In vitro duodenal lipolysis of lipid-based drug delivery systems studied by HPLC–UV and HPLC–MS

Wulff-Pérez, Miguel; Barrajón‐Catalán, Enrique; Micol, Vicente; Martín-Rodríguez, Agustín; Vicente, J. De; Gálvez-Ruiz, Marı́a José

doi:10.1016/j.ijpharm.2014.02.027

Cited by 12 publications

(7 citation statements)

References 31 publications

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“…There are a number of methods available in the literature to assess lipolysis in vitro such as titrometry, spectroscopy, chromatography, radioactivity, interfacial tensiometry, turbidimetry, conductimetry, immunochemistry, microscopy, and, more recently, light scattering and HPLC . However, as stated by Beisson et al, there is to date no single universal method of lipase assay, and the choice will depend on the user’s own specific requirements . Among current alternatives, pH stat titrator is to date the most used and appears very suitable in many cases. − Still, it has certain limitations as it can be used only within a restricted pH range and measure activities >0.1 (μmol FFA/min) …”

Section: Introductionmentioning

confidence: 99%

“…However, as stated by Beisson et al, there is to date no single universal method of lipase assay, and the choice will depend on the user’s own specific requirements . Among current alternatives, pH stat titrator is to date the most used and appears very suitable in many cases. − Still, it has certain limitations as it can be used only within a restricted pH range and measure activities >0.1 (μmol FFA/min) …”

Section: Introductionmentioning

confidence: 99%

“…Because lipolysis is an interfacial process, techniques based on surface tension offer an interesting platform to develop new methods to assess it. However, these have been to date much less used for various reasons . Back in 1990, Piéroni and co-workers developed a method based on monolayers by using a Langmuir film balance to study lipase inhibition by proteins .…”

Section: Introductionmentioning

confidence: 99%

“…There are a number of methods available in the literature to assess lipolysis in vitro such as titrometry, spectroscopy, chromatography, radioactivity, interfacial tensiometry, turbidimetry, conductimetry, immunochemistry, microscopy, and, more recently, light scattering and HPLC 21 . 22 However, as stated by Beisson et al, there is to date no single universal method of lipase assay, and the choice will depend on the user's own specific requirements. 22 Among current alternatives, pH stat titrator is to date the most used and appears very suitable in many cases.…”

Section: ■ Introductionmentioning

confidence: 99%

“…23−25 Still, it has certain limitations as it can be used only within a restricted pH range and measure activities >0.1 (μmol FFA/min). 22 Because lipolysis is an interfacial process, techniques based on surface tension offer an interesting platform to develop new methods to assess it. However, these have been to date much less used for various reasons.…”

Section: ■ Introductionmentioning

confidence: 99%

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Natural Inhibitors of Lipase: Examining Lipolysis in a Single Droplet

Castillo-Santaella

Maldonado-Valderrama

Cabrerizo-Vílchez

et al. 2015

J. Agric. Food Chem.

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Inhibition of lipase activity is one of the approaches to reduced fat intake with nutritional prevention promoting healthier diet. The food industry is very interested in the use of natural extracts, hence reducing the side effects of commercial drugs inhibiting lipolysis. In this work we propose a novel methodology to rapidly assess lipolysis/inhibition in a single droplet by interfacial tension and dilatational elasticity. The evolution of the interfacial tension of lipase in simplified duodenal fluid in the absence and that in the presence of the pharmaceutical drug Xenical are the negative (5 ± 1 mN/m) and positive (9 ± 1 mN/m) controls of the inhibition of lipolysis, respectively. Then, we correlate the inhibition with the reduction of the interfacial activity of lipase and further identify the mode of action of the inhibition based on dilatational response (conformational changes induced in the molecule/blocking of adsorption sites). This work provides new insight into the lipase inhibition mechanism and a rapid methodology to identify the potential of new natural inhibitors.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…There are a number of methods available in the literature to assess lipolysis in vitro such as titrometry, spectroscopy, chromatography, radioactivity, interfacial tensiometry, turbidimetry, conductimetry, immunochemistry, microscopy, and, more recently, light scattering and HPLC 21 . 22 However, as stated by Beisson et al, there is to date no single universal method of lipase assay, and the choice will depend on the user's own specific requirements. 22 Among current alternatives, pH stat titrator is to date the most used and appears very suitable in many cases.…”

Section: ■ Introductionmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

See 3 more Smart Citations