Natural Inhibitors of Lipase: Examining Lipolysis in a Single Droplet

Abstract

Inhibition of lipase activity is one of the approaches to reduced fat intake with nutritional prevention promoting healthier diet. The food industry is very interested in the use of natural extracts, hence reducing the side effects of commercial drugs inhibiting lipolysis. In this work we propose a novel methodology to rapidly assess lipolysis/inhibition in a single droplet by interfacial tension and dilatational elasticity. The evolution of the interfacial tension of lipase in simplified duodenal fluid in the absence and that in the presence of the pharmaceutical drug Xenical are the negative (5 ± 1 mN/m) and positive (9 ± 1 mN/m) controls of the inhibition of lipolysis, respectively. Then, we correlate the inhibition with the reduction of the interfacial activity of lipase and further identify the mode of action of the inhibition based on dilatational response (conformational changes induced in the molecule/blocking of adsorption sites). This work provides new insight into the lipase inhibition mechanism and a rapid methodology to identify the potential of new natural inhibitors.

Anti-Lipase Potential of the Organic and Aqueous Extracts of Ten Traditional Edible and Medicinal Plants in Palestine; a Comparison Study with Orlistat

“…In addition to this, Palestinian territory is covered with a huge number of plants and from this huge biodiversity, there are large numbers of them considered traditional medicines or foodssinceancient times [ 25 ]. From these plants, A. palaestinum , C. azarolus , M. parviflora , T. syriacum , R. coriaria , R. officinalis , P. guajava , O. dayi , B. nigra , and V. vinifera were investigated for their efficacy as natural anti-obesity products and compared with Orlistat, which considered the anti-lipase drug of choice [ 26 ].…”

Current understanding of antiobesity property of capsaicin

“…It can induce weight loss through several mechanisms such as (1) suppressing appetite and inducing satiety. [678] (2) enhancing lipid metabolism,[910] (3) enhancing thermogenesis,[1112] (4) inhibiting pancreatic lipase,[13141516] (5) inhibiting α-amylase activity,[171819] and (6) preventing adipocyte differentiation. [2021] In this review, we addressed the most recent studies about the antiobesity property of capsaicin and its possible mechanisms of action is also discussed.…”

Real-time monitoring of the hydrolysis of vegetable oils loaded in silica colloidal crystal films with lipase by optical interferometry

“…There are many in vitro studies evaluating the hydrolysis of lipids by lipases, such as by studying changes in the interfacial tension of individual droplets to assess the hydrolysis of lipids and constructing lipase membrane reactors to perform enzymatic catalysis at the macroscopic oil-water interface. 19,20 In addition, studies on free lipase using pH-Stat, 21 lipase hydrolysis in reverse micelles, 22 and the hydrolysis of lipids by immobilized lipase 23 have also been performed.…”