Isobavachalcone attenuates lipopolysaccharide-induced ICAM-1 expression in brain endothelial cells through blockade of toll-like receptor 4 signaling pathways

Lee, Kwang Min; Kim, Jae Mi; Baik, Eun Joo; Ryu, Jin-Sook; Lee, Soo Hwan

doi:10.1016/j.ejphar.2015.02.013

Cited by 31 publications

(20 citation statements)

References 49 publications

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“…LPS stimulates IL-6, MCP-1 and VCAM-1 mRNA expression in endothelial cells [33]. Isobavachalcone significantly retards leukocyte adhesion to brain endothelial cell via the inhibition of TLR4 signaling [34]. Our results showed that LPS obviously enhanced both protein and mRNA levels of TLR4, while gene silencing of TLR4 inhibited the production of inflammatory mediators and adhesion molecules in LPS-treated cells.…”

Section: Discussionmentioning

confidence: 78%

Hypaphorine Attenuates Lipopolysaccharide-Induced Endothelial Inflammation via Regulation of TLR4 and PPAR-γ Dependent on PI3K/Akt/mTOR Signal Pathway

Sun

Zhu

Cai

et al. 2017

IJMS

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Endothelial lesion response to injurious stimuli is a necessary step for initiating inflammatory cascades in blood vessels. Hypaphorine (Hy) from different marine sources is shown to exhibit anti-inflammatory properties. However, the potential roles and possible molecular mechanisms of Hy in endothelial inflammation have yet to be fully clarified. We showed that Hy significantly inhibited the positive effects of lipopolysaccharide (LPS) on pro-inflammatory cytokines expressions, including tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), monocyte chemoattractant protein 1 (MCP-1) and vascular cellular adhesion molecule-1 (VCAM-1), as well as induction of the phosphorylation of Akt and mTOR in HMEC-1 cells. The downregulated peroxisome proliferator-activated receptor γ (PPAR-γ) and upregulated toll-like receptor 4 (TLR4) expressions in LPS-challenged endothelial cells were prevented by Hy. Inhibition of both PI3K and mTOR reversed LPS-stimulated increases in TLR4 expressions and decreases in PPAR-γ levels. Genetic silencing of TLR4 or PPAR-γ agonist pioglitazone obviously abrogated the levels of pro-inflammatory cytokines in LPS-treated HMEC-1 cells. These results suggest that Hy may exert anti-inflammatory actions through the regulation of TLR4 and PPAR-γ dependent on PI3K/Akt/mTOR signal pathways. Hy may be considered as a therapeutic agent that can potentially relieve or ameliorate endothelial inflammation-associated diseases.

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Section: Discussionmentioning

confidence: 78%

Hypaphorine Attenuates Lipopolysaccharide-Induced Endothelial Inflammation via Regulation of TLR4 and PPAR-γ Dependent on PI3K/Akt/mTOR Signal Pathway

Sun

Zhu

Cai

et al. 2017

IJMS

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“…Isobavachalcone, (E)‐1‐(2,4‐dihydroxy‐3‐[3‐methyl‐2‐butenyl]‐phenyl)‐3‐(4‐hydroxyphenyl)‐2‐propen‐1‐one or (E)‐4,2′,4′‐trihydroxy‐3′‐prenylchalcone; 2′,4,4′‐trihydroxy‐3′‐prenyl‐transchalcone (IBC), is a chalcone compound that was first isolated from Psoralea corylifolia in 1968 (Kuete & Sandjo, ). To date, IBC has been reported to exhibit diverse bioactivities, including antibacterial, antifungal, anticancer, neuroprotective, antitubercular, and antioxidant effects (Jing et al, ; Lee, Kim, Baik, Ryu, & Lee, ; Nishimura et al, ; Shi, Wu, Huo, Zhou, & Jin, ), and current research is focused on various natural substances containing IBC, which is abundant in some edible plants (Haraguchi, Inoue, Tamura, & Mizutani, ; Kim, Lim, Lee, & Jeong, ; Ohno et al, ). However, no studies have examined the roles of IBC and IBC‐containing natural substances in the recovery of damaged muscle cells.…”

Section: Introductionmentioning

confidence: 99%

Isobavachalcone attenuates myotube atrophy induced by TNF‐α through muscle atrophy F‐box signaling and the nuclear factor erythroid 2‐related factor 2 cascade

