In vitro cytotoxic activity of certain succulent plants against human colon, breast and liver cancer cell lines

Abdul-Hafeez, Essam Y.; Orabi, Mohamed A. A.; Ibrahim, Omer H. M.; Ilinskaya, О. N.; Karamova, Nazira S.

doi:10.1016/j.sajb.2020.02.023

Cited by 8 publications

(6 citation statements)

References 29 publications

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“…does not possess antitumor activity against A-549 [42] and with Abdul-Hafeez et alwho proved the low cytotoxic activity of S. trifasciata Prain. against colon, breast and hepatic cell lines [9]. On the other hand, S. suffruticosa N.E.Br.…”

Section: Discussionmentioning

confidence: 99%

Sansevieria: An evaluation of cytotoxic activity in reference to metabolomic and molecular docking studies

et al. 2020

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Sansevieria trifasciata Prain. and Sansevieria suffruticosa N.E.Br. were selected to evaluate their cytotoxic activity against colon (CACO2), lung (A-549) and liver (HepG-2) carcinoma cell lines. Results indicated that S. suffruticosa N.E.Br. showed a significant cytotoxic effect on CACO2 with IC50= 30.9 ± 0.72 μg/ml. Evaluating the phenolic and flavonoid contents, S. suffruticosa N.E.Br. has higher total phenolic and flavonoid contents than S. trifasciata Prain. The phenolic content was estimated using HPLC detecting the presence of apigenin-7-glucoside and cinnamic acid only in S. suffruticosa N.E.Br. with p-hydroxybenzoic acid and p-coumaric acid being the major compounds. While, catechin and kaempferol are the major compounds detected in S. trifasciata Prain. Phytochemical investigation of ethyl acetate fractions of both species results in isolation of chlorogenic acid, kaempferol, quercetin, and catechin from S. trifasciata Prain. and apigenin-7-glucoside and rutin from S. suffruticosa N.E.Br. Isolated compounds are identified using recent spectroscopic methods. All compounds are isolated for the first time from these species. Metabolomic profiling indicates the presence of phenolic acids, flavonoids and saponins in the alcoholic extracts of the two species. In addition, molecular docking study was done for further investigation of the possible targets involved in the cytotoxicity of the alcoholic extracts of the two Sansevieria species. The ability of some phenolic compounds to interact with EGFR cancer target site rationalizes the cytotoxic activity of S. suffruticosa N.E.Br. alcoholic extract as proven by their docking pattern and docking score.

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Section: Discussionmentioning

confidence: 99%

Sansevieria: An evaluation of cytotoxic activity in reference to metabolomic and molecular docking studies

et al. 2020

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“…Dracaena (Sansevieria) trifasciata is an important ethnomedicinal plant that is readily available at a very economical price in Pakistan. Literature associates anti-diabetic, anti-tumor, anti-oxidant, and anti-microbial activities with species of this genus thus corroborating its pharmacological potential [ 20 ]. However, elucidation of the functional role and cellular mechanisms by which compounds from Dracaena trifasciata may modulate the metabolic reprogramming in RA remains untapped in the literature.…”

Section: Introductionmentioning

confidence: 87%

Docking and Molecular Dynamics Study to Identify Novel Phytobiologics from Dracaena trifasciata against Metabolic Reprogramming in Rheumatoid Arthritis

Ahmed

John

Paracha

et al. 2022

Life

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Enhancement of glycolysis and glutaminolysis are the two most common modalities associated with metabolic reprogramming in rheumatoid arthritis (RA). This enhancement is concomitant to the upregulation of hexokinase 2 (HK2) and glutaminase 1 (GLS1). Hence, the current study was undertaken to identify potential phytobiological inhibitors against HK2 and GLS1, from Dracaena (Sansevieria) trifasciata, an indigenous ethnomedicinal plant found in Pakistan, using computational analysis. Phytobiologics from Dracaena trifasciata were assessed for their ability to co-inhibit HK2 and GLS1 via molecular docking and molecular dynamics simulations. The results underscored seven phytobiologics with promising binding affinities for both HK2 and GLS1. Molecular dynamics simulations further elucidated that all seven identified phytobiologics inhibited HK2 by forming stable complexes but only five amongst the seven had the potential to form stable complexes with GLS1 in real time, thereby implying the potential of co-inhibition for these five compounds. Compound 28MS exhibited an equally strong binding profile for both HK2 (−8.19 kcal/mol) and GLS1 (−8.99 kcal/mol). Furthermore, it exhibited a similar trend in stability during simulation for both targets. Our results serve as a primer for a more lucid understanding towards co-inhibition of HK2 and GLS1 using multiple computational approaches. The identified phytobiologics should undergo in-vitro and in-vivo validation to corroborate their therapeutic potential in RA.

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“…Bu sebeple yapılan çalışmalar yoğunlukla kanser tedavisinde kullanılabilecek nitelikte olan ilaç etken maddeleri üzerinde yoğunlaşmıştır. 4,5 Tıbbi bitkiler; tedavi amaçlı kullanımı ön planda olan ilaç formülasyonlarına etken madde sağlamak için sınırsız imkanlar sunan, önemli ana kaynaklar olarak kabul edilmektedir ve klinik modern ilaç etken maddelerin yaklaşık %50'si bitkisel kökenlidir. 4 Özellikle son yıllarda kanser hastalıklarında tedavi amaçlı kullanılan bitkiler, sahip oldukları sekonder metabolitler sebebiyle oldukça detaylı bir şekilde çalışılmaya başlanmıştır.…”

Section: 2unclassified

Alchemilla mollis (Buser) Rothm bitkisi köklerinden elde edilen ekstrelerin antioksidan ve sitotoksik etkileri

İlgün

Karatoprak

2021

Mersin Üniversitesi Sağlık Bilimleri Dergisi

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Amaç: Alchemilla L. cinsine ait türlerin genellikle toprak üstü kısımlarının halk arasında diüretik, astrenjan, antispazmodik, yara iyileştirici, aşırı menstürasyonda kür olarak ve konvulsif hastalıkların tedavisinde kullanılmakta olduğu bilinmektedir. Sahip oldukları zengin polifenol içeriği sebebiyle de antioksidan, antimikrobiyal ve antienflamatuvar etkiler gösterdiği belirlenmiştir. Bu çalışmada ise cinse ait türlerden A. mollis bitkisinin köklerinden hazırlanan ekstrelerin antioksidan ve sitotoksik etkilerinin belirlenmesi için çeşitli aktivite testleri yapılarak, etkinliğinin tespit edilmesi amaçlanmıştır. Yöntem: Bu çalışmada A. mollis türünün köklerinden hazırlanan metanol ekstresi ve bu ekstrenin partisyonu ile elde edilen farklı polariteye sahip alt ekstrelerin toplam fenol ve flavonoit miktar tayini ve antioksidan kapasitelerini belirlemek için DPPH ve ABTS radikalini süpürücü etkileri tespit edilmiştir. Ayrıca ekstrelerin L929 ve MDA-MB-231 hücre hatlarında sitotoksik etkileri değerlendirilmiştir. Bulgular: Yüksek fenolik içeriğine bağlı olarak, bitkinin kök ekstrelerinde yüksek antioksidan kapasite tespit edilmiştir. Bitkinin köklerinden elde edilen ekstrelerin L929 sağlıklı fare fibroblast hücrelerinde ve MDA-MB-231 meme kanseri hücre hattında elde edilen sonuçlara göre; ekstreler belli bir konsantrasyon aralığında sağlıklı hücrelere toksik etki göstermezken meme kanseri hücreleri üzerinde belirli dozlarda toksik etkili bulunmuştur. Sonuç: Sonuç olarak A. mollis bitkisi zengin fenolik bileşik içeriği ile kanserli hücrelerin poliferasyonunu inhibe ederek kanser tedavisinde umut verici terapötik ajanların keşfedilmesinde önemli bir kaynak olabileceği tarafımızca yapılan çalışmalarla tespit edilmiştir

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In vitro cytotoxic activity of certain succulent plants against human colon, breast and liver cancer cell lines

Cited by 8 publications

References 29 publications

Sansevieria: An evaluation of cytotoxic activity in reference to metabolomic and molecular docking studies

Sansevieria: An evaluation of cytotoxic activity in reference to metabolomic and molecular docking studies

Docking and Molecular Dynamics Study to Identify Novel Phytobiologics from Dracaena trifasciata against Metabolic Reprogramming in Rheumatoid Arthritis

Alchemilla mollis (Buser) Rothm bitkisi köklerinden elde edilen ekstrelerin antioksidan ve sitotoksik etkileri

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