In Vitro Decidualization of Rat Endometrial Stromal Cells.

Matsumoto, Kenji; Watanabe, Ryoji; Oozono, Shinji; Kubota, Kaiyu; Nishimura, Kyouhei; Soh, Tomoki; Yamauchi, Nobuhiko; Hattori, Masa Aki

doi:10.1093/biolreprod/78.s1.285

Cited by 10 publications

(15 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…UESCs were isolated from Per2-dLuc transgenic rats on D4.5 as reported previously (39,52 The uterine lumina were filled with PBS containing 0.1% collagenase and incubated at 37°C for 1 h in a shaking water bath. The harvested cells were washed three times with fresh DMEM-F12 and seeded onto 35-mm collagen-coated dishes at the density of 3 ϫ 10 5 cells/dish with 2 ml of culture medium (phenol red-free DMEM-F12 supplemented with 10% charcoal-treated fetal bovine serum and 1ϫ PBS).…”

Section: Animalsmentioning

confidence: 99%

See 1 more Smart Citation

Removal of Rev-erbα inhibition contributes to the prostaglandin G/H synthase 2 expression in rat endometrial stromal cells

Isayama

Zhao

Chen

et al. 2015

American Journal of Physiology-Endocrinology and Metabolism

Self Cite

View full text Add to dashboard Cite

The rhythmic expression of clock genes in the uterus is attenuated during decidualization. This study focused on Ptgs2, which is essential for decidualization, as a putative clock-controlled gene, and aimed to reveal the functions of clock genes in relation to Ptgs2 during decidualization. We compared the transcript levels of clock genes in the rat uterus on days 4.5 (D4.5) and 6.5 of pregnancy. The transcript levels of clock genes (Per2, Bmal1, Rorα, and Rev-erbα) had decreased at implantation sites on day 6.5 (D6.5e) compared with those on D4.5, whereas Ptgs2 transcripts had increased on D6.5e. Similar observations of Rev-erbα and Ptgs2 were also obtained in the endometrium on D6.5e by immunohistochemistry. In the decidual cells induced by medroxyprogesterone and 2-O-dibutyryl-cAMP, the rhythmic expression levels of clock genes were attenuated, whereas Ptgs2 transcription was induced. These results indicate that decidualization causes the attenuation of clock genes and the induction of Ptgs2. Furthermore, in the experiment of Bmal1 siRNA, the rhythmic expression of clock genes and Ptgs2 was attenuated by the siRNA. Transcript levels of Ptgs2 and prostaglandin (PG)E₂ production were increased by treatment with the Rev-erbα antagonist, suggesting the contribution of the nuclear receptor Rev-erbα to Ptgs2 expression. Moreover, Rev-erbα knockdown enhanced the induction of Ptgs2 transcription and PGE₂ production by forskolin. Chromatin immunoprecipitation-PCR analysis revealed that Rev-erbα could directly bind to a proximal RORE site of Ptgs2. Collectively, this study demonstrates that the attenuation of the circadian clock, especially its core component Rev-erbα, contributes to the induction of Ptgs2 during decidualization.

show abstract

Section: Animalsmentioning

confidence: 99%

“…Confluent UESCs were further cultured for 6 days in DMEM-F12 supplemented with 100 nM medroxyprogesterone acetate (MPA, Sigma-Aldrich), 0.5 mM 2-O-dibutyryl cAMP (db-cAMP, Sigma-Aldrich), 1ϫ AA, 1ϫ ITS, 0.1% BSA, as previously described (39). The differentiating status was revealed by expression of the Prl8a2 gene (41).…”

Section: Animalsmentioning

confidence: 99%

Removal of Rev-erbα inhibition contributes to the prostaglandin G/H synthase 2 expression in rat endometrial stromal cells

Isayama

Zhao

Chen

et al. 2015

American Journal of Physiology-Endocrinology and Metabolism

Self Cite

View full text Add to dashboard Cite

show abstract

“…Rat endometrial stromal cells (RES) were isolated and cultured as previously described (Matsumoto et al, 2009). RES were isolated from uterine horns of pregnant rats (day 5), which were dissected longitudinally to expose the uterine lumen and incubated with 0.5% trypsin (Invitrogen Corporation, Carlsbad, CA, USA) at 4°C for 1 h. The partially digested tissues were then washed twice in Hank's balanced salt solution (HBSS) and placed in HBSS containing 1 mM EDTA, DNase I (200 U/ml) (Roche Diagnostics GmbH, Mannhein, Germany) and 0.05% trypsin at 37°C for 30 min.…”

Section: Isolation Of Stromal Cells and Induction Of Decidualization mentioning

confidence: 99%

Anandamide restricts uterine stromal differentiation and is critical for complete decidualization

Fonseca

Correia-da-Silva

Teixeira

2015

Molecular and Cellular Endocrinology

View full text Add to dashboard Cite

“…Decidualization of RES was induced by incubating confluent cells in a medium containing 100 nM medroxyprogesterone acetate (MPA) and 0.5 mM dibutyryl-cyclic adenosine monophosphate (db-cAMP) as described previously (Matsumoto et al 2009). The culture medium was changed in every 2 days with continuous supplementation of these treatments.…”

Section: In Vitro Decidualization (Ivd)mentioning

confidence: 99%

“…To clarify the effect of decidualization on the expression of PPARα and PPARβ/δ, we used the IVD system of RES according to our previous method (Matsumoto et al 2009). In vitro-cultured RES, derived from the uterus during the implantation period (day 4.5 of pregnancy), consisted of a monolayer of spindle-like and fibroblast-like cells.…”

Section: Ivd On the Expressions Of Pparα And Pparβ/δ In Resmentioning

confidence: 99%

Expression of peroxisome proliferator-activated receptor isoforms in the rat uterus during early pregnancy

et al. 2011

Self Cite

View full text Add to dashboard Cite

Peroxisome proliferator-activated receptors (PPARs) play an important role in different compartments of the female reproductive system in rodents and humans. However, expressional profiles and physiological functions of PPARs in the endometrium prior to the placentation are not well understood. In this study, we determined expressional profiles of the PPARs during early pregnancy. Immunocytochemistry revealed that both PPARα and PPARβ/δ were strongly detected in the endometrial stroma on days 4.5-6.5 of pregnancy, which is just a starting time of implantation. Delayed implantation animal model showed that the expressions of PPARα and PPARβ/δ occurred after the initiation of implantation in the endometrial stroma. Moreover, an in vitro decidualization model further revealed that the expression of PPARα increased in the cultured rat endometrial stromal cells at 24 h after the decidualization treatment, but the expression of PPARβ/δ was delayed and increased at 48 h after the treatment. PPARγ was expressed in the endometrial stroma and its expression decreased significantly at 2.5 days post-coitum and maintained a low level of expression during the period of implantation. These results indicate that PPARα is expressed and induced by the initiation of implantation, prior to the expression of PPARβ/δ in decidualized endometrium. Increasing expression of PPARγ during fertilization and its decline during the period of implantation further suggest that PPARs may play important roles during early pregnancy.

show abstract

In Vitro Decidualization of Rat Endometrial Stromal Cells.

Cited by 10 publications

References 0 publications

Removal of Rev-erbα inhibition contributes to the prostaglandin G/H synthase 2 expression in rat endometrial stromal cells

Removal of Rev-erbα inhibition contributes to the prostaglandin G/H synthase 2 expression in rat endometrial stromal cells

Anandamide restricts uterine stromal differentiation and is critical for complete decidualization

Expression of peroxisome proliferator-activated receptor isoforms in the rat uterus during early pregnancy

Contact Info

Product

Resources

About