Hur

Kim

Choi

et al. 2018

Phytotherapy Research

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Skeletal muscle atrophy is a condition characterized by damaged muscle fibers and reduced numbers of muscle cells due to various causes. Muscle atrophy is associated with chronic diseases, such as heart failure, diabetes, and aging-related diseases.Isobavachalcone (IBC) is a flavonoid found in various foods and natural products, and studies have investigated its diverse effects, including its neuroprotective and anticancer effects. However, no studies have evaluated the effects of IBC on muscle atrophy. Thus, in this study, we assessed the effects of IBC on prevention of muscle atrophy. To evaluate the preventive effects of IBC on muscle atrophy, we used C2C12 myoblasts and induced muscle atrophy by tumor necrosis factor (TNF)-α. IBC regulated the expression levels of muscle atrophy F-box and muscle RINGfinger-1 in response to damaged muscle cells, thereby restoring the expression of myosin heavy chain and myogenin. Moreover, IBC regulated the phosphorylation of the nuclear factor-κB and p38 and upregulated the expression of nuclear factor erythroid 2-related factor 2 and heme oxygenase-1, which are involved in regulating oxidative stress. Our results indicated that IBC acted to relieve TNF-α-induced skeletal muscle atrophy by regulating the factors related to inflammation and oxidative stress.

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“…The components identified were corylinin (new compound), psoralen, neobavaisoflavone, sophoracoumestan A, uracil, and daidzin (Ruan et al, ). In 2015, two more new flavonoids, bakuisoflavone, and bakuflavanone were isolated from the fruit of P. corlylifolia with antimicrobial activities (Lee, Kim, Baik, Ryu, & Lee, ). In one study, six new flavonoid compounds and a meroterpenoid were isolated and identified by spectroscopic methods from P. corylifolia fruits and displayed medium activity against Staph.…”

Section: Phytochemical Constituentsmentioning

confidence: 99%

“…In 2015, two more new flavonoids, bakuisoflavone, and bakuflavanone were isolated from the fruit of P. corylifolia and were tested against the strains of MRSA (MRSA481 and MRSA 584) and it was found that two compounds showed some good antibacterial effects with MIC values of (>32 (>9.4) and >32 (>9.4)) and (>32 (>9.5) and >32 (>9.5 μg/ml)), respectively (Lee et al, ). Tissue culture studies showed that Jasmonic acid treated plants of P. corylifolia enhanced psoralen content in leaves and roots of P. corylifolia .…”

Section: Biological Activitiesmentioning

confidence: 99%

“…In another study, it was revealed that IBC, a flavonoid from P. corylifolia, has the ability to ameliorate the neuronal injury in brain diseases related to inflammation, and this was accomplished through inhibition of lipopolysaccharide induced intercellular adhesion molecule‐1 expression and leukocyte adhesion to brain endothelial cell by blocking toll‐like receptor 4 signaling (Lee et al, ).…”

Section: Biological Activitiesmentioning

confidence: 99%

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Psoralea corylifolia L: Ethnobotanical, biological, and chemical aspects: A review

Alam

Khan

Asad

2017

Phytotherapy Research

120

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Psoralea corylifolia L. (Leguminosae) is a well-known traditional medicinal plant used from ancient times for treatment of various ailments. It is widely distributed and an important part of therapeutics in Ayurveda and in Chinese medicines. The aim of this review is to present comprehensive and most up to date report on its ethnobotanical, ethnopharmacological, clinical, phytochemical, and side effects. Studies on the ethnobotanical, ethnopharmacological, clinical, phytochemical, and side effects of P. corylifolia were published until year 2017 and were searched using various scientific databases. The scientific literature searched revealed that these plant species has been extensively investigated in vivo and in vitro for various biological and phytochemical studies. It has cardiotonic, vasodilator, pigmentor, antitumor, antibacterial, cytotoxic, and anti-helminthic properties and locally used for alopecia, inflammation, leukoderma, leprosy, psoriasis, and eczema. So far, about a hundred bioactive compounds have been isolated from seeds and fruits, and most important compounds identified belongs to coumarins, flavonoids, and meroterpenes groups. This review article summarized the most updated scientific literature on bioactive phytochemical and biological activities of P. corylifolia. This article will be a useful addition to providing information for future research, and more standard clinical trials are needed for the plant to be used as therapeutic agent.

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Isobavachalcone attenuates lipopolysaccharide-induced ICAM-1 expression in brain endothelial cells through blockade of toll-like receptor 4 signaling pathways

Cited by 31 publications

References 49 publications

Hypaphorine Attenuates Lipopolysaccharide-Induced Endothelial Inflammation via Regulation of TLR4 and PPAR-γ Dependent on PI3K/Akt/mTOR Signal Pathway

Hypaphorine Attenuates Lipopolysaccharide-Induced Endothelial Inflammation via Regulation of TLR4 and PPAR-γ Dependent on PI3K/Akt/mTOR Signal Pathway

Isobavachalcone attenuates myotube atrophy induced by TNF‐α through muscle atrophy F‐box signaling and the nuclear factor erythroid 2‐related factor 2 cascade

Psoralea corylifolia L: Ethnobotanical, biological, and chemical aspects: A review

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