In Vitro Digestibility and Fermentation of Mannooligosaccharides from Coffee Mannan

Abstract

Digestibility of mannooligosaccharides obtained from thermal hydrolysis of spent coffee grounds was examined by in vitro digestion method. Mannooligosaccharides were resistant to human salivary ␣-amylase, artificial gastric juice, porcine pancreatic enzymes and rat intestinal mucous enzymes. Fermentation products of mannooligosaccharides in human large intestine were estimated by in vitro fecal incubation method. Mannooligosaccharides were fermented by human fecal bacteria and the products of fermentation were short chain fatty acids. Acetic, propionic and n-butyric acids were the main short chain fatty acids as end fermentation products. These results suggest that mannooligosaccharides are indigestible saccharides and are converted to short chain fatty acids in human large intestine. The short chain fatty acids are thought to improve the large intestinal environment. Moreover, they are absorbed and utilized by the host as an energy source.

Effects of Mannooligosaccharides from Coffee on Microbiota and Short Chain Fatty Acids in Rat Cecum

“…A similar enlargement of the cecum was observed in rats fed a xylooligosaccharide diet (Campbell et al, 1997) or a galactooligosaccharide diet (Tsuchihashi et al, 1988;Djouzi & Andrieux, 1997). MOS are also indigestible oligosaccharides (Asano et al, 2003). It is assumed that the cecum of rats consuming a MOS diet enlarges: the cecum weight of rats fed a MOS diet tended to enlarge compared with those fed a control diet.…”

“…Oligosaccharides Mannooligosaccharides were prepared as described in a previous paper (Asano et al, 2003). Spent coffee grounds were hydrolyzed in a reactor that was kept at 220'C.…”

“…Furthermore, MOS were indigestible saccharides and fermented to short chain fatty acids by human fecal bacteria (Asano et al , 2003). The purpose of this study was to determine the in vivo effects of MOS on microbiota and short chain fatty acid production.…”

Effects of Mannooligosaccharides from Coffee Mannan on Fecal Microflora and Defecation in Healthy Volunteers

“…Furthermore, MOS were resistant to digestive enzymes and were fermented by human fecal bacteria. The fermentation products were short chain fatty acids (SCFA) (Asano et al, 2003). These results suggested that MOS were indigestible saccharides, which were selectively utilized by beneficial bacteria and converted to SCFA in the human large intestine.…”

“…Preparation of mannooligosaccharides (Asano et al, 2003) The thermally hydrolyzed product from spent coffee grounds was decolorized using active carbon powder (Umehachi; Taihei Chemicals, Osaka) and desalted using cation exchange resin (SKIB; Mitsubishi Chemicals) and anion exchange resin (WA30; Mitsubishi Chemicals, Tokyo). One hundred grams of a mixture of monosaccharides and MOS mixture were obtained from 300 g of the hydrolyzed products of spent coffee grounds.…”