In vitro effect of human growth hormone on lymphocyte transformation and lymphocyte growth factors secretion

Abstract

Abstract. Cultures of human blood peripheral lymphocytes were performed in the presence or absence of human growth hormone, and also of phytohemagglutinin and normal human serum 10%. After incubation for 48 h, the supernatants were tested for their ability to promote the uptake of [3H]thymidine into lectin-activated lymphocytes. Supernatants from lymphocyte-free control samples, treated in the same manner, were assayed under the same experimental conditions. Variance analysis of the different dose-response relationships was performed. The results of these in vitro experiments confirm that physiological levels of GH inhibit the lectin-induced lymphoproliferation and that lymphocytes secrete an 'activity' able to stimulate the incorporation of [3H]thymidine into lectin activated lymphocytes. Furthermore we show that: 1) Secretion of this lymphocyte-stimulating activity is increased by physiological levels of GH; 2) This lymphocytic secretion is not radioimmunoassayable IGF-I; 3) Using fast protein liquid chromatography (FPLC), this activity appears in fractions with various molecular weights.

A review of bovine growth hormone

“…increased proliferation at low doses of GH (450 ng mL-') while higher test doses (up to 8500 ng ml-r) induced a progressive decrease in lymphocyte proliferation (Bozzola et al 1988a). In another study (Schimpff and Repellin 1989), physiological concentrations of GH added to the cultured cells inhibited mitogeninduced lymphocyte proliferation but the conditioned medium from these cells enhanced proliferation of subsequent cultures. Wolf et al (1987) For personal use only.…”

Production of Interleukin-1α and Interleukin-2 by Mononuclear Cells from Children with Growth Delay in Relation to the Degree of Growth Hormone Deficiency: Effects of Substitutive Treatment

“…These changes include defective anti body and cell-mediated immunity as assessed in vivo [24], reduced activity of natural killer cells [9,10] and reduced serum thymidine activity, i.e. activity able to stimulate the thymidine incorporation into lectin-acti vated lymphocytes [ 13,14], The role of GH and of GHdependent serum growth factors on the transformation of lymphocytes has already been demonstrated in pre vious works [13][14][15][16], We have reported in vitro [15] as well as in vivo [ 16] a GH-dependent production of mito genic factors by lymphocytes. Furthermore, the hypothe sis that GH could act on the production of ILs was sup ported by the report of Kelley et al [ 17], who showed that GH3 pituitary adenoma cells increase the synthesis of IL-2 in rats.…”

“…In previous works we reported that thymidine uptake into lectin-activated lymphocytes was stimulated in part by GH-dependent serum factors [13][14][15] and we demon strated a GH-dependent production of mitogenic factors with low molecular weights by lymphocytes [ 16]. So it is questionable whether the GH-dependent production of mitogenic factors by lymphocytes is related to their pro duction of interleukins (ILs).…”

Effect of Recombinant Growth Hormone and Chromium Picolinate on Cytokine Production and Growth Performance in Swine

“…As an immunomodulator, growth hormone increases antibody synthesis in rodents [ 1 ], mitogen-induced prolif eration of human, mouse, rat, and bovine lymphocytes [1,13,14], in vitro IL-2 synthesis [1], macrophage and neu trophil oxidative burst [15][16][17], antibacterial activity [ 16], and in vivo hematopoiesis [18,19]. Cattle treated with growth hormone demonstrated reduced production of TNF-a following in vivo administration of bacterialderived endotoxin [20].…”