In vitro dopaminergic neuroprotective and in vivo antiparkinsonian-like effects of Δ3,2-hydroxybakuchiol isolated from Psoralea corylifolia (L.)

Abstract

Cocktail recipes containing Psoralea corylifolia seeds (PCS) are used to empirically treat Parkinson disease. A PCS isolate Delta(3),2-hydroxybakuchiol (BU) can inhibit dopamine uptake in dopamine transporter (DAT) transfected Chinese hamster ovary (CHO) cells, and dopamine reuptake blockade may provide an alternative approach for ameliorating parkinsonism. Here, we assessed the potential dopaminergic neuroprotective, and antiparkinsonian-like activity of BU. BU sample size was increased by using a scale-up extraction paradigm. Pharmacologically, BU significantly protected SK-N-SH cells from 1-methyl-4-phenylpyridinium (MPP(+)) insult, produced striking inhibitory actions on dopamine/norepinephrine uptake and WIN35,428 binding in synaptosomes on in vivo administration, and significantly preventing poor performance on rotarod and dopaminergic loss in substantia nigra in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) mice. BU acts by protecting dopaminergic neurons from MPP(+) injury and preventing against MPTP-induced behavioral and histological lesions in the Parkinson's disease (PD) model, possibly by inhibiting monoamine transporters. These findings suggest that BU could be meaningful in PD treatment.

Protective Effect of Psoralea corylifolia L. Seed Extract against Palmitate‐Induced Neuronal Apoptosis in PC12 Cells

“…Previously, we found that PCE has protective effects against high fat diet-induced inflammation in the liver and H 2 O 2 -induced apoptosis in beta cells [24, 31], suggesting that PCE might have ameliorative effects on diabetic complications. Neuroprotective effects of PCE against damage caused by neurotoxic agents, such as 3-nitropropionic acid and 1-methyl-4-phenylpyridinium [22, 23], have been previously reported, but the effect on neuronal cell death caused by glucotoxicity or lipotoxicity has not been studied. In this study, we investigated the protective effect of PCE against lipotoxicity, which is physiologically relevant, using neuron-like PC12 cells.…”

“…PCE contains a number of chemical compounds, such as coumarins (including psoralidin, psoralen, and isopsoralen) and meroterpenes (including bakuchiol and 3,2-hydroxybakuchiol). PCE or its single compounds show neuroprotective effects against cytotoxic insults such those caused by as 1-methyl-4-phenylpyridinium or 3-nitropropionic acid [22, 23], but its possible neuroprotective effects against glucotoxicity or lipotoxicity have not been studied. Therefore, we investigated the protective effect of PCE against palmitate- (PA-) induced lipotoxicity in rat pheochromocytoma PC12 cells and investigated the mechanisms involved in the antilipotoxic effect of PCE.…”

Isobavachalcone attenuates lipopolysaccharide-induced ICAM-1 expression in brain endothelial cells through blockade of toll-like receptor 4 signaling pathways

“…Recent studies have demonstrated that active fractions of the PCL seeds possess antimicrobial (Yin et al, 2004), immunomodulatory (Latha et al, 2000), antioxidative (Guo et al, 2005), antitumor (Sun et al, 1998), bone-strengthening (Lim et al, 2009;Tsai et al, 2007) and estrogenic activities (Lim et al, 2011). There are also reports suggesting that PCL extracts or constituents may be effective in the prevention and treatment of dementia (Hsieh et al, 2000), Parkinson's disease (Zhao et al, 2009) and ischemic stroke (Kim, 2005). PCL extracts contain a number of bioactive compounds such as flavonoids, coumarines and meroterpenes, which provide the pharmacological basis of its bioactivities (Zhao et al, 2005).…”

The Benzopyrone Biochanin-A as a reversible, competitive, and selective monoamine oxidase B inhibitor

“…The PCS seeds have been recently shown to have chemoprotective and antioxidant properties [19]. Some of its investigated phytochemicals showed phytoestrogenic [20], anti-inflammatory [21], and neuroprotective [22] properties. Moreover, the seed’s aqueous extract was found to inhibit DA and NE transporters [23].…”