In vitro detection of small molecule metabolites excreted from cancer cells using a Tenax TA thin-film microextraction device

Nozoe, Takuma; Goda, Shigemi; Selyanchyn, Roman; Wang, Tao; Nakazawa, Kimitaka; Hirano, Takeshi; Matsui, Hidetaka; Lee, Seung Woo

doi:10.1016/j.jchromb.2015.04.016

Cited by 20 publications

(9 citation statements)

References 26 publications

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“…Benzaldehyde is a compound found in saliva, hair, and fingernails samples [37]; however, its origin can be exogenous, including gasoline vehicle emissions [38]. (E)-2-octenal and (E)-2-nonenal are expected to be biomarkers of inflammation and/or other carcinogenesis associated processes since they are identified in the headspace of human epithelial cervical carcinoma cells [39]. (E)-2-nonenal is also found as the cucumber odor in the cucumber/farinaceous subgroup of mushrooms with farinaceous odors [40].…”

Section: Resultsmentioning

confidence: 99%

VOC Profiles of Saliva in Assessment of Halitosis and Submandibular Abscesses Using HS-SPME-GC/MS Technique

et al. 2019

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Halitosis and submandibular abscesses are examples of mouth-related diseases with the possible bacterial origin. Salivary volatile organic compounds (VOCs) are potential biomarkers of them, once they can be addressed as metabolites of bacterial activity. Healthy patients (n = 15), subjects with submandibular abscesses located in fascial deep space (n = 10), and subjects with halitosis (n = 5) were enrolled in the study. Saliva samples were subjected to headspace solid-phase microextraction (HS-SPME) and gas chromatography coupled to mass spectrometry (GC/MS) analysis. A total number of 164 VOCs was detected by the developed methodology, 23 specific for halitosis and 41 for abscess. Halitosis’ profiles were characterized by a larger number of sulfur compounds, while for abscess they had a higher variety of alcohols, aldehydes, and hydrocarbons—biomarkers of inflammatory processes. Principal components analysis allowed visualization of clusters formed according to the evaluated conditions. Kruskal-Wallis test indicated that 39 VOCs presented differentiated responses between the studied groups, with statistical relevance (p < 0.05). Random forest was applied, and a prediction model based on eight VOCs (2-butanone, methyl thioacetate, 2-methylbutanoic acid, S-methyl pentanethioate, dimethyl tetrasulfide, indolizine, pentadecane, and octadecanal) provided 100% of sensitivity, 82% of specificity, and 91% of balanced accuracy, indicating the specific presence of submandibular abscess.

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Section: Resultsmentioning

confidence: 99%

VOC Profiles of Saliva in Assessment of Halitosis and Submandibular Abscesses Using HS-SPME-GC/MS Technique

et al. 2019

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“…The total analysis took 24.17 min to obtain the results. The MS analyses were done in full scan mode (TIC mode) with the scan range between 40 to 200 a.m.u and the electron impact ionization was done at 70eV to separate the compounds [ 30 ].…”

Section: Methodsmentioning

confidence: 99%

A study on volatile organic compounds emitted by in-vitro lung cancer cultured cells using gas sensor array and SPME-GCMS

et al. 2018

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BackgroundVolatile organic compounds (VOCs) emitted from exhaled breath from human bodies have been proven to be a useful source of information for early lung cancer diagnosis. To date, there are still arguable information on the production and origin of significant VOCs of cancer cells. Thus, this study aims to conduct in-vitro experiments involving related cell lines to verify the capability of VOCs in providing information of the cells.MethodThe performances of e-nose technology with different statistical methods to determine the best classifier were conducted and discussed. The gas sensor study has been complemented using solid phase micro-extraction-gas chromatography mass spectrometry. For this purpose, the lung cancer cells (A549 and Calu-3) and control cell lines, breast cancer cell (MCF7) and non-cancerous lung cell (WI38VA13) were cultured in growth medium.ResultsThis study successfully provided a list of possible volatile organic compounds that can be specific biomarkers for lung cancer, even at the 24th hour of cell growth. Also, the Linear Discriminant Analysis-based One versus All-Support Vector Machine classifier, is able to produce high performance in distinguishing lung cancer from breast cancer cells and normal lung cells.ConclusionThe findings in this work conclude that the specific VOC released from the cancer cells can act as the odour signature and potentially to be used as non-invasive screening of lung cancer using gas array sensor devices.

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“…For instance , straight-chained saturated aldehydes (e.g. n-hexanal (CAS 66-25-1) and n-octanal (CAS 922-63-4)) exhibit higher concen-trations in breath gas of lung cancer patients as compared to healthy volunteers [27, 28, 80, 81] or blood [82] of lung cancer patients as compared to healthy volunteers, whereas cancer cells have been shown to metabolize these compounds [59, 64, 65, 83-85]. The contrary profiles for several aldehydes in the breath gas samples and the headspace of transformed cell lines may be related to differences in the expre-ssion of aldehyde dehydrogenase (ALDH) isoforms (see section 4.2 below) or hypoxic conditions in the cultures under in vitro conditions, not observed in vivo (see section 4.3 below).…”

Section: Compounds Appearing In the Head-space Of Cultured Human Cellsmentioning

confidence: 99%

A Compendium of Volatile Organic Compounds (VOCs) Released By Human Cell Lines

Filipiak

Mochalski

Filipiak

et al. 2016

CMC

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Volatile organic compounds (VOCs) offer unique insights into ongoing biochemical processes in healthy and diseased humans. Yet, their diagnostic use is hampered by the limited understanding of their biochemical or cellular origin and their frequently unclear link to the underlying diseases. Major advancements are expected from the analyses of human primary cells, cell lines and cultures of microorganisms. In this review, a database of 125 reliably identified VOCs previously reported for human healthy and diseased cells was assembled and their potential origin is discussed. The majority of them have also been observed in studies with other human matrices (breath, urine, saliva, feces, blood, skin emanations). Moreover, continuing improvements of qualitative and quantitative analyses, based on the recommendations of the ISO-11843 guidelines, are suggested for the necessary standardization of analytical procedures and better comparability of results. The data provided contribute to arriving at a more complete human volatilome and suggest potential volatile biomarkers for future validation. Dedication: This review is dedicated to the memory of Prof. Dr. Anton Amann, who sadly passed away on January 6, 2015. He was motivator and motor for the field of breath research.

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In vitro detection of small molecule metabolites excreted from cancer cells using a Tenax TA thin-film microextraction device

Cited by 20 publications

References 26 publications

VOC Profiles of Saliva in Assessment of Halitosis and Submandibular Abscesses Using HS-SPME-GC/MS Technique

VOC Profiles of Saliva in Assessment of Halitosis and Submandibular Abscesses Using HS-SPME-GC/MS Technique

A study on volatile organic compounds emitted by in-vitro lung cancer cultured cells using gas sensor array and SPME-GCMS

A Compendium of Volatile Organic Compounds (VOCs) Released By Human Cell Lines

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