In vitro Cytotoxic Effect of Lymphoid Cells from Mice Immunized with Allogeneic Tissue

Vainio, Tapani; Koskimies, Olli; Perlmann, Peter; Perlmann, Hedvig; Klein, George

doi:10.1038/204453a0

Cited by 41 publications

(15 citation statements)

References 7 publications

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“…This view has been substantiated by recent demonstrations that lymphoid cells (6)(7)(8)(9)(10)(11)(12)(13)(14)(15) and macrophages (16,17) procured from specifically immunized animals have a cytocidal effect on appropriate homologous "target" cells in vitro. It was previously reported that by the 6th to 7th day after grafting with homologous skiu, lymphocytes from the regional lymph nodes of mice or rats had acquired the capacity to injure foreign target renal cells in culture, and that neither isoimmune serum nor complement was required for this destructive reactivity (15).…”

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confidence: 62%

Quantitative Studies on the Behavior of Sensitized Lymphocytes in Vitro

Wilson

1965

The Journal of Experimental Medicine

163

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Numerous studies have implicated cells of the lymphoid series as agents responsible for the destruction of solid tissue homografts in vivo (1)(2)(3)(4)(5). This view has been substantiated by recent demonstrations that lymphoid cells (6-15) and macrophages (16, 17) procured from specifically immunized animals have a cytocidal effect on appropriate homologous "target" cells in vitro. It was previously reported that by the 6th to 7th day after grafting with homologous skiu, lymphocytes from the regional lymph nodes of mice or rats had acquired the capacity to injure foreign target renal cells in culture, and that neither isoimmune serum nor complement was required for this destructive reactivity (15). The present communication is concerned with the application of a sensitive, reproducible in vitro assay system to elucidate further quantitative aspects of the destruction of homologous target cells by sensitized lymphocytic cells. Particular attention has been paid to the influence of (a) variation in the ratio of attacking lymphoid cells to target ceils (b) the duration of contact with lymphoid cells and (c) the possible influence of the presence or absence of isoimmune serum on the cytocidal activities of sensitized lymphocytes. Materials and MethodsPrinciple of the Test System.--Rats were immunized to the foreign transplantation antigens of a homologous strain. Cell suspensions consisting of known numbers of lymphocytes prepared from the lymph nodes of normal, i.e. non-immune, or sensitized animals were then added to previously "seeded" monolayer cultures of knoWn numbers of target cells of donor strain origin. After a specified period of incubation, the number of target cells remaining in the

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confidence: 62%

Quantitative Studies on the Behavior of Sensitized Lymphocytes in Vitro

Wilson

1965

The Journal of Experimental Medicine

163

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“…If it is neces sary to study the cytocidal effect independently from the cytostatic effect, the method described in this report will be also applicable by use of target cells prelabeled with 3H-TdR (Vainio et al 1964), 1251UdR (Cohen et al 1971), and 3H-proline (Bean et al . 1973).…”

Section: Examinedmentioning

confidence: 99%

“…A majority of cell-mediated cytotoxic assays with radioisotopic techniques in vitro have been directed toward the cytocidal effect which is measured by the loss of prelabeled target cells from a culture surface (Vainio et al 1964; Jagarlamo ody et al 1971; Cohen et al 1971; Bean et al 1973) or the release of 51Cr (Brunner et al 1968). In addition, it has been recently shown that tumor cells are damaged by the cytostatic effect (Takasugi and Calderon et al 1974) as well as the cytocidal effect.…”

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confidence: 99%

A simple method of cell-mediated cytotoxic assay by postlabeling of tritiated thymidine.

Toh¹,

Kikuchi²

1977

Tohoku J. Exp. Med.

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The cytotoxic reaction of effector cells on target cells was performed on cover slips which were set in a petri dish so as to be interposed from the dish surface through the bases of the slide glasses. After incubation, the target cells remaining on the cover slips were pulse-labeled with 3H-TdR for 45 min. Subsequently, the cover slips were removed from the bases, and dipped in cold 5% TCA to be freed from non-incorporated isotopes. The residual target cells adhering on the cover slips were counted without being removed.By this assay system, the specific or non-specific cytotoxicity of lymphocytes or macrophages was easily demonstrated with reliability. -cell-mediated cytotoxic assay; 3H -thymidine; cover slip; lymphocyte; macrophage

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“…Several investigators, employing a variety of techniques, have demonstrated cell-killing effects of allogeneic lymphocytes (1)(2)(3)(4)(5). The cells serving as targets for the action of lymphocytes have been normal cells carrying different histocompatibility antigens, as well as transformed cells bearing new antigens, the so-called tumor transplantation antigens.…”

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confidence: 99%