In Vitro Degradation, Hemocompatibility, and Cytocompatibility of Nanostructured Absorbable Fe–Mn–Ag Alloys for Biomedical Application

Abstract

The addition of noble elements such as Ag was shown as a successful method to accelerate the corrosion rate of absorbable Fe-based alloys. One major concern of Ag addition is its effect on hemocompatibility and biocompatibility. In this study, in vitro degradation and surface analysis of Fe-30Mn-xAg (x= 0, 1 and 3 wt.%) alloys as well as their effects on hemocompatibility and cell viability of human umbilical vein endothelial cells (HUVECs) were investigated. The static degradation rate of the alloys was 4.97, 4.69 and 4.49 mg/cm 2 for Fe-30Mn, Fe-30Mn-1Ag, and Fe-30Mn-3Ag, respectively. The surface analysis after degradation showed that γ-FeOOH was formed on Fe-30Mn-3Ag, while α-FeOOH was more dominant on Fe-30Mn and Fe-30Mn-1Ag. As γ-FeOOH is more soluble than α-FeOOH, it assists further degradation of Fe-30Mn-3Ag alloy. The high amount of Ag, induced hemolysis ratio however, inhibited coagulation by decreasing the platelet adhesion. Fe-30Mn-1Ag and Fe-30Mn-3Ag alloys shown improved cell viability compared to that of Fe-Mn alloy. Shear yield strength and shear elastic modulus of the samples after immersion tests were increased while the ultimate shear strength was not affected. Based on acceptable hemolysis rate, low platelet adhesion, acceptable cell viability, and appropriate mechanical properties after degradation Fe-30Mn-1Ag can be considered as a suitable blood-contacting Fe-based absorbable alloy.

Cocktail of CuO, ZnO, or CuZn Nanoparticles and Antibiotics for Combating Multidrug-Resistant Pseudomonas aeruginosa via Efflux Pump Inhibition

“…Overall, all the tested NPs exerted hemolytic activity only at high concentrations. These results show that at sub-inhibitory concentrations, especially concentrations used in all the previous experiments, none of the tested compositions caused hemolysis that exceeds the criteria of hemocompatible biomaterials (5%). , Similar results about the hemocompatibility of CuO@PEG and CuZn@TrEG NPs were obtained from previous findings. , …”

“…These results show that at sub-inhibitory concentrations, especially concentrations used in all the previous experiments, none of the tested compositions caused hemolysis that exceeds the criteria of hemocompatible biomaterials (5%). 67,68 Similar results about the hemocompatibility of CuO@PEG and CuZn@TrEG NPs were obtained from previous findings. 69,70 ■ CONCLUSIONS Considering the alarming increase of AMR in P. aeruginosa, a combination therapy based on the association of antibiotics with NPs seems to be a promising approach to combat MDR P. aeruginosa infections.…”

FeMn with Phases of a Degradable Ag Alloy for Residue-Free and Adapted Bioresorbability

“…However, further aspects have to be considered to design such material. The various elements and chemical compounds in the human body result in the formation of various corrosion products, forming a multilayer structure [ 36 , 43 , 44 ]. These structures might affect further degradation.…”

Electrochemical behavior, biocompatibility and mechanical performance of biodegradable iron withPEIcoating

“…Intracellular erythrocyte release (hemolysis) can be caused by different factors including erythrocyte interactions with metal ions or other chemicals but also electrical forces. 55 Therefore, the main interaction between PEI, known for its polycationic character, and red blood cells (RBCs) may run through the interaction with the negative surface charge of the RBCs. Material with a hemolysis rate under 5% is considered highly hemocompatible 21 whereas the samples with a hemolysis rate between 5% and 10% are compatible.…”