In vitro dissociation and reassociation of the symbionts of the lichenHeppia echinulata

Marton, Kela; Galun, Margalith

doi:10.1007/bf01623964

Cited by 27 publications

(17 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Various types of haustoria and vesicles have been reported in a number of lichens with unicellular and heterocystous cyanobionts (Paran, Ben-Shaul & Galun, 1971 ;Peveling, 1973;Marton & Galun, 1976;Bergman & HussDanell, 1983;Budel & Rhiel, 1987;Rai, 1990fe;Honegger, 1991). The type and structure of haustoria and the vesicles observed in L. confinis were similar to those in L. pygmaea (Peveling, 1973).…”

Section: Discussionmentioning

confidence: 76%

The marine lichen Lichina confinis (O. F. Müll.) C. Ag.: ultrastructure and localization of nitrogenase, glutamine synthetase, phycoerythrin and ribulose 1, 5‐bisphosphate carboxylase/oxygenase in the cyanobiont

1993

View full text Add to dashboard Cite

SUMMARYThallus ultrastructure and the localization of proteins involved in photosynthesis, N^ fixation and animonia assimilation was studied in the Lichina confinis (O. F. Mull) C. Ag.-Calothrix symbiosis. The lichen thallus showed profuse branching. Within this, the cyanobiont was located in the cortical zone. Haustoria were frequently observed in contact with vegetative cells of the cyanobiont but not with heterocysts. An extensive mucilage layer containing numerous vesicles occurred around cyanobiont cells. Immunolabelling showed nitrogenase to be exclusively located in heterocysts of the cyanobiont and the free-living Calothrix sp. Glutamine synthetase (GS) levels in the cyanobiont were found to be drastically decreased compared to the levels in free-living (cultured) Calothrix sp. Such a decrease was more prominent in mature parts of the thallus than in the apical region. Rubisco was mostly located in carboxysomes of the vegetative cells of the cyanobiont. There were relatively low levels of Rubisco in tbe cytoplasm of vegetative cells, wbile heterocysts sbowed negligible levels. In free-living (cultured) Calothrix sp., pbycoerytbrin (PE) was found to be located along tbe tbylakoid membranes both in vegetative cells and beterocysts. Cyanobiont cells sbowed a similar pattern but tbe levels were relatively lower.

show abstract

Section: Discussionmentioning

confidence: 76%

The marine lichen Lichina confinis (O. F. Müll.) C. Ag.: ultrastructure and localization of nitrogenase, glutamine synthetase, phycoerythrin and ribulose 1, 5‐bisphosphate carboxylase/oxygenase in the cyanobiont

1993

View full text Add to dashboard Cite

show abstract

“…In lichens, the role of the surface has not yet been examined in any detail, and any role it may play in the resynthesis of compatible symbionts is still unknown. The limited evidence available from resynthesis studies suggests that lichen mycobionts can discriminate between compatible and incompatible phycobionts (Ahmadjian and Henrikson, 1959;Ahmadjian, 1960Ahmadjian, , 1973Ahmadjian and Heikkila, 1970;Marton and Galun, 1976). Several other studies suggest that this may be due to cell surface recognition factors (Ahmadjian, Jacobs and Russell, 1978;Lockhart, Rowell and Stewart, 1978;Bubrick and Galun, 1980;Bubrick, Galun and FrensdorfT, 1981).…”

Section: Introductionmentioning

confidence: 99%

PHYTOLECTINS FROM THE NITROGEN‐FIXING LICHEN PELTIGERA HORIZONTALIS: THE BINDING PATTERN OF PRIMARY PROTEIN EXTRACT

Petit

1982

New Phytologist

View full text Add to dashboard Cite

SUMMARYA protein fraction containing phytolectins was isolated from the nitrogen-fixing lichen Peltigera horizontalis. These lectins were thermostable, truly non-specific and were more effective on human erythrocytes A, B and O than concanavalin A. Phytolectins originate from the mycobiont and do not bind any of the freshly isolated phycobionts tested, but with slight differences bind the cultured phycobionts isolated from three Peltigera spp. It is suggested that the production of phytolectins may be characteristic of the lichen symbiosis and that they may be involved in the recognition or initial interactions between compatible lichen symbionts.

show abstract

“…Cell characteristics of the co-inoculated symbionts in species of Xanthoria and Cladonia have been described for five stages of the interaction: a "pre-contact" stage shows symbionts which are not in physical contact but are close enough to share extracellular excretions; a "contact" stage shows the alga and fungus beginning to make physical contact by appressorial-like structures; a "growth together" stage shows the coordinated growth of the symbionts forming a cellular mass; a "transitional pre-thallus" stage shows the formation D r a f t of an undifferentiated thallus-like structure; and finally the production of a stratified thallus (Ahmadjian et al 1978;Galun 1988;Armaleo 1991). The last two stages often require additional culture conditions and have been less commonly investigated, but Marton and Galun (1976) provided evidence for the complete thallus formation in Heppia echinulata Marton and Galun. Other studies have also been successful in achieving complete thallus development (e.g., Thomas…”

Section: Isolation and Culturing Of Symbiontsmentioning

confidence: 99%

Interface between fungi and green algae in lichen associations

Piercey‐Normore

Athukorala

2017

Botany

View full text Add to dashboard Cite

It is widely recognized that the lichen is the product of a fungus and a photosynthetic partner (green alga or cyanobacterium) but its acceptance was slow to develop throughout history. The development of powerful microscopic and other lab techniques enabled better understanding of the interface between symbionts beginning with the contentious concept of the dual nature of the lichen thallus. Even with accelerating progress in understanding the interface between symbionts, much more work is needed to reach a level of knowledge consistent with that of other fungal interactions. This review describes the interface between algal and fungal symbionts in lichens, including the appearance of interacting symbionts, our current understanding of communication between symbionts, and emerging new concepts in light of the challenges and debates that have provided lichenology with an enriched history. Communication between symbionts occurs before physical contact, which has been studied by microscopy, movement of molecules between symbionts, and gene expression studies. New discoveries include the interaction with more than one alga in a lichen thallus as well as other unrelated fungi and bacteria typically found on or within the thallus. This mini review briefly describes our current state of knowledge and highlights new directions for further study.

show abstract

In vitro dissociation and reassociation of the symbionts of the lichenHeppia echinulata

Cited by 27 publications

References 4 publications

The marine lichen Lichina confinis (O. F. Müll.) C. Ag.: ultrastructure and localization of nitrogenase, glutamine synthetase, phycoerythrin and ribulose 1, 5‐bisphosphate carboxylase/oxygenase in the cyanobiont

The marine lichen Lichina confinis (O. F. Müll.) C. Ag.: ultrastructure and localization of nitrogenase, glutamine synthetase, phycoerythrin and ribulose 1, 5‐bisphosphate carboxylase/oxygenase in the cyanobiont

PHYTOLECTINS FROM THE NITROGEN‐FIXING LICHEN PELTIGERA HORIZONTALIS: THE BINDING PATTERN OF PRIMARY PROTEIN EXTRACT

Interface between fungi and green algae in lichen associations

Contact Info

Product

Resources

About