In vitro cytotoxicity and dentin permeability of HEMA

Bouillaguet, Serge; Wataha, John C.; Hanks, C. T.; Ciucchi, B.; Holz, J

doi:10.1016/s0099-2399(06)80141-x

Cited by 188 publications

(164 citation statements)

References 15 publications

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“…However, dentin thickness is known to dissipate the concentration of potential toxins over distance. 42 GA is used in many different processes as a fixative, cross-linking, and disinfecting agent. As is the case for EDC, GA participates in the construction of bioprosthetic heart valves, 26,43 inhibits proteases, 31 and modifies gelatins and other materials and tissues.…”

Section: Discussionmentioning

confidence: 99%

“…That is, the applied concentration rapidly falls the further the solute diffuses into dentin, so that by the time it reaches the other side of the dentin, its concentration has fallen by a 10-, 100-, or 1000-fold measure. 42 Finally, when the dentin surface is rinsed with water to stop inward diffusion by dilution, the solutes in dentin start to diffuse backwards out of the tubules, since there is more solute in the tubules than at the water rinsed surface. All of these processes protect the pulpal cells from solutes applied to dentin surfaces.…”

Section: Discussionmentioning

confidence: 99%

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Transdentinal Cytotoxicity of Carbodiimide (EDC) and Glutaraldehyde on Odontoblast-like Cells

Scheffel

Bianchi

Soares

et al. 2015

Operative Dentistry

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Carbodiimide (EDC) and glutaraldehyde are cross-linking agents capable of increasing resin-dentin bond durability. This study showed that these substances might be safely applied on acid-etched dentin since they have no transdentinal cytotoxic effects on cultured odontoblast-like cells. SUMMARYObjective: To evaluate the transdentinal cytotoxicity of three different concentrations of carbodiimide (EDC) or 5% glutaraldehyde (GA) on MDPC-23 cells.Methods: Seventy 0.4-mm-thick dentin disks obtained from human molars were adapted to artificial pulp chambers. MDPC-23 cells were seeded on the pulpal surface of the disks. After 48 hours, the occlusal dentin was acid-etched and treated for 60 seconds with one of the Results: EDC at all test concentrations did not reduce cell viability, while 5% GA did increase cell metabolism. Cell death by necrosis was not elicited by EDC or 5% GA. At the 24-hour period, 0.3 M and 0.5 M EDC reduced TP production by 18% and 36.8%, respectively. At seven days, increased TP production was observed in all groups. Collagen production at the 24-hour period was reduced when 0.5 M EDC was used. After seven days, no difference was observed among the groups. SEM showed no alteration in cell morphology or number, except in the hydrogen peroxide group.Conclusions: Treatment of acid-etched dentin with EDC or GA did not cause transdentinal cytotoxic effects on odontoblast-like cells.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Transdentinal Cytotoxicity of Carbodiimide (EDC) and Glutaraldehyde on Odontoblast-like Cells

Scheffel

Bianchi

Soares

et al. 2015

Operative Dentistry

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“…For example, HEMA can be released into the oral environment reaching the concentration of 8 mM 26) . Similarly, the detected quantity of TEGDMA reaching the pulp had a concentration of 4 mM 27) .…”

Section: Discussionmentioning

confidence: 99%

Effects of a dental adhesive on cell cycle regulatory proteins

et al. 2013

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Dental bonding agents may affect the cell cycle patterns and induce cell cycle arrest by blocking its progression. This study tested the cell cycle effects through cyclin-dependent kinase (cdc2) and Rb phosphorylation. Human lung fibroblasts (MRC5) were used for the experiments. The bonding agent tested was the total-etch XP bond. Extracts of the bonding agent were prepared and serial dilutions were tested. The effects of the bonding agent on cell survival, proliferation and DNA synthesis were tested by the SRB and BrdU assays. Analysis of cell cycle distribution was performed by flow cytometry. XP bond exhibited strong inhibition of DNA synthesis and after 48 h of exposure cells were accumulated in the G2/M phase. Cells exposed to the half maximal cell growth inhibitory concentration (IC 50 ) showed an increase in cdc2 kinase and Rb phosphorylation. The results most likely indicate mutagenic effect of the tested agent.

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“…HEMA is an amphoteric monomer that displaces water in dentin and is capable of diffusing rapidly through the dentin (Bouillaguet et al, 1996). It is also miscible with most of the monomers used in composites (Bouillaguet, 2004).…”

Section: Dental Polymersmentioning

confidence: 99%

“…It is also miscible with most of the monomers used in composites (Bouillaguet, 2004). Therefore, the possibility of other components of the adhesive system acting synergistically with HEMA to increase cytotoxicity needs to be investigated, especially because other relatively hydrophobic resinous composites are soluble in HEMA and may be carried through the dentin (Bouillaguet et al, 1996).…”

Section: Dental Polymersmentioning

confidence: 99%

Polymer Biocompatibility

Porto¹

2012

Polymerization

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In vitro cytotoxicity and dentin permeability of HEMA

Cited by 188 publications

References 15 publications

Transdentinal Cytotoxicity of Carbodiimide (EDC) and Glutaraldehyde on Odontoblast-like Cells

Transdentinal Cytotoxicity of Carbodiimide (EDC) and Glutaraldehyde on Odontoblast-like Cells

Effects of a dental adhesive on cell cycle regulatory proteins

Polymer Biocompatibility

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