In vitro digestibility as screening tool for improved forage quality in triticale

Zutter, Anneleen De; Boever, Johan De; Muylle, Hilde; Roldán‐Ruiz, Isabel; Haesaert, Geert

doi:10.1016/j.fcr.2023.109009

Cited by 2 publications

(5 citation statements)

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“…This study builds further on the work presented in De Zutter et al [ 44 ], in which the variation in IVD and related subtraits was investigated, using a collection of winter triticale, harvested at the soft dough stage. Phenotypic data obtained from two consecutive growing seasons revealed moderate to high broad-sense heritability of the studied traits (H²: 0.50–0.95) and wide phenotypic variation for IVOMD, IVNDFD, and IVD related subtraits in triticale (coefficient of variation (CV): 3.9–16%).…”

Section: Introductionmentioning

confidence: 66%

“…It is known that the detection of associated SNP markers is more likely when large differences in phenotype are available [ 40 ]. Considerable variation and moderate to high H² for the investigated traits is shown in De Zutter et al [ 44 ]. Despite the relatively small population size (118 genotypes) compared to previous GWAS in triticale [ 41 – 43 , 73 ], 53 QTLs were identified over all investigated traits (Table 3 ).…”

Section: Discussionmentioning

confidence: 93%

“…4 ). A strong positive correlation (ρ = 0.79) was already observed between plant and stem IVNDFD at phenotypic level [ 44 ].The homozygous REF genotypic class of marker QTL 4211801_19_C/T displays higher plant and stem IVNDFD, suggesting that important loci controlling IVD in triticale are located on chromosome 1A (Fig. 4 ).…”

Section: Discussionmentioning

confidence: 94%

“…The studied triticale collection consisted of 118 winter triticale genotypes and included 101 commercial varieties and 17 breeding lines from European and North American breeding origin. A description of this collection is provided in Supplementary Table 1 and in De Zutter et al [ 44 ]. The European varieties represent seven important triticale breeding origins (Switzerland, Germany, Denmark, France, the Netherlands, Poland, and Romania).…”

Section: Methodsmentioning

confidence: 99%

“…All genotypes were harvested as forage at the soft dough maturity stage (GS85, Zadoks scale) [ 46 ]. In this study, only the subtraits previously found to be highly correlated with plant IVOMD, plant IVNDFD, and stem IVNDFD (Spearman correlation coefficient ρ > 0.50) [ 44 ] are considered, as listed in Supplementary Table 2 . These included stem length, ear proportion, total plant STA, total plant ADFom, and stem KL.…”

Section: Methodsmentioning

confidence: 99%

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Genome-wide association study for in vitro digestibility and related traits in triticale forage

De Zutter,

Piro,

Maenhout

et al. 2024

BMC Plant Biol

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Background Triticale is making its way on dairy farms as an alternative forage crop. This requires the availability of high-yielding triticale varieties with good digestibility. Triticale forage breeding mainly focussed on biomass yield, but efforts to improve digestibility are increasing. We previously investigated the interrelationships among different quality traits in soft dough triticale: starch, acid detergent fibre and in vitro digestibility of organic matter (IVOMD) and of neutral detergent fibre (IVNDFD) of the total plant, IVNDFD and Klason lignin of the stems, and ear proportion and stem length. Here we determine the genetic control of these traits, using a genome-wide association (GWAS) approach. A total of 33,231 DArTseq SNP markers assessed in a collection of 118 winter triticale genotypes, including 101 varieties and 17 breeding lines, were used. Results The GWAS identified a total of 53 significant marker-trait associations (MTAs). The highest number of significantly associated SNP markers (n = 10) was identified for total plant IVNDFD. A SNP marker on chromosome 1A (4211801_19_C/T; 474,437,796 bp) was found to be significantly associated with ear proportion, and plant and stem IVNDFD, with the largest phenotypic variation for ear proportion (R²p = 0.23). Based on MTAs, candidate genes were identified which were of particular relevance for variation in in vitro digestibility (IVD) because they are putatively involved in plasma membrane transport, cytoskeleton organisation, carbohydrate metabolic processes, protein phosphorylation, and sterol and cell wall biogenesis. Interestingly, a xyloglucan-related candidate gene on chromosome 2R, SECCE2Rv1G0126340, was located in close proximity of a SNP significantly associated with stem IVNDFD. Furthermore, quantitative trait loci previously reported in wheat co-localized with significantly associated SNP markers in triticale. Conclusions A collection of 118 winter triticale genotypes combined with DArTseq SNP markers served as a source for identifying 53 MTAs and several candidate genes for forage IVD and related traits through a GWAS approach. Taken together, the results of this study demonstrate that the genetic diversity available in this collection can be further exploited for research and breeding purposes to improve the IVD of triticale forage.

