In vitro differentiation of human mesenchymal stem cells on Ti6Al4V surfaces

Tognarini, Isabella; Sorace, Sabina; Zonefrati, Roberto; Galli, Gianna; Gozzini, Alessia; Sala, Sílvia Carbonell; Thyrion, Giorgia Donata Zappoli; Carossino, Anna Maria; Tanini, Annalisa; Mavilia, Carmelo; Azzari, Chiara; Sbaiz, Fausto; Facchini, Alessandro; Capanna, Rodolfo; Brandi, Maria Luisa

doi:10.1016/j.biomaterials.2007.10.043

Cited by 43 publications

(35 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Various cellular sources, such as MCT3T3 osteogenic cell lines, mature osteoblasts, and MSCs, have been employed to evaluate the biocompatibility of titanium materials in numerous studies 4,5,8,[11][12][13]22,23) . Osteoblasts exist only in the internal surface of cortical bone, and their quantity and proliferation ability are limited.…”

Section: Discussionmentioning

confidence: 99%

“…Mesenchymal stem cells, on the other hand, play a more important role in osseointegration by continuously migrating, proliferating, and differentiating into osteoblasts. Therefore, MSCs have been suggested to be a good candidate for the evaluation of titanium materials' biocompatibility in cell attachment, proliferation, and differentiation stuides 22) . In the present study, SEM was used to evaluate the attachment of MSCs to titanium surface.…”

Section: Discussionmentioning

confidence: 99%

“…However, AMSCs were also reported to be a good candidate for bone tissue engineering and had shown similar capability in differentiating into mature osteoblasts 22,25) . In the present study, qRT-PCR analysis revealed a higher level of osteogenic commitment in BMSCs than in AMSCs, with respect to the expression levels of Runx2, Osterix, ALP, and OC on MAO, UV-2h, and UV-6h surfaces.…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, MSCs are the preferred cellular source for testing cell and material interactions 11,22,23) . Bone marrow-derived mesenchymal stem cells (BMSCs) are commonly used in bone tissue engineering and testing of titanium materials.…”

Section: Introductionmentioning

confidence: 99%

“…Recently, adipose-derived mesenchymal stem cells (AMSCs) were suggested to be a more convenient source of mesenchymal cells than BMSCs in terms of their abundance and accessibility. Although AMSCs were commonly thought to have less osteogenic potential than BMSCs 24) , it has been reported that when induced to osteogenic differentiation 22) , AMSCs could produce a calcified Ultraviolet irradiation enhanced bioactivity and biological response of mesenchymal stem cells on micro-arc oxidized titanium surfaces extracellular matrix 25,26) -hence exhibiting comparable osteogenic potential as BMSCs. Unfortunately, little data is available on testing the osteogenic potential of AMSCs on modified titanium surfaces.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Ultraviolet irradiation enhanced bioactivity and biological response of mesenchymal stem cells on micro-arc oxidized titanium surfaces

Huang

et al. 2015

Dent. Mater. J.

View full text Add to dashboard Cite

This present study investigated the effect of ultraviolet (UV) irradiation on bioactivity of micro-arc oxidized (MAO) titanium surface in vitro by cell culture medium immersion test and interactions with rat-derived mesenchymal stem cells (MSCs). UV-irradiated MAO surface exhibited no obvious changes in surface roughness, morphology, and phase composition when compared with MAOonly surface. However, in cell culture medium immersion test, markedly more bone-like apatite was formed on UV-modified samples than on MAO sample. Rat bone marrow-and adipose tissue-derived MSCs cultured on UV-modified samples displayed accelerated attachment, significant higher levels of alkaline phosphatase (ALP) activity, and up-regulated osteogenesis-related mRNA expression than MAO sample. XPS results provided direct evidence that the amount of basic hydroxyl groups increased with UV irradiation time, which could be one of the key mechanisms underlying their improved bioactivity.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Ultraviolet irradiation enhanced bioactivity and biological response of mesenchymal stem cells on micro-arc oxidized titanium surfaces

Huang

et al. 2015

Dent. Mater. J.

View full text Add to dashboard Cite

show abstract

In vitro osteoblastic differentiation of human mesenchymal stem cells and human dental pulp stem cells on poly‐L‐lysine‐treated titanium‐6‐aluminium‐4‐vanadium

Galli

Benedetti

Bongio

et al. 2011

J Biomedical Materials Res

View full text Add to dashboard Cite

Three-dimensional (3D) titanium-6-aluminium-4-vanadium (Ti6Al4V) is a widely used biomaterial for orthopedic prosthesis and dental implants; thanks to its very high-mechanical strength and resistance to corrosion. Human mesenchymal stem cells (hMSCs) and dental pulp stem cells (hDPSCs) are responsible for bone regeneration following colonization of prosthesis or dental implants. Both hMSCs and hDPSCs have lower ability to colonize this biomaterial in comparison with tissue culture-treated plastic. Both hMSCs and hDPSCs show lack of focal adhesion kinase (FAK) activation when grown on Ti6Al4V. This signal is restored in the presence of poly-L-lysine (poly-L-lys). Poly-L-lys has been used as part of organoapatite or together with zinc and calcium ions. Our results suggest that poly-L-lys alone induces FAK activation through β1-INTEGRIN, because the presence of β1-INTEGRIN blocking antibody avoided FAK autophosphorylation. Presence of poly-L-lys also increases expression of osteoblastic differentiation marker genes in hMSCs and hDPSCs grown on Ti6Al4V.

show abstract

Hierarchical titanium surface textures affect osteoblastic functions

Zhao

Cao

et al. 2011

J Biomedical Materials Res

View full text Add to dashboard Cite

This study investigated the surface characteristics and in vitro cytocompatibility of hierarchical textured titanium surfaces with nanograins and microroughness, produced by surface mechanical attrition treatment (SMAT). The surface characteristics were evaluated by scanning electron microscopy, X-ray diffraction, transmission electron microscopy, contact angle, and surface energy measurements. The in vitro cytocompatibility of the SMAT processed surfaces (hereafter Ti-SMAT surfaces) were assessed in terms of cellular attachment, morphology, viability, alkaline phosphatase (ALP) activity, and mRNA gene expression. Two other titanium surfaces were compared: well-polished Ti6Al4V surfaces (hereafter Ti-polish surfaces) and thermally sprayed rough surfaces (hereafter Ti-spray surfaces). The Ti-SMAT surfaces showed a higher hydrophilicity and increased surface energy compared with the Ti-polish and Ti-spray surfaces. Consequently, these Ti-SMAT surfaces demonstrated enhancement of cell attachment, spreading, viability, and ALP activity. Reverse transcriptase polymerase chain reaction (RT-PCR) analysis showed significantly higher ALP activity and stronger expression of mRNA levels of key osteoblast genes in cells grown on the Ti-SMAT surfaces than the other two surfaces. These results reveal a synergic role played by nanostructure and microtopography in osteoblastic functions and demonstrate the more promising cytocompatibility of the hierarchical textured surfaces. It is suggested that the SMAT process may provide a novel method of surface modification to the currently available metallic biomaterials.

show abstract

In vitro differentiation of human mesenchymal stem cells on Ti6Al4V surfaces

Cited by 43 publications

References 37 publications

Ultraviolet irradiation enhanced bioactivity and biological response of mesenchymal stem cells on micro-arc oxidized titanium surfaces

Ultraviolet irradiation enhanced bioactivity and biological response of mesenchymal stem cells on micro-arc oxidized titanium surfaces

In vitro osteoblastic differentiation of human mesenchymal stem cells and human dental pulp stem cells on poly‐L‐lysine‐treated titanium‐6‐aluminium‐4‐vanadium

Hierarchical titanium surface textures affect osteoblastic functions

Contact Info

Product

Resources

About