In Vitro Differentiation of Zygotic Lines of Date Palm: Biochemical and Molecular Approaches to Sex Determination

Bekheet, S. A.; Solliman, Mohei El-Din; Taha, Hussein; Zaid, A.; Hegarty, Vincent; Kâabi, H.

doi:10.17660/actahortic.2007.736.10

Cited by 4 publications

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“…A similar result was reported by Bekheet et al [16] in their study on biochemical and molecular approaches to sex determination in date palm.…”

Section: Resultssupporting

confidence: 89%

Molecular Characterization of some Date palms in Nigeria Using RAPD Markers

2015

J App Biol Biotech

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Date palm (Phoenix dactylifera L.) is a long-lived, dioecious, monocotyledonous fruit tree having (2n=2x=36) chromosome number and wind pollinated member of the Arecaceae family. Identification of genetic variation and phylogenetic relationships in the date palm grown in Nigeria would be useful for genetic improvement and conservation. Genetic polymorphism in 10 date palms grown in Nigeria was analyzed using RAPD makers. The leaf samples for DNA extraction were collected from Nigerian Institute for Oil palm Resear ch (NIFOR) date palm experimental substation Dutse, Jigawa State, Nigeria.DNA was extracted from young leaves for PCR Amplification employing DNeasy Mini Kit. Five RAPD selected primers were employed to determine genetic variation among the ten date palms genotypes and also to test the effectiveness of RAPD primers as a genetic maker. Polymorphism was observed among all the date palms using the RAPD primers. Cluster analysis using sequence alignment was done to generated dendrogram verifying relationship among the ten studied date palms, with an average similarity matrix range of 0.00 to 0.85. This study has helped to design molecular markers such as RAPD that are suitable in the characterization of Nigerian date palm collection.

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“…A similar result was reported by Bekheet et al [16] in their study on biochemical and molecular approaches to sex determination in date palm.…”

Section: Resultssupporting

confidence: 89%

Molecular Characterization of some Date palms in Nigeria Using RAPD Markers

2015

J App Biol Biotech

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“…In fact, the ISSR-marker approach was successfully applied in a previous study on Carica papaya where it was reported to have led to the identification of (GATA)n as a sex related marker (Parasnis et al, 1999). Several other studies have, on the other hand, reported on the convenience of the RAPD method for sex determination (Bekheet et al, 2008). The latter was also applied for other species such Myristica fragrans Houtt.…”

Section: Discussionmentioning

confidence: 99%

“…In this context, RAPD markers were employed by Corniquel and Mercier (1994) for Date palm cultivar identification. This technique was also used by Bekheet et al (2008) for early identification of sex type in Date palm. Such markers, however, lack reproducibility.…”

Section: Introductionmentioning

confidence: 99%

Investigation of genetic variability related to the in vitro floral hermaphrodism induction in Date palm (Phnix dactylifera L.)

Masmoudi-Allouche¹,

Gargouri-Bouzid²,

Kriaâ³

et al. 2011

Afr. J. Biotechnol.

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This paper reports on a molecular analysis study conducted on Date palm flowers from the Deglet Nour cultivar to investigate putative genetic variability related to the in vitro floral hermaphrodism induction. Natural male and female as well as hermaphrodite ones that were produced in vitro through the hormonal treatment of female flowers were submitted to ISSR-PCR analysis. Microsatellite based amplification (ISSR) was applied on genomic DNA from inflorescences taken at different periods of hormonal treatment corresponding to the various deviation stages to search for putative variations that may have occurred on the initial genome due to the application of plant growth regulators. Several amplification bands were purified, cloned, and sequenced. The results revealed that hormonal treatment entailed no detectable genetic variation in the treated Date palm flowers. Two of the selected and ISSR-PCR amplified DNA fragments showed however, possible links with flowering regulation. The findings indicate that these sequences are potential candidate gene markers that may enhance our understanding of flower development and sex identification in this species.

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“…HRM tiene varias ventajas sobre otras tecnologías: es más simple, flexible, es no destructivo, de alta sensibilidad, específico, rentable y muy preciso (Kafkas et al, 2015;Simko, 2016), al ser un proceso continuo, en un solo análisis en el termociclador se pueden genotipar las plantas, sin necesidad de hacer la etapa de la electroforesis y, así, ahorrar tiempo y recursos. Además, estos marcadores no se ven afectados por las condiciones ambientales en las que crecen las plantas ni por la epistasis, por lo que la predicción del sexo se puede realizar en cualquier etapa de desarrollo del crecimiento de las plantas (Bekheet et al, 2007;Bhargava & Srivastava, 2019).…”

Section: Introductionunclassified

Análisis de curvas de fusión de alta resolución para identificar el sexo en papaya (Carica papaya L.)

Barrantes-Santamaría

2022

Agron. Mesoam.

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Introducción. El sexado de las plantas de papaya (Carica papaya L.) en plantaciones comerciales se suele hacer de forma visual una vez que la planta emite la flor. También existen procedimientos biotecnológicos como la Reacción en Cadena de la Polimerasa (PCR), utilizados para detectar a edades tempranas el sexo de la papaya, sin embargo, ambos métodos mencionados presentan una serie de inconvenientes entre ellos los falsos positivos. Objetivo. Identificar el sexo en plántulas de papaya con marcadores SNP (Single Nucleotide Polymorphism) de alto grado de precisión. Materiales y métodos. La investigación se realizó en la Estación Experimental Agrícola Fabio Baudrit Moreno de la Universidad de Costa Rica, durante los meses de febrero a octubre del año 2020. Se evaluaron ocho marcadores SNP en plantas de papaya de sexo conocido mediante la técnica HRMA (siglas en inglés: High Resolution Melting Analysis), se escogió el mejor y se reevaluó en dos poblaciones del programa de mejora genética de este cultivo, las plantas sexadas se sembraron en campo para comparar el sexado molecular con el real en campo. Resultados. Varios de los marcadores SNP evaluados fueron polimórficos y podrían ser usados para identificar el sexo en la papaya, sin embargo, el marcador CpSERK_HRM_34704 fue el que se ajustó más a los criterios de selección. Conclusión. Para el caso del híbrido de papaya Pococí y poblaciones de mejora emparentadas con el mismo, el marcador CpSERK_HRM_34704 mostró un 100 % de precisión entre le sexado molecular de las plántulas en el almácigo con la técnica HRM y el sexo real de las mismas plantas sembradas en el campo.

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In Vitro Differentiation of Zygotic Lines of Date Palm: Biochemical and Molecular Approaches to Sex Determination

Cited by 4 publications

References 0 publications

Molecular Characterization of some Date palms in Nigeria Using RAPD Markers

Molecular Characterization of some Date palms in Nigeria Using RAPD Markers

Investigation of genetic variability related to the in vitro floral hermaphrodism induction in Date palm (Phnix dactylifera L.)

Análisis de curvas de fusión de alta resolución para identificar el sexo en papaya (Carica papaya L.)

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