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Section: Introductionmentioning

confidence: 66%

Section: Discussionmentioning

confidence: 93%

Section: Discussionmentioning

confidence: 94%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Genome-wide association study for in vitro digestibility and related traits in triticale forage

De Zutter,

Piro,

Maenhout

et al. 2024

BMC Plant Biol

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Forage yields and nutritive values of oat and triticale pastures for grazing sheep in early spring

Hanoğlu Oral

2024

PeerJ

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Background Small-grain winter cereals can be utilized as early spring pastures in temperate climates to relieve grazing pressure and potentially mitigate feed shortages. This study was conducted to determine the effects of triticale and oat cereal pastures grazed by sheep during early spring on forage yields, nutritive values, and nutritional requirements of sheep. Methods The research was carried out over three consecutive years, from 2015 to 2017, at the Sheep Research Institute in Bandırma-Balıkesir, located in the Marmara region of Türkiye. The treatments were arranged in a completely randomized block design, with the two forage species, triticale and oat, randomized within each of three blocks. The animal material for the study consisted of 24 Karacabey Merino sheep, each 2 years old, with an average live weight of 57.6 ± 0.5 kg, all in the late lactation stage. In each replication, four sheep were included, resulting in a total of 12 sheep grazing in each of the triticale and oat pastures. The sheep grazed exclusively on the cereal pastures without any additional feed, and had unrestricted access to water throughout the entire period of the experiment. The dry matter yields (DMY), dry matter intakes (DMI), nutritive values, and mineral contents of the cereal species were determined. Results The DMY showed significant differences over the years (P < 0.05). No differences in DMY were observed between pastures, with oats yielding 11.99 t ha−1 and triticale yielding 11.08 t ha−1. During the grazing period, the change in DMY was significant in all years (P < 0.05). The average DMI of the sheep was 2,003.5 g d−1 for triticale and 2,156.6 g d−1 for oat, respectively, and DMI exhibited no significant differences across pastures. Although there was no difference in DMI between 2015 and 2016, the lowest consumption occurred in 2017 (P < 0.05). Additionally, while DMI showed different trends each year based on the periods, it generally decreased by the end of the grazing period. While both pastures provided similar nutritive values, significant differences were observed in the crude protein (CP), acid detergent fiber (ADF), in vitro true DM digestibility (DDM), and metabolisable energy (ME) values across the years. Over the years, as the grazing period progressed, CP levels decreased while neutral detergent fiber (NDF), ADF, and acid detergent lignin (ADL) increased, resulting in reduced DDM and ME values. The phosphorus (P) content in triticale was higher than in oats, but there were no differences in the content of other minerals between them. Between the years, significant differences were observed in the levels of phosphorus (P) and iron (Fe), while changes in other elements were insignificant. The variation in mineral content during the grazing process differed over the three years. Study results indicated that the nutritional values of triticale and oat pastures are similar, and both can effectively be used to provide sufficient feed to meet the early spring forage requirements for sheep.

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In vitro digestibility as screening tool for improved forage quality in triticale

Cited by 2 publications

References 41 publications

Genome-wide association study for in vitro digestibility and related traits in triticale forage

Genome-wide association study for in vitro digestibility and related traits in triticale forage

Forage yields and nutritive values of oat and triticale pastures for grazing sheep in early spring

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