In vitro culture of fennel tissues (Foeniculum vulgare Miller) from cell suspension to mature plant

Hunault, G.

doi:10.1016/0304-4238(84)90083-9

Cited by 29 publications

(9 citation statements)

References 21 publications

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“…In vitro-derived roots are often delicate and easily damaged during transplantation and often show limited physiological functioning when in contact with the soil (Gangopadhyay et al, 2002). An additional complication to the tissue culture of herbaceous plants of the Umbellifereae is their poor establishment in soil after in vitro culture due to their susceptibility to fungal rot or wilt diseases as evidenced in Ammi visgana (Ekiert, 2000); Cuminum cyminum (Tawfik and Noga, 2001); T. garganica; (Makunga et al, 2003) and their sensitivity to water saturation (Hunault, 1984). In the field cultivation of carrots, fungal disease management involves thorough foliar spray coverage with fungicides (Douglas and Cowley, 2003) as soon as symptoms are visible.…”

Section: Rooting and Acclimatizationmentioning

confidence: 99%

An improved system for the in vitro regeneration of Thapsia garganica via direct organogenesis – influence of auxins and cytokinins

Makunga

Jäger

Staden

2005

Plant Cell Tiss Organ Cult

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An improved protocol for mass multiplication directly from leaf material of Thapsia garganica was developed. Using factorial experimentation, auxins (NAA, IAA, 2,4-D D ) and cytokinin (BA, kinetin) combinations at 0-2 mg l )1 added to Murashige and Skoog (MS) medium with 30 g l )1 sucrose and 8 g l )1 agar (pH 5.8) were tested for their effect on direct regeneration on leaflet and petiole explants. Of the media tested, the 0.5:1.5 NAA:BA medium was comparable for direct shoot organogenesis to the 2 mg l )1 kinetin supplemented medium. However, when shoots were multiplied on these media, the 2 mg l )1 kinetin without auxins was most effective as it kept the percentage of callus-derived plantlets to 3% and the number of hyperhydric shoots were minimal at a frequency of 2% compared to 25% on the 0.5:1.5 NAA:BA medium. The 2 mg l )1 kinetin medium induced adventitious bud formation in 36% of the explants after 30 days. When the cultures were transferred to the same medium for multiplication, an average of six shoots (4.3 cm) were derived from each shoot base. Other combinations resulted in callus formation that either preceded shoot production or occurred together with adventitious shoot induction; whereas the 2 mg l )1 medium resulted solely in adventitious buds that readily converted and elongated to shoots. On the 0.5:1.5 NAA:BA medium which tended to induce hyperhydric shoots in culture, agar (0.8%, [w/v]) (15% hyperhydric plantlets) was more useful in maintaining a high health status in regenerating plantlets than gellan gum (Gelrite Ò ; 0.25%, [w/v]) (60% hyperhydric plantlets). Although rooting in vitro was difficult, 58% of the propagules were successfully acclimatized when plants were exposed to fungicidal solutions as pre-and post-acclimatization treatments. A comprehensive protocol that allows for a reduction in mortality due to damping-off diseases during ex vitro transplantation of the in vitro-derived T. garganica plantlets is reported. The acclimatization procedure presented here is potentially suited to other umbelliferous species where fungal rots hamper ex vitro establishment.

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Section: Rooting and Acclimatizationmentioning

confidence: 99%

An improved system for the in vitro regeneration of Thapsia garganica via direct organogenesis – influence of auxins and cytokinins

Makunga

Jäger

Staden

2005

Plant Cell Tiss Organ Cult

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“…al., 1985), establishment of callus (Paupardin et al, 1980;Hunault, 1981), suspension cultures (Hunault, 1984) and the capacity to produce plantlets via somatic embryogenesis in vitro (Maheshwari and Gupta, 1965;Hunault, 1984;Theiler-Hedtrich and K~igi, 1991;Hunault and Maatar, 1995).…”

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confidence: 99%

In vitro culture of Foeniculum vulgare: callus characteristics in relation to morphogenesis

Anzidei

Vivona

Schiff

et al. 1996

Plant Cell Tiss Organ Cult

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“…Their frequently rapid proliferation leads to the appearance of friable colonies (secondary calli). When transferred to a medium devoid of auxin (development medium), these tissues give embryos that evolve directly into plantlets (Hunault 1981(Hunault , 1984 (Fig. 2).…”

Section: Development Of Embryogenic Tissuesmentioning

confidence: 99%

“…Nevertheless, a large number of these plantlets display more or less extensive malformations and cannot be further utilized (Hunault 1981(Hunault , 1984. The plantlets altered through hyperhydric transformation seem to be more numerous on MS medium than on Shah and Dalal (Du Manoir, unpubl.).…”

Section: Development Of Embryo-originated Plantletsmentioning

confidence: 99%

“…c) To obtain embryos and plantlets, transfer embryogenic tissues to a medium lacking growth regulator and containing Shah and Dalal (1980) macroelements (or half-strength MS macroelements), 0.4 mg/1 thiamine HCI, and 2% glucose. Better plantlet development can be obtained by first growing the embryogenic tissues in a liquid medium and then laying them out onto a solidified development medium (Hunault 1984).…”

Section: Somatic Embryogenesismentioning

confidence: 99%

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Micropropagation of Fennel (Foeniculum vulgare Miller)

Hunault¹,

Manoir

1992

High-Tech and Micropropagation III

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In vitro culture of fennel tissues (Foeniculum vulgare Miller) from cell suspension to mature plant

Cited by 29 publications

References 21 publications

An improved system for the in vitro regeneration of Thapsia garganica via direct organogenesis – influence of auxins and cytokinins

An improved system for the in vitro regeneration of Thapsia garganica via direct organogenesis – influence of auxins and cytokinins

In vitro culture of Foeniculum vulgare: callus characteristics in relation to morphogenesis

Micropropagation of Fennel (Foeniculum vulgare Miller)

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