quinones or lactones that counter nucleophilic groups on the α-amylase molecule Terpenoids present in BG are revealed to be the principal compound in inhibiting α-glucosidase enzyme . Furthermore, flavonols, notably quercetin and anthocyanin, played a significant role in α-amylase inhibition through competitive and noncompetitive inhibition .…”

“…49 These compounds, along with other complexes like momordicines, bind covalently to α-amylase enzyme (that is present in salivary and pancreatic secretions) and cause an alteration in its activity by forming compounds like quinones or lactones that counter nucleophilic groups on the α-amylase molecule 50 Terpenoids present in BG are revealed to be the principal compound in inhibiting α-glucosidase enzyme. 51 Furthermore, flavonols, notably quercetin and anthocyanin, played a significant role in α-amylase inhibition through competitive and noncompetitive inhibition. 52 Notably, the choice of extraction method, solvent, and harvesting period also significantly influence the α-amylase and α-glucosidase inhibition potential of BG.…”

Enzyme Inhibitory and Mutagenicity Guided Investigation of Selected Medicinal Plants in Different Solvents

“…Regarding M. charantia, the least enzyme inhibition activity was given by ethanolic extract (45.48 ± 0.48), while the trend in ascending order for other fractions was as follows: ethyl acetate < n-hexane < chloroform < n-butanol. According to Sallau et al [12], terpenoid-rich extract of bitter gourd had the highest IC50 value (1.60 mg/mL) against glucosidase enzyme. Earlier, Nhiem et al [13] also documented moderate antidiabetic efficacy in terms of inhibition of alpha glucosidase in crude extracts as well as purified constituents from M. charantia.…”