In vitro cytotoxity of silver: implication for clinical wound care

Poon, Vincent Kwok Man; Burd, Andrew

doi:10.1016/j.burns.2003.09.030

Cited by 455 publications

(326 citation statements)

References 31 publications

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“…Based on the MTT data one could expect approximately 100% cell death, which was supported by light microscope pictures. This is also supported by previous work showing that silver ions are toxic to skin cells in vitro (Barnea, Weiss & Gur, 2010;Burd et al, 2007; Paddle- Ledinek, Nasa & Cleland, 2006;Poon & Burd, 2004).…”

Section: Discussionsupporting

confidence: 86%

Producing ultrapure wood cellulose nanofibrils and evaluating the cytotoxicity using human skin cells

Nordli

Chinga-Carrasco²,

Rokstad

et al. 2016

Carbohydrate Polymers

125

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 Cellulose nanofibril dispersion (50 µg/ml) did not affect the cytotoxicity or metabolic activity of Normal Human Dermal Fibroblasts and Human Epidermal Keratinocytes  Aerogels made of cellulose nanofibrils induced a reduction of metabolic activity by the fibroblasts and keratinocytes, but no significant cell death  Cytokine profiling measuring 27 cytokines revealed that the keratinocytes and fibroblasts did not induce cytokines upon direct exposure to the CNF materials. Due to the nano dimension of the CNFs, the aerogels had a high moisture-holding capacity (~7500%) 3 AbstractWood cellulose nanofibrils have been suggested as a potential wound healing material, but its utilization is limited by FDA requirements regarding endotoxin levels. In this study a method using sodium hydroxide followed by TEMPO mediated oxidation was developed to produce ultrapure cellulose nanofibrils (CNF), with an endotoxin level of 45 endotoxin units/g (EU/g) cellulose. Scanning transmission electron microscopy (S(T)EM) revealed a highly nanofibrillated structure (lateral width of 3.7±1.3 nm).Assessment of cytotoxicity and metabolic activity on Normal Human Dermal Fibroblasts and Human Epidermal Keratinocytes was done. CNF-dispersion of 50 g/ml did not affect the cells. CNF-aerogels induced a reduction of metabolic activity by the fibroblasts and keratinocytes, but no significant cell death. Cytokine profiling revealed no induction of the 27 cytokines tested upon exposure to CNF. The moisture-holding capacity of aerogels was relatively high (~7500%), compared to a commercially available wound dressing (~2500%),indicating that the CNF material is promising as dressing material for management of wounds with a moderate to high amount of exudate.

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Section: Discussionsupporting

confidence: 86%

Producing ultrapure wood cellulose nanofibrils and evaluating the cytotoxicity using human skin cells

Nordli

Chinga-Carrasco²,

Rokstad

et al. 2016

Carbohydrate Polymers

125

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“…Mean healing times were significantly (P,0.0001) shorter in saline-soaked dressing than silver sulfadiazine. This finding identical to other studies [3,[18][19][20][21]. Healin time was normal when silver sulfadiazine was used in spit-thickness donor sites but significantly slower in deeper wounds [3,21].…”

Section: Discussionsupporting

confidence: 89%

“…Adverse effects of silver sulfadiazine includes: 1-delayed wound healing is often observed clinically following the use of silver-containing topical antimicrobial agents [18,19]. Silver sulfadiazine cream might also slow down the proper healing mechanisms of the wound [20].…”

Section: Discussionmentioning

confidence: 99%

“…Understanding the characteristics of these products and dressings may enable them to be targeted more appropriately according to the specific requirements [29]. A word of caution, however, about extrapolating too directly from laboratory studies to clinical application, nevertheless, based on available evidence it is recommend at present that silver-based products should be avoided if possible as a topical antimicrobial strategy where rapidly proliferating keratinocytes are exposed as in donor sites, superficial partial thickness wounds and undifferentiated cultured keratinocyte applications [18,29]. The ultimate goal remains the choice of a product with superior profile of antimicrobial activity over cellular toxicity [28,29].the search for suitable topical burn therapy will continue to focus on preparations that can kill microbes and promote tissue regeneration while limiting tissue damage.…”

Section: Discussionmentioning

confidence: 99%

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A Comparative Study of the Burn Wound Healing Properties of Saline-Soaked Dressing and Silver Sulfadiazine in Rats

2010

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The purpose of this study was to further investigate that phenomenon and to explore the effect silver sulfadiazine on wound healing. Full-thickness burn wounds were created on the dorsum of Wistar albino rats under anesthesia. The wounds were treated with silver sulfadiazine and saline-soaked dressing for fourteen days, and then observed until healed. Wound surface area was measured each three days. Time to 50% and 90% healing was compared. No clinical infections occurred. Wound half-life and healing times were shortest in the saline-soaked group (P<0.0001) in full-thickness burns. Wound contraction was delayed by silver sulfadiazine. These data suggest that silver sulfadiazine retard burn wound healing. Infection control without delay of burn wound healing is most appealing and clinical trials are planned.

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“…Antiseptics in common use are all cytotoxic and so slow the healing process [150J. Silver also is cytotoxic [151J and can cause poisoning systemically when absorbed from wound dressings [151]. Honey, however, is not only not toxic, but actually stimulates the growth of cells involved in wound healing [152-154J and stimulates the production of the components of the extracellular matrix [155.…”

Section: Benefits Apart From Control Of Infection In Topical Treatmenmentioning

confidence: 99%

Honey: Antimicrobial Actions and Role in Disease Management

Molan

2008

New Strategies Combating Bacterial Infection

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The ancient treatment of dressing infected wounds wi.th honey is rapidly becoming re-established in professional medicine, especially where wounds are infected with antibiotic-resistant bacteria. This is because ofthe demonstrated sensitivity of such bacteria to the antibacterial activity ofhauey, which is not influenced by whether or not strains are resistant to antibiotics. Honey has been found to have a very broad spectrum of activity, but its potency of antibacterial activity can vary greatly. In most honeys the antibacterial activity is due to enzymatically produced hydrogen peroxide and thus the potency of its antibacterial activity can be decreased by catalase present in an open wound. Manuka honey has an antibacterial component derived from the plant source. Manuka honey with a quality-assured level of antibacterial activity is being used by companies marketing honey products for wound care that are registered with the medical regulatory authorities in various countries. Such honey can be diluted IO-fold or more and still completely inhibitthe usual wound-infecting species. There is a large amount of clinical evidence for the effectiveness of honey in clearing infection in wounds, and some clinical evidence of its effectiveness in treating other infections. Although the antibacterial potency of honey is insufficient to allow its use systemically, there are various clinical applications besides wound care in which it is used topically or where it does not get excessively diluted, such as for treatment of gastritis, enteritis, gingivitis, ophthalmological infections and bronchial infections. In most of these applications the anti-inflammatory activity of honey is of additional benefit in decreasing the inflammation resulting from infection. Additional clinical research is needed to provide better evidence of the effectiveness of honey in these therapeutic applications of honey.New Strategies CombClting Bacterial Infection. Edited by Iqbal Ahmad and Farrukh Aqii

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In vitro cytotoxity of silver: implication for clinical wound care

Cited by 455 publications

References 31 publications

Producing ultrapure wood cellulose nanofibrils and evaluating the cytotoxicity using human skin cells

Producing ultrapure wood cellulose nanofibrils and evaluating the cytotoxicity using human skin cells

A Comparative Study of the Burn Wound Healing Properties of Saline-Soaked Dressing and Silver Sulfadiazine in Rats

Honey: Antimicrobial Actions and Role in Disease Management

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