In vitro effect of carboplatin, cytarabine, paclitaxel, vincristine, and low‐power laser irradiation on murine mesenchymal stem cells

Horvat-Karajz, K.; Balogh, Zsuzsanna; Kovács, Viktória; drRerNat, András Hámori; Sréter, L; Uher, Ferenc

doi:10.1002/lsm.20791

Cited by 49 publications

(9 citation statements)

References 40 publications

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“…De Villiers et al [21] Human adipose tissue 24, 48, and 72 h after irradiation The irradiated cell group had a statistically significant increase on cell proliferation at 24 and 72 h when compared to the nonirradiated group Horvat-Karajz et al [22] Murine bone marrow 24, 48, and 72 h after the first irradiation Biostimulation at lower doses in all time point evaluated and inhibition in cell growth after 48 h at higher doses (11.7 J/cm 2 ) Mvula et al [23] Human adipose tissue 24 and 48 h after irradiation Increase of cell proliferation in irradiated cells when compared to nonirradiated cells at the intervals of 24 and 48 h Ong et al [24] Human orbital fat tissues 24, 48, and 72 h after irradiation LED irradiation showed no effect on human orbital fat stem cells proliferation Soares et al [25] Human periodontal ligament 0, 24, 48, and 72 h after irradiation The dose of 1.0 J/cm 2 promoted a significant increase in cell proliferation at 48 and 72 h when compared to the control and the group that received a dose of 0.5 J/cm 2 Giannelli et al [26] Murine bone marrow 0, 24, 48, and 72 h after irradiation Cell proliferation was significantly higher at 72 h after irradiation compared to the control group Pereira et al [27] Healthy and inflamed human dental pulp 24, 48, 72, and 96 h after irradiation…”

Section: Discussionmentioning

confidence: 98%

Effect of low-level laser therapy on mesenchymal stem cell proliferation: a systematic review

et al. 2015

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Low-level laser therapy (LLLT) has been used in several in vitro experiments in order to stimulate cell proliferation. Cells such as fibroblasts, keratinocytes, lymphocytes, and osteoblasts have shown increased proliferation when submitted to laser irradiation, although little is known about the effects of LLLT on stem cells. This study aims to assess, through a systematic literature review, the effects of LLLT on the in vitro proliferation of mesenchymal stem cells. Using six different terms, we conducted an electronic search in PubMed/Medline database for articles published in the last twelve years. From 463 references obtained, only 19 papers met the search criteria and were included in this review. The analysis of the papers showed a concentration of experiments using LLLT on stem cells derived from bone marrow, dental pulp, periodontal ligament, and adipose tissue. Several protocols were used to irradiate the cells, with variations on wavelength, power density, radiation time, and state of light polarization. Most studies demonstrated an increase in the proliferation rate of the irradiated cells. It can be concluded that the laser therapy positively influences the in vitro proliferation of stem cells studied, being necessary to carry out further experiments on other cell types and to uniform the methodological designs.

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Section: Discussionmentioning

confidence: 98%

Effect of low-level laser therapy on mesenchymal stem cell proliferation: a systematic review

et al. 2015

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“…At the cell-layer level, the power density measured using a power meter (Ophir Optronics, Jerusalem, Israel) was 6.67 mW/cm 2 . Because biostimulation after irradiation may continue for 48 h [22], the cultures were irradiated every other day since in half of them the medium had been changed to ODM. The first irradiation day was set as day 0.…”

Section: Irradiation Proceduresmentioning

confidence: 99%

The effect of noncoherent red light irradiation on proliferation and osteogenic differentiation of bone marrow mesenchymal stem cells

Peng

Zheng³

et al. 2011

Lasers Med Sci

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Mesenchymal stem cells (MSCs) are promising for use in regenerative medicine. Low-level light irradiation (LLLI) has been shown to modulate various processes in different biological systems. The aim of our study was to investigate the effect of red light emitted from a light-emitting diode (LED) on bone marrow MSCs with or without osteogenic supplements. MSCs both with and without osteogenic supplements were divided into four groups, and each group was irradiated at doses of 0, 1, 2 and 4 J/cm(2). Cellular proliferation was evaluated using WST-8 and 5-ethynyl-2'-deoxyuridine (EdU) fluorescence staining. The alkaline phosphatase activity, mineralization, and expression of osteoblast master genes (Col1α1, Alpl, Bglap and Runx2) were monitored as indicators of MSC differentiation towards osteoblasts. In groups without osteogenic supplements, red light at all doses significantly stimulated cellular proliferation, whereas the osteogenic phenotype of the MSCs was not enhanced. In groups with osteogenic supplements, red light increased alkaline phosphatase activity and mineralized nodule formation, and stimulated the expression of Bglap and Runx2, but decreased cellular proliferation. In conclusion, nonconherent red light can promote proliferation but cannot induce osteogenic differentiation of MSCs in normal media, while it enhances osteogenic differentiation and decreases proliferation of MSCs in media with osteogenic supplements.

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“…The conditioning regimen is known to be able to damage not only the HSC but also the bone marrow (BM) microenvironment [5], [6]. It has been suggested that stromal damage caused by conditioning regimens or previous chemo-therapy treatments may have a profound influence on engraftment kinetics [10].…”

Section: Discussionmentioning

confidence: 99%

“…The conditioning regimen is toxic for both hematopoietic stem cells (HSC) and BM microenvironment [5], [6]. The latter is crucial to ensure normal hematopoietic engraftment after transplantation [7]–[9].…”

Section: Introductionmentioning

confidence: 99%

Bone Marrow Mesenchymal Stem Cells for Improving Hematopoietic Function: An In Vitro and In Vivo Model. Part 2: Effect on Bone Marrow Microenvironment

et al. 2011

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The aim of the present study was to determine how mesenchymal stem cells (MSC) could improve bone marrow (BM) stroma function after damage, both in vitro and in vivo. Human MSC from 20 healthy donors were isolated and expanded. Mobilized selected CD34+ progenitor cells were obtained from 20 HSCT donors. For in vitro study, long-term bone marrow cultures (LTBMC) were performed using a etoposide damaged stromal model to test MSC effect in stromal confluence, capability of MSC to lodge in stromal layer as well as some molecules (SDF1, osteopontin,) involved in hematopoietic niche maintenance were analyzed. For the in vivo model, 64 NOD/SCID recipients were transplanted with CD34+ cells administered either by intravenous (IV) or intrabone (IB) route, with or without BM derived MSC. MSC lodgement within the BM niche was assessed by FISH analysis and the expression of SDF1 and osteopontin by immunohistochemistry. In vivo study showed that when the stromal damage was severe, TP-MSC could lodge in the etoposide-treated BM stroma, as shown by FISH analysis. Osteopontin and SDF1 were differently expressed in damaged stroma and their expression restored after TP-MSC addition. Human in vivo MSC lodgement was observed within BM niche by FISH, but MSC only were detected and not in the contralateral femurs. Human MSC were located around blood vessels in the subendoestal region of femurs and expressed SDF1 and osteopontin. In summary, our data show that MSC can restore BM stromal function and also engraft when a higher stromal damage was done. Interestingly, MSC were detected locally where they were administered but not in the contralateral femur.

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In vitro effect of carboplatin, cytarabine, paclitaxel, vincristine, and low‐power laser irradiation on murine mesenchymal stem cells

Cited by 49 publications

References 40 publications

Effect of low-level laser therapy on mesenchymal stem cell proliferation: a systematic review

Effect of low-level laser therapy on mesenchymal stem cell proliferation: a systematic review

The effect of noncoherent red light irradiation on proliferation and osteogenic differentiation of bone marrow mesenchymal stem cells

Bone Marrow Mesenchymal Stem Cells for Improving Hematopoietic Function: An In Vitro and In Vivo Model. Part 2: Effect on Bone Marrow Microenvironment

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