In-Vitro Degradation Test for Screening of Biomaterials

Welling, C. E.; Schwengler, Helge; Strahl, Brian D.

doi:10.1007/978-3-642-77563-5_3

Cited by 2 publications

(2 citation statements)

References 4 publications

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“…It is pertinent to note, however, that while FTIR and collagenase digestion assays are useful techniques to monitor the stability of the hydrogels, for a complete assessment of long-term storage, complementary methods (such as weight loss, mechanical properties including stress/strain curves, surface changes using SEM, contact angle and analysis of degradation solutions, etc.) would be necessary [31] , [32] . Nevertheless, our results indicated that CLP-PEG hydrogels remained sufficiently stable when stored for over 12 months.…”

Section: Discussionmentioning

confidence: 99%

Short peptide analogs as alternatives to collagen in pro-regenerative corneal implants

et al. 2018

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Section: Discussionmentioning

confidence: 99%

Short peptide analogs as alternatives to collagen in pro-regenerative corneal implants

et al. 2018

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“…The primary challenge in developing biodegradable hydrogels for drug delivery lies in accurately measuring their degradation over time, while also simultaneously being able to evaluate the drug-release kinetics. Standard approaches to assess the biodegradation of hydrogels are either by volume or mass loss, the latter of which is more common [13][14][15]. When assessing mass loss, researchers measure the weight of the dried gel at a specified time point [14,15].…”

Section: Introductionmentioning

confidence: 99%

A Rapid Screening Platform for Simultaneous Evaluation of Biodegradation and Therapeutic Release of an Ocular Hydrogel

Ho,

Phan,

Garg

et al. 2023

Pharmaceutics

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This study attempts to address the challenge of accurately measuring the degradation of biodegradable hydrogels, which are frequently employed in drug delivery for controlled and sustained release. The traditional method utilizes a mass-loss approach, which is cumbersome and time consuming. The aim of this study was to develop an innovative screening platform using a millifluidic device coupled with automated image analysis to measure the degradation of Gelatin methacrylate (GelMA) and the subsequent release of an entrapped wetting agent, polyvinyl alcohol (PVA). Gel samples were placed within circular wells on a custom millifluidic chip and stained with a red dye for enhanced visualization. A camera module captured time-lapse images of the gels throughout their degradation. An image-analysis algorithm was used to translate the image data into degradation rates. Simultaneously, the eluate from the chip was collected to quantify the amount of GelMA degraded and PVA released at various time points. The visual method was validated by comparing it with the mass-loss approach (R = 0.91), as well as the amount of GelMA eluted (R = 0.97). The degradation of the GelMA gels was also facilitated with matrix metalloproteinases 9. Notably, as the gels degraded, there was an increase in the amount of PVA released. Overall, these results support the use of the screening platform to assess hydrogel degradation and the subsequent release of entrapped therapeutic compounds.

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In-Vitro Degradation Test for Screening of Biomaterials

Cited by 2 publications

References 4 publications

Short peptide analogs as alternatives to collagen in pro-regenerative corneal implants

Short peptide analogs as alternatives to collagen in pro-regenerative corneal implants

A Rapid Screening Platform for Simultaneous Evaluation of Biodegradation and Therapeutic Release of an Ocular Hydrogel

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