The effect of noncoherent red light irradiation on proliferation and osteogenic differentiation of bone marrow mesenchymal stem cells

Peng, Fei; Wu, Hua; Zheng, Yangfan; Xu, Xianghuai; Yu, Jun

doi:10.1007/s10103-011-1005-z

Cited by 69 publications

(52 citation statements)

References 37 publications

(51 reference statements)

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“…In recent years, researchers have demonstrated the applicability of light-emitting diodes (LEDs) in experimental and clinical studies, promoting cell biostimulation similar to laser irradiation [12][13][14]. For LEDs emitting visible red and near infrared light energy, different primary targets and photoreactions can be found in target cells.…”

Section: Introductionmentioning

confidence: 99%

Effect of low-level laser therapy on mesenchymal stem cell proliferation: a systematic review

et al. 2015

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Low-level laser therapy (LLLT) has been used in several in vitro experiments in order to stimulate cell proliferation. Cells such as fibroblasts, keratinocytes, lymphocytes, and osteoblasts have shown increased proliferation when submitted to laser irradiation, although little is known about the effects of LLLT on stem cells. This study aims to assess, through a systematic literature review, the effects of LLLT on the in vitro proliferation of mesenchymal stem cells. Using six different terms, we conducted an electronic search in PubMed/Medline database for articles published in the last twelve years. From 463 references obtained, only 19 papers met the search criteria and were included in this review. The analysis of the papers showed a concentration of experiments using LLLT on stem cells derived from bone marrow, dental pulp, periodontal ligament, and adipose tissue. Several protocols were used to irradiate the cells, with variations on wavelength, power density, radiation time, and state of light polarization. Most studies demonstrated an increase in the proliferation rate of the irradiated cells. It can be concluded that the laser therapy positively influences the in vitro proliferation of stem cells studied, being necessary to carry out further experiments on other cell types and to uniform the methodological designs.

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Section: Introductionmentioning

confidence: 99%

Effect of low-level laser therapy on mesenchymal stem cell proliferation: a systematic review

et al. 2015

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“…SVF can be easily collected and enriched with platelet-rich plasma . In addition, ADMSCs in SVF can be activated by photostimulation with low-level lasers and light-emitting diodes [Peng et al, 2012]. Similar to drug studies, there is an effective cell dose (ECD) for stem cell therapy, which is defined as the minimum cell number required to discern a remarkable therapeutic effect [Horwitz et al, 2002].…”

Section: Systemic Injection Approachmentioning

confidence: 99%

Role of Mesenchymal Stem Cells in Bone Regenerative Medicine: What Is the Evidence?

Oryan

Kamali²,

Moshiri³

et al. 2017

Cells Tissues Organs

285

204

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Healing and regeneration of bone injuries, particularly those that are associated with large bone defects, are a complicated process. There is growing interest in the application of osteoinductive and osteogenic growth factors and mesenchymal stem cells (MSCs) in order to significantly improve bone repair and regeneration. MSCs are multipotent stromal stem cells that can be harvested from many different sources and differentiated into a variety of cell types, such as preosteogenic chondroblasts and osteoblasts. The effectiveness of MSC therapy is dependent on several factors, including the differentiating state of the MSCs at the time of application, the method of their delivery, the concentration of MSCs per injection, the vehicle used, and the nature and extent of injury, for example. Tissue engineering and regenerative medicine, together with genetic engineering and gene therapy, are advanced options that may have the potential to improve the outcome of cell therapy. Although several in vitro and in vivo investigations have suggested the potential roles of MSCs in bone repair and regeneration, the mechanism of MSC therapy in bone repair has not been fully elucidated, the efficacy of MSC therapy has not been strongly proven in clinical trials, and several controversies exist, making it difficult to draw conclusions from the results. In this review, we update the recent advances in the mechanisms of MSC action and the delivery approaches in bone regenerative medicine. We will also review the most recent clinical trials to find out how MSCs may be beneficial for treating bone defects.

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“…Although several studies have investigated the effects of LEDs with various wavelengths, such as 620 nm [14], 630 nm [43], and 660 nm [44] red light and 830 nm [15] near infrared light, on BMSCs, the available colors represent a broad range of wavelengths, which may produce different effects. Thus, it is necessary to further elucidate the effects of LEDs with different wavelengths and to determine the molecular mechanisms of these effects.…”

Section: Cellular Physiology and Biochemistry Cellular Physiology Andmentioning

confidence: 99%

“…Non-coherent red LED light has been shown to promote BMSC proliferation, but it has failed to induce osteogenic differentiation of BMSCs in normal medium. However, it can enhance osteogenic differentiation and decrease proliferation of BMSCs in osteogenic differentiation medium [14]. In addition, treatments with low-power LED irradiation in the red spectrum (620-660 nm wavelengths) and in the near infrared red (NIR) spectrum (830 nm wavelength) have been reported to promote BMSC proliferation [15].…”

Section: Introductionmentioning

confidence: 99%

Effects of Blue Light Emitting Diode Irradiation On the Proliferation, Apoptosis and Differentiation of Bone Marrow-Derived Mesenchymal Stem Cells

Yuan

Yan

Gong

et al. 2017

Cell Physiol Biochem

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Background/Aims: Blue light emitting diodes (LEDs) have been proven to affect the growth of several types of cells. The effects of blue LEDs have not been tested on bone marrowderived mesenchymal stem cells (BMSCs), which are important for cell-based therapy in various medical fields. Therefore, the aim of this study was to determine the effects of blue LED on the proliferation, apoptosis and osteogenic differentiation of BMSCs. Methods: BMSCs were irradiated with a blue LED light at 470 nm for 1 min, 5 min, 10 min, 30 min and 60 min or not irradiated. Cell proliferation was measured by performing cell counting and EdU staining assays. Cell apoptosis was detected by TUNEL staining. Osteogenic differentiation was evaluated by ALP and ARS staining. DCFH-DA staining and γ-H2A.X immunostaining were used to measure intracellular levels of ROS production and DNA damage. Results: Both cell counting and EdU staining assays showed that cell proliferation of BMSCs was significantly reduced upon blue LED irradiation. Furthermore, treatment of BMSCs with LED irradiation was followed by a remarkable increase in apoptosis, indicating that blue LED light induced toxic effects on BMSCs. Likewise, BMSC osteogenic differentiation was inhibited after exposure to blue LED irradiation. Further, blue LED irradiation was followed by the accumulation of ROS

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The effect of noncoherent red light irradiation on proliferation and osteogenic differentiation of bone marrow mesenchymal stem cells

Cited by 69 publications

References 37 publications

Effect of low-level laser therapy on mesenchymal stem cell proliferation: a systematic review

Effect of low-level laser therapy on mesenchymal stem cell proliferation: a systematic review

Role of Mesenchymal Stem Cells in Bone Regenerative Medicine: What Is the Evidence?

Effects of Blue Light Emitting Diode Irradiation On the Proliferation, Apoptosis and Differentiation of Bone Marrow-Derived Mesenchymal Stem Cells